Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt traL and traA are separated by 14 nucleotides traA and traY are separated by 42 nucleotides traY and traJ are separated by 135 nucleotides traJ and NOLOHH_24235 are separated by 190 nucleotides
NOLOHH_24210: traL - type IV conjugative transfer system protein TraL, at 528 to 839
traL
NOLOHH_24215: traA - type IV conjugative transfer system pilin TraA, at 854 to 1,219
traA
NOLOHH_24220: traY - conjugal transfer relaxosome protein TraY, at 1,262 to 1,477
traY
NOLOHH_24225: traJ - conjugal transfer transcriptional regulator TraJ, at 1,613 to 2,260
traJ
NOLOHH_24235: NOLOHH_24235 - conjugal transfer protein TraM, at 2,451 to 2,834
_24235
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 0.887 kb on - strand at 0.887 kb on - strand at 0.887 kb on - strand at 0.891 kb on - strand, within traA at 0.942 kb on + strand, within traA at 0.943 kb on - strand, within traA at 1.206 kb on - strand at 1.220 kb on - strand at 1.998 kb on - strand, within traJ at 2.260 kb on + strand at 2.260 kb on + strand at 2.261 kb on - strand at 2.261 kb on - strand at 2.261 kb on - strand at 2.261 kb on - strand at 2.369 kb on + strand at 2.371 kb on + strand at 2.511 kb on - strand, within NOLOHH_24235 at 2.564 kb on + strand, within NOLOHH_24235
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 887 - +1.5 887 - +0.7 887 - -2.3 891 - traA NOLOHH_24215 0.10 -0.8 942 + traA NOLOHH_24215 0.24 -1.2 943 - traA NOLOHH_24215 0.24 +0.4 1,206 - -0.2 1,220 - -1.0 1,998 - traJ NOLOHH_24225 0.59 -3.6 2,260 + -0.6 2,260 + +1.5 2,261 - +0.2 2,261 - +1.3 2,261 - +1.0 2,261 - +0.8 2,369 + -0.4 2,371 + -0.4 2,511 - NOLOHH_24235 0.16 -0.9 2,564 + NOLOHH_24235 0.29 -0.3
Or see this region's nucleotide sequence