Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmC and ybjO are separated by 40 nucleotides ybjO and potI are separated by 59 nucleotides potI and potH overlap by 4 nucleotides
NOLOHH_22305: rlmC - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 4,403,419 to 4,404,546
rlmC
NOLOHH_22310: ybjO - Inner membrane protein YbjO, at 4,404,587 to 4,405,075
ybjO
NOLOHH_22315: potI - putrescine ABC transporter permease PotI, at 4,405,135 to 4,405,980
potI
NOLOHH_22320: potH - putrescine ABC transporter permease PotH, at 4,405,977 to 4,406,930
potH
Position (kb)
4404
4405
4406 Strain fitness (log2 ratio)
-2
-1
0
1 at 4403.838 kb on + strand, within rlmC at 4403.839 kb on - strand, within rlmC at 4403.839 kb on - strand, within rlmC at 4403.839 kb on - strand, within rlmC at 4403.839 kb on - strand, within rlmC at 4403.883 kb on - strand, within rlmC at 4404.019 kb on - strand, within rlmC at 4404.125 kb on - strand, within rlmC at 4404.125 kb on - strand, within rlmC at 4404.209 kb on + strand, within rlmC at 4404.466 kb on - strand at 4404.547 kb on - strand at 4404.581 kb on + strand at 4404.582 kb on - strand at 4404.582 kb on - strand at 4404.589 kb on - strand at 4404.786 kb on + strand, within ybjO at 4404.976 kb on + strand, within ybjO at 4404.976 kb on + strand, within ybjO at 4404.976 kb on + strand, within ybjO at 4404.979 kb on + strand, within ybjO at 4405.104 kb on + strand at 4405.104 kb on + strand at 4405.104 kb on + strand at 4405.104 kb on + strand at 4405.105 kb on - strand at 4405.109 kb on - strand at 4405.127 kb on + strand at 4405.128 kb on - strand at 4405.197 kb on - strand at 4405.365 kb on + strand, within potI at 4405.366 kb on - strand, within potI at 4405.366 kb on - strand, within potI at 4405.366 kb on - strand, within potI at 4405.417 kb on - strand, within potI at 4405.417 kb on - strand, within potI at 4405.521 kb on - strand, within potI at 4405.884 kb on - strand, within potI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,403,838 + rlmC NOLOHH_22305 0.37 +0.2 4,403,839 - rlmC NOLOHH_22305 0.37 +1.2 4,403,839 - rlmC NOLOHH_22305 0.37 +0.2 4,403,839 - rlmC NOLOHH_22305 0.37 -0.1 4,403,839 - rlmC NOLOHH_22305 0.37 -0.3 4,403,883 - rlmC NOLOHH_22305 0.41 +0.8 4,404,019 - rlmC NOLOHH_22305 0.53 +0.2 4,404,125 - rlmC NOLOHH_22305 0.63 +1.6 4,404,125 - rlmC NOLOHH_22305 0.63 -2.3 4,404,209 + rlmC NOLOHH_22305 0.70 +1.6 4,404,466 - -1.3 4,404,547 - -2.5 4,404,581 + -0.4 4,404,582 - +1.4 4,404,582 - +1.0 4,404,589 - +0.6 4,404,786 + ybjO NOLOHH_22310 0.41 -2.5 4,404,976 + ybjO NOLOHH_22310 0.80 -1.4 4,404,976 + ybjO NOLOHH_22310 0.80 -1.7 4,404,976 + ybjO NOLOHH_22310 0.80 -0.6 4,404,979 + ybjO NOLOHH_22310 0.80 -1.2 4,405,104 + +0.6 4,405,104 + +0.6 4,405,104 + +1.5 4,405,104 + +1.0 4,405,105 - +0.7 4,405,109 - +0.3 4,405,127 + -0.2 4,405,128 - +0.2 4,405,197 - +0.9 4,405,365 + potI NOLOHH_22315 0.27 -0.1 4,405,366 - potI NOLOHH_22315 0.27 -0.6 4,405,366 - potI NOLOHH_22315 0.27 +0.3 4,405,366 - potI NOLOHH_22315 0.27 +0.1 4,405,417 - potI NOLOHH_22315 0.33 -0.5 4,405,417 - potI NOLOHH_22315 0.33 -2.1 4,405,521 - potI NOLOHH_22315 0.46 -0.4 4,405,884 - potI NOLOHH_22315 0.89 +1.1
Or see this region's nucleotide sequence