Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and gpt are separated by 91 nucleotides gpt and pepD are separated by 260 nucleotides pepD and prfH are separated by 56 nucleotides prfH and rtcB overlap by 4 nucleotides
HEPCGN_07795: frsA - esterase FrsA, at 301,354 to 302,598
frsA
HEPCGN_07800: gpt - xanthine phosphoribosyltransferase, at 302,690 to 303,148
gpt
HEPCGN_07805: pepD - cytosol nonspecific dipeptidase, at 303,409 to 304,866
pepD
HEPCGN_07810: prfH - peptide chain release factor H, at 304,923 to 305,537
prfH
HEPCGN_07815: rtcB - RNA ligase RtcB family protein, at 305,534 to 306,673
rtcB
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1
2 at 302.476 kb on - strand at 302.517 kb on + strand at 302.530 kb on + strand at 302.553 kb on + strand at 302.553 kb on + strand at 302.620 kb on + strand at 302.631 kb on - strand at 302.962 kb on - strand, within gpt at 302.962 kb on - strand, within gpt at 303.052 kb on + strand, within gpt at 303.098 kb on - strand, within gpt at 303.136 kb on - strand at 303.174 kb on - strand at 303.181 kb on - strand at 303.202 kb on + strand at 303.217 kb on + strand at 303.219 kb on + strand at 303.220 kb on - strand at 303.246 kb on - strand at 303.246 kb on - strand at 303.317 kb on + strand at 303.318 kb on - strand at 303.318 kb on - strand at 303.374 kb on + strand at 303.405 kb on + strand at 303.405 kb on + strand at 303.405 kb on + strand at 303.406 kb on - strand at 303.428 kb on + strand at 303.428 kb on + strand at 303.471 kb on + strand at 303.471 kb on + strand at 303.472 kb on - strand at 303.472 kb on - strand at 303.472 kb on - strand at 303.564 kb on - strand, within pepD at 303.564 kb on - strand, within pepD at 303.567 kb on + strand, within pepD at 303.568 kb on - strand, within pepD at 303.600 kb on + strand, within pepD at 303.600 kb on + strand, within pepD at 303.661 kb on - strand, within pepD at 303.684 kb on + strand, within pepD at 303.704 kb on - strand, within pepD at 303.706 kb on - strand, within pepD at 303.766 kb on - strand, within pepD at 303.937 kb on + strand, within pepD at 303.937 kb on + strand, within pepD at 303.938 kb on - strand, within pepD at 303.938 kb on - strand, within pepD at 303.983 kb on + strand, within pepD at 304.031 kb on + strand, within pepD at 304.032 kb on - strand, within pepD at 304.032 kb on - strand, within pepD at 304.251 kb on + strand, within pepD at 304.251 kb on + strand, within pepD at 304.252 kb on - strand, within pepD at 304.255 kb on + strand, within pepD at 304.476 kb on + strand, within pepD at 304.477 kb on - strand, within pepD at 304.481 kb on + strand, within pepD at 304.482 kb on - strand, within pepD at 304.482 kb on - strand, within pepD at 304.617 kb on + strand, within pepD at 304.651 kb on + strand, within pepD at 304.652 kb on - strand, within pepD at 304.950 kb on - strand at 305.160 kb on - strand, within prfH at 305.162 kb on + strand, within prfH at 305.319 kb on - strand, within prfH at 305.526 kb on + strand at 305.646 kb on + strand at 305.646 kb on + strand at 305.736 kb on + strand, within rtcB at 305.758 kb on + strand, within rtcB at 305.833 kb on + strand, within rtcB at 305.848 kb on - strand, within rtcB at 305.848 kb on - strand, within rtcB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 302,476 - +0.3 302,517 + -0.6 302,530 + -0.3 302,553 + +0.1 302,553 + -0.4 302,620 + +0.4 302,631 - -0.5 302,962 - gpt HEPCGN_07800 0.59 +0.1 302,962 - gpt HEPCGN_07800 0.59 -0.3 303,052 + gpt HEPCGN_07800 0.79 -0.6 303,098 - gpt HEPCGN_07800 0.89 -1.4 303,136 - -0.0 303,174 - +0.0 303,181 - +0.5 303,202 + +0.1 303,217 + -0.1 303,219 + -0.2 303,220 - -0.3 303,246 - -1.2 303,246 - +0.9 303,317 + +0.2 303,318 - -0.5 303,318 - +0.3 303,374 + +0.4 303,405 + +0.0 303,405 + +0.2 303,405 + -0.1 303,406 - +0.1 303,428 + -1.4 303,428 + -0.2 303,471 + +0.0 303,471 + -0.1 303,472 - +0.4 303,472 - -1.1 303,472 - +0.0 303,564 - pepD HEPCGN_07805 0.11 +0.3 303,564 - pepD HEPCGN_07805 0.11 +0.2 303,567 + pepD HEPCGN_07805 0.11 -2.0 303,568 - pepD HEPCGN_07805 0.11 +0.4 303,600 + pepD HEPCGN_07805 0.13 +0.4 303,600 + pepD HEPCGN_07805 0.13 +0.1 303,661 - pepD HEPCGN_07805 0.17 +0.1 303,684 + pepD HEPCGN_07805 0.19 +1.8 303,704 - pepD HEPCGN_07805 0.20 +0.0 303,706 - pepD HEPCGN_07805 0.20 -0.6 303,766 - pepD HEPCGN_07805 0.24 -0.2 303,937 + pepD HEPCGN_07805 0.36 +0.8 303,937 + pepD HEPCGN_07805 0.36 -0.4 303,938 - pepD HEPCGN_07805 0.36 +0.5 303,938 - pepD HEPCGN_07805 0.36 +1.7 303,983 + pepD HEPCGN_07805 0.39 +0.3 304,031 + pepD HEPCGN_07805 0.43 -0.4 304,032 - pepD HEPCGN_07805 0.43 +0.1 304,032 - pepD HEPCGN_07805 0.43 +0.7 304,251 + pepD HEPCGN_07805 0.58 +0.2 304,251 + pepD HEPCGN_07805 0.58 -1.2 304,252 - pepD HEPCGN_07805 0.58 -0.8 304,255 + pepD HEPCGN_07805 0.58 +0.4 304,476 + pepD HEPCGN_07805 0.73 -0.2 304,477 - pepD HEPCGN_07805 0.73 +0.4 304,481 + pepD HEPCGN_07805 0.74 -0.8 304,482 - pepD HEPCGN_07805 0.74 -0.3 304,482 - pepD HEPCGN_07805 0.74 -0.3 304,617 + pepD HEPCGN_07805 0.83 +0.3 304,651 + pepD HEPCGN_07805 0.85 -0.3 304,652 - pepD HEPCGN_07805 0.85 +2.0 304,950 - +0.2 305,160 - prfH HEPCGN_07810 0.39 -0.5 305,162 + prfH HEPCGN_07810 0.39 +0.7 305,319 - prfH HEPCGN_07810 0.64 +0.6 305,526 + +0.3 305,646 + +0.0 305,646 + -0.5 305,736 + rtcB HEPCGN_07815 0.18 -0.4 305,758 + rtcB HEPCGN_07815 0.20 +0.8 305,833 + rtcB HEPCGN_07815 0.26 +0.1 305,848 - rtcB HEPCGN_07815 0.28 -0.2 305,848 - rtcB HEPCGN_07815 0.28 -0.3
Or see this region's nucleotide sequence