Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hutI and hutG overlap by 1 nucleotides hutG and HEPCGN_01360 are separated by 50 nucleotides
HEPCGN_01350: hutI - imidazolonepropionase, at 198,399 to 199,610
hutI
HEPCGN_01355: hutG - formimidoylglutamase, at 199,610 to 200,560
hutG
HEPCGN_01360: HEPCGN_01360 - histidine ammonia-lyase, at 200,611 to 202,281
_01360
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 198.611 kb on + strand, within hutI at 198.625 kb on - strand, within hutI at 198.709 kb on + strand, within hutI at 198.710 kb on - strand, within hutI at 198.713 kb on + strand, within hutI at 198.713 kb on + strand, within hutI at 198.713 kb on + strand, within hutI at 198.714 kb on - strand, within hutI at 198.714 kb on - strand, within hutI at 198.804 kb on - strand, within hutI at 198.888 kb on - strand, within hutI at 198.895 kb on + strand, within hutI at 198.895 kb on + strand, within hutI at 198.895 kb on + strand, within hutI at 198.896 kb on - strand, within hutI at 198.963 kb on + strand, within hutI at 198.964 kb on - strand, within hutI at 198.997 kb on + strand, within hutI at 198.998 kb on - strand, within hutI at 199.066 kb on + strand, within hutI at 199.095 kb on + strand, within hutI at 199.102 kb on + strand, within hutI at 199.103 kb on - strand, within hutI at 199.108 kb on + strand, within hutI at 199.194 kb on + strand, within hutI at 199.267 kb on - strand, within hutI at 199.392 kb on - strand, within hutI at 199.400 kb on + strand, within hutI at 199.448 kb on + strand, within hutI at 199.530 kb on - strand at 199.763 kb on - strand, within hutG at 199.920 kb on + strand, within hutG at 199.921 kb on - strand, within hutG at 199.924 kb on + strand, within hutG at 199.924 kb on + strand, within hutG at 199.947 kb on + strand, within hutG at 200.002 kb on + strand, within hutG at 200.003 kb on - strand, within hutG at 200.003 kb on - strand, within hutG at 200.191 kb on - strand, within hutG at 200.338 kb on - strand, within hutG at 200.485 kb on + strand at 200.741 kb on + strand at 200.846 kb on + strand, within HEPCGN_01360 at 200.868 kb on + strand, within HEPCGN_01360 at 201.017 kb on + strand, within HEPCGN_01360 at 201.027 kb on + strand, within HEPCGN_01360 at 201.028 kb on - strand, within HEPCGN_01360 at 201.030 kb on + strand, within HEPCGN_01360 at 201.031 kb on - strand, within HEPCGN_01360 at 201.062 kb on - strand, within HEPCGN_01360 at 201.062 kb on - strand, within HEPCGN_01360 at 201.076 kb on + strand, within HEPCGN_01360 at 201.087 kb on + strand, within HEPCGN_01360 at 201.087 kb on + strand, within HEPCGN_01360 at 201.088 kb on - strand, within HEPCGN_01360 at 201.103 kb on - strand, within HEPCGN_01360 at 201.201 kb on + strand, within HEPCGN_01360 at 201.250 kb on - strand, within HEPCGN_01360 at 201.276 kb on + strand, within HEPCGN_01360 at 201.277 kb on - strand, within HEPCGN_01360 at 201.316 kb on + strand, within HEPCGN_01360 at 201.488 kb on + strand, within HEPCGN_01360 at 201.500 kb on - strand, within HEPCGN_01360 at 201.510 kb on + strand, within HEPCGN_01360 at 201.510 kb on + strand, within HEPCGN_01360
