Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03220

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisC and hisD overlap by 4 nucleotideshisD and hisG are separated by 5 nucleotides HEPCGN_03215: hisC - histidinol-phosphate transaminase, at 554,614 to 555,684 hisC HEPCGN_03220: hisD - Histidinol dehydrogenase, at 555,681 to 556,985 hisD HEPCGN_03225: hisG - ATP phosphoribosyltransferase, at 556,991 to 557,890 hisG Position (kb) 555 556 557Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 554.722 kb on + strand, within hisCat 554.727 kb on + strand, within hisCat 554.789 kb on + strand, within hisCat 554.790 kb on - strand, within hisCat 554.853 kb on - strand, within hisCat 555.125 kb on + strand, within hisCat 555.126 kb on - strand, within hisCat 555.233 kb on + strand, within hisCat 555.233 kb on + strand, within hisCat 555.348 kb on - strand, within hisCat 555.424 kb on - strand, within hisCat 555.467 kb on - strand, within hisCat 555.476 kb on + strand, within hisCat 555.486 kb on - strand, within hisCat 555.544 kb on - strand, within hisCat 555.669 kb on + strandat 555.670 kb on - strandat 555.704 kb on + strandat 555.793 kb on - strandat 555.865 kb on + strand, within hisDat 555.871 kb on + strand, within hisDat 556.292 kb on + strand, within hisDat 556.323 kb on + strand, within hisDat 556.598 kb on - strand, within hisDat 556.598 kb on - strand, within hisDat 556.598 kb on - strand, within hisDat 556.615 kb on - strand, within hisDat 556.641 kb on + strand, within hisDat 556.659 kb on + strand, within hisDat 556.659 kb on + strand, within hisDat 556.704 kb on - strand, within hisDat 556.734 kb on + strand, within hisDat 556.735 kb on - strand, within hisDat 556.825 kb on + strand, within hisDat 556.955 kb on + strandat 556.955 kb on + strandat 556.956 kb on - strandat 556.976 kb on + strandat 557.102 kb on + strand, within hisGat 557.134 kb on + strand, within hisGat 557.208 kb on + strand, within hisGat 557.329 kb on + strand, within hisGat 557.329 kb on + strand, within hisGat 557.330 kb on - strand, within hisGat 557.353 kb on + strand, within hisGat 557.442 kb on - strand, within hisGat 557.499 kb on + strand, within hisGat 557.512 kb on + strand, within hisGat 557.800 kb on + strand, within hisGat 557.865 kb on - strandat 557.896 kb on + strandat 557.897 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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554,722 + hisC HEPCGN_03215 0.10 -3.5
554,727 + hisC HEPCGN_03215 0.11 -2.6
554,789 + hisC HEPCGN_03215 0.16 -7.5
554,790 - hisC HEPCGN_03215 0.16 -4.2
554,853 - hisC HEPCGN_03215 0.22 -4.6
555,125 + hisC HEPCGN_03215 0.48 -4.8
555,126 - hisC HEPCGN_03215 0.48 -3.5
555,233 + hisC HEPCGN_03215 0.58 -3.0
555,233 + hisC HEPCGN_03215 0.58 -4.6
555,348 - hisC HEPCGN_03215 0.69 -3.1
555,424 - hisC HEPCGN_03215 0.76 -4.8
555,467 - hisC HEPCGN_03215 0.80 -4.2
555,476 + hisC HEPCGN_03215 0.80 -4.7
555,486 - hisC HEPCGN_03215 0.81 -2.9
555,544 - hisC HEPCGN_03215 0.87 -3.6
555,669 + -2.5
555,670 - -5.3
555,704 + -0.8
555,793 - -4.5
555,865 + hisD HEPCGN_03220 0.14 -4.0
555,871 + hisD HEPCGN_03220 0.15 -2.6
556,292 + hisD HEPCGN_03220 0.47 -4.5
556,323 + hisD HEPCGN_03220 0.49 -3.9
556,598 - hisD HEPCGN_03220 0.70 -4.0
556,598 - hisD HEPCGN_03220 0.70 -2.8
556,598 - hisD HEPCGN_03220 0.70 -3.8
556,615 - hisD HEPCGN_03220 0.72 -3.5
556,641 + hisD HEPCGN_03220 0.74 -6.0
556,659 + hisD HEPCGN_03220 0.75 -0.1
556,659 + hisD HEPCGN_03220 0.75 -2.8
556,704 - hisD HEPCGN_03220 0.78 -3.8
556,734 + hisD HEPCGN_03220 0.81 -2.7
556,735 - hisD HEPCGN_03220 0.81 -4.8
556,825 + hisD HEPCGN_03220 0.88 -3.3
556,955 + -2.4
556,955 + -4.4
556,956 - -0.5
556,976 + -0.1
557,102 + hisG HEPCGN_03225 0.12 -3.0
557,134 + hisG HEPCGN_03225 0.16 -3.6
557,208 + hisG HEPCGN_03225 0.24 -0.8
557,329 + hisG HEPCGN_03225 0.38 -5.1
557,329 + hisG HEPCGN_03225 0.38 -2.4
557,330 - hisG HEPCGN_03225 0.38 -4.3
557,353 + hisG HEPCGN_03225 0.40 -3.9
557,442 - hisG HEPCGN_03225 0.50 -4.6
557,499 + hisG HEPCGN_03225 0.56 -4.1
557,512 + hisG HEPCGN_03225 0.58 -2.2
557,800 + hisG HEPCGN_03225 0.90 -4.0
557,865 - -5.4
557,896 + -4.4
557,897 - -2.1

Or see this region's nucleotide sequence