Strain Fitness in Escherichia coli ECOR27 around NOLOHH_24225

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttraL and traA are separated by 14 nucleotidestraA and traY are separated by 42 nucleotidestraY and traJ are separated by 135 nucleotidestraJ and NOLOHH_24235 are separated by 190 nucleotides NOLOHH_24210: traL - type IV conjugative transfer system protein TraL, at 528 to 839 traL NOLOHH_24215: traA - type IV conjugative transfer system pilin TraA, at 854 to 1,219 traA NOLOHH_24220: traY - conjugal transfer relaxosome protein TraY, at 1,262 to 1,477 traY NOLOHH_24225: traJ - conjugal transfer transcriptional regulator TraJ, at 1,613 to 2,260 traJ NOLOHH_24235: NOLOHH_24235 - conjugal transfer protein TraM, at 2,451 to 2,834 _24235 Position (kb) 1 2 3Strain fitness (log2 ratio) -3 -2 -1 0 1at 0.887 kb on - strandat 0.887 kb on - strandat 0.887 kb on - strandat 0.891 kb on - strand, within traAat 0.942 kb on + strand, within traAat 0.943 kb on - strand, within traAat 1.206 kb on - strandat 1.220 kb on - strandat 1.998 kb on - strand, within traJat 2.260 kb on + strandat 2.260 kb on + strandat 2.261 kb on - strandat 2.261 kb on - strandat 2.261 kb on - strandat 2.261 kb on - strandat 2.369 kb on + strandat 2.371 kb on + strandat 2.511 kb on - strand, within NOLOHH_24235at 2.564 kb on + strand, within NOLOHH_24235

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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887 - +1.5
887 - +0.7
887 - -2.3
891 - traA NOLOHH_24215 0.10 -0.8
942 + traA NOLOHH_24215 0.24 -1.2
943 - traA NOLOHH_24215 0.24 +0.4
1,206 - -0.2
1,220 - -1.0
1,998 - traJ NOLOHH_24225 0.59 -3.6
2,260 + -0.6
2,260 + +1.5
2,261 - +0.2
2,261 - +1.3
2,261 - +1.0
2,261 - +0.8
2,369 + -0.4
2,371 + -0.4
2,511 - NOLOHH_24235 0.16 -0.9
2,564 + NOLOHH_24235 0.29 -0.3

Or see this region's nucleotide sequence