Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and HEPCGN_05455 are separated by 4 nucleotides HEPCGN_05455 and btuC are separated by 100 nucleotides btuC and btuE are separated by 62 nucleotides btuE and btuD overlap by 1 nucleotides
HEPCGN_05450: pheT - phenylalanine--tRNA ligase subunit beta, at 963,230 to 965,617
pheT
HEPCGN_05455: HEPCGN_05455 - Integration host factor subunit alpha, at 965,622 to 965,921
_05455
HEPCGN_05465: btuC - vitamin B12 ABC transporter permease BtuC, at 966,022 to 967,002
btuC
HEPCGN_05470: btuE - bifunctional thioredoxin/glutathione peroxidase, at 967,065 to 967,616
btuE
HEPCGN_05475: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 967,616 to 968,365
btuD
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1 at 966.210 kb on - strand, within btuC at 966.291 kb on - strand, within btuC at 966.411 kb on - strand, within btuC at 966.420 kb on - strand, within btuC at 966.490 kb on - strand, within btuC at 966.498 kb on - strand, within btuC at 966.498 kb on - strand, within btuC at 966.498 kb on - strand, within btuC at 966.536 kb on + strand, within btuC at 966.657 kb on + strand, within btuC at 966.657 kb on + strand, within btuC at 966.658 kb on - strand, within btuC at 966.845 kb on + strand, within btuC at 966.845 kb on + strand, within btuC at 966.846 kb on - strand, within btuC at 966.846 kb on - strand, within btuC at 966.914 kb on + strand at 966.987 kb on - strand at 967.020 kb on - strand at 967.020 kb on - strand at 967.131 kb on + strand, within btuE at 967.192 kb on - strand, within btuE at 967.302 kb on - strand, within btuE at 967.309 kb on - strand, within btuE at 967.480 kb on - strand, within btuE at 967.480 kb on - strand, within btuE at 967.614 kb on + strand at 967.621 kb on + strand at 967.741 kb on - strand, within btuD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 966,210 - btuC HEPCGN_05465 0.19 -0.3 966,291 - btuC HEPCGN_05465 0.27 -0.1 966,411 - btuC HEPCGN_05465 0.40 -0.2 966,420 - btuC HEPCGN_05465 0.41 -0.3 966,490 - btuC HEPCGN_05465 0.48 +0.0 966,498 - btuC HEPCGN_05465 0.49 -0.1 966,498 - btuC HEPCGN_05465 0.49 -0.5 966,498 - btuC HEPCGN_05465 0.49 +0.3 966,536 + btuC HEPCGN_05465 0.52 -0.7 966,657 + btuC HEPCGN_05465 0.65 -1.9 966,657 + btuC HEPCGN_05465 0.65 -0.3 966,658 - btuC HEPCGN_05465 0.65 -0.4 966,845 + btuC HEPCGN_05465 0.84 -0.6 966,845 + btuC HEPCGN_05465 0.84 -0.2 966,846 - btuC HEPCGN_05465 0.84 -0.4 966,846 - btuC HEPCGN_05465 0.84 +0.5 966,914 + +0.4 966,987 - -1.3 967,020 - +1.0 967,020 - +0.0 967,131 + btuE HEPCGN_05470 0.12 -2.1 967,192 - btuE HEPCGN_05470 0.23 -0.1 967,302 - btuE HEPCGN_05470 0.43 -0.4 967,309 - btuE HEPCGN_05470 0.44 +0.7 967,480 - btuE HEPCGN_05470 0.75 +0.1 967,480 - btuE HEPCGN_05470 0.75 +0.3 967,614 + -0.1 967,621 + +0.2 967,741 - btuD HEPCGN_05475 0.17 -0.8
Or see this region's nucleotide sequence