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 198,611 + hutI HEPCGN_01350 0.17 +0.7 198,625 - hutI HEPCGN_01350 0.19 -0.0 198,709 + hutI HEPCGN_01350 0.26 +0.1 198,710 - hutI HEPCGN_01350 0.26 +0.1 198,713 + hutI HEPCGN_01350 0.26 +0.4 198,713 + hutI HEPCGN_01350 0.26 +0.0 198,713 + hutI HEPCGN_01350 0.26 -0.3 198,714 - hutI HEPCGN_01350 0.26 -0.1 198,714 - hutI HEPCGN_01350 0.26 +0.5 198,804 - hutI HEPCGN_01350 0.33 +0.4 198,888 - hutI HEPCGN_01350 0.40 -0.4 198,895 + hutI HEPCGN_01350 0.41 +0.1 198,895 + hutI HEPCGN_01350 0.41 -0.1 198,895 + hutI HEPCGN_01350 0.41 +0.0 198,896 - hutI HEPCGN_01350 0.41 +0.3 198,963 + hutI HEPCGN_01350 0.47 +0.4 198,964 - hutI HEPCGN_01350 0.47 +0.5 198,997 + hutI HEPCGN_01350 0.49 +0.3 198,998 - hutI HEPCGN_01350 0.49 -0.1 199,066 + hutI HEPCGN_01350 0.55 +0.8 199,095 + hutI HEPCGN_01350 0.57 +0.2 199,102 + hutI HEPCGN_01350 0.58 +0.4 199,103 - hutI HEPCGN_01350 0.58 -1.0 199,108 + hutI HEPCGN_01350 0.58 -0.0 199,194 + hutI HEPCGN_01350 0.66 -0.0 199,267 - hutI HEPCGN_01350 0.72 +0.4 199,392 - hutI HEPCGN_01350 0.82 -0.2 199,400 + hutI HEPCGN_01350 0.83 +0.0 199,448 + hutI HEPCGN_01350 0.87 +1.1 199,530 - -0.1 199,763 - hutG HEPCGN_01355 0.16 -0.3 199,920 + hutG HEPCGN_01355 0.33 -0.6 199,921 - hutG HEPCGN_01355 0.33 -3.1 199,924 + hutG HEPCGN_01355 0.33 -1.8 199,924 + hutG HEPCGN_01355 0.33 -1.1 199,947 + hutG HEPCGN_01355 0.35 +0.4 200,002 + hutG HEPCGN_01355 0.41 -0.1 200,003 - hutG HEPCGN_01355 0.41 -0.1 200,003 - hutG HEPCGN_01355 0.41 +0.1 200,191 - hutG HEPCGN_01355 0.61 -0.5 200,338 - hutG HEPCGN_01355 0.77 +2.4 200,485 + -0.3 200,741 + +0.8 200,846 + HEPCGN_01360 0.14 +0.4 200,868 + HEPCGN_01360 0.15 +1.2 201,017 + HEPCGN_01360 0.24 +0.9 201,027 + HEPCGN_01360 0.25 +0.8 201,028 - HEPCGN_01360 0.25 +0.4 201,030 + HEPCGN_01360 0.25 +0.5 201,031 - HEPCGN_01360 0.25 +1.6 201,062 - HEPCGN_01360 0.27 +0.5 201,062 - HEPCGN_01360 0.27 +0.3 201,076 + HEPCGN_01360 0.28 +0.5 201,087 + HEPCGN_01360 0.28 -0.3 201,087 + HEPCGN_01360 0.28 -0.4 201,088 - HEPCGN_01360 0.29 +1.4 201,103 - HEPCGN_01360 0.29 +0.5 201,201 + HEPCGN_01360 0.35 +0.2 201,250 - HEPCGN_01360 0.38 -0.1 201,276 + HEPCGN_01360 0.40 +0.0 201,277 - HEPCGN_01360 0.40 +0.5 201,316 + HEPCGN_01360 0.42 +0.2 201,488 + HEPCGN_01360 0.52 +0.6 201,500 - HEPCGN_01360 0.53 -0.5 201,510 + HEPCGN_01360 0.54 +0.4 201,510 + HEPCGN_01360 0.54 +0.2
Or see this region's nucleotide sequence