Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutB and rutC are separated by 11 nucleotides rutC and rutD are separated by 7 nucleotides rutD and rutE are separated by 9 nucleotides rutE and rutF are separated by 10 nucleotides
NOLOHH_21520: rutB - peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB, at 4,230,950 to 4,231,645
rutB
NOLOHH_21525: rutC - pyrimidine utilization protein C, at 4,231,657 to 4,232,043
rutC
NOLOHH_21530: rutD - pyrimidine utilization protein D, at 4,232,051 to 4,232,851
rutD
NOLOHH_21535: rutE - malonic semialdehyde reductase, at 4,232,861 to 4,233,451
rutE
NOLOHH_21540: rutF - malonic semialdehyde reductase, at 4,233,462 to 4,233,956
rutF
Position (kb)
4232
4233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4231.060 kb on - strand, within rutB at 4231.184 kb on + strand, within rutB at 4231.184 kb on + strand, within rutB at 4231.185 kb on - strand, within rutB at 4231.249 kb on + strand, within rutB at 4231.250 kb on - strand, within rutB at 4231.375 kb on + strand, within rutB at 4231.375 kb on + strand, within rutB at 4231.410 kb on + strand, within rutB at 4231.487 kb on + strand, within rutB at 4231.487 kb on + strand, within rutB at 4231.670 kb on + strand at 4231.675 kb on - strand at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.774 kb on - strand, within rutC at 4231.774 kb on - strand, within rutC at 4231.774 kb on - strand, within rutC at 4232.206 kb on + strand, within rutD at 4232.231 kb on + strand, within rutD at 4232.232 kb on - strand, within rutD at 4232.442 kb on + strand, within rutD at 4232.448 kb on - strand, within rutD at 4232.584 kb on - strand, within rutD at 4232.827 kb on - strand at 4232.827 kb on - strand at 4232.860 kb on + strand at 4232.970 kb on - strand, within rutE at 4232.970 kb on - strand, within rutE at 4233.002 kb on - strand, within rutE at 4233.002 kb on - strand, within rutE at 4233.039 kb on + strand, within rutE at 4233.040 kb on - strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.449 kb on + strand at 4233.450 kb on - strand at 4233.461 kb on + strand at 4233.461 kb on + strand at 4233.461 kb on + strand at 4233.469 kb on + strand at 4233.469 kb on + strand at 4233.470 kb on - strand at 4233.470 kb on - strand at 4233.607 kb on + strand, within rutF at 4233.678 kb on - strand, within rutF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,231,060 - rutB NOLOHH_21520 0.16 -0.2 4,231,184 + rutB NOLOHH_21520 0.34 -2.7 4,231,184 + rutB NOLOHH_21520 0.34 -1.6 4,231,185 - rutB NOLOHH_21520 0.34 +0.6 4,231,249 + rutB NOLOHH_21520 0.43 -0.2 4,231,250 - rutB NOLOHH_21520 0.43 -1.4 4,231,375 + rutB NOLOHH_21520 0.61 +0.7 4,231,375 + rutB NOLOHH_21520 0.61 +1.6 4,231,410 + rutB NOLOHH_21520 0.66 -1.8 4,231,487 + rutB NOLOHH_21520 0.77 -0.2 4,231,487 + rutB NOLOHH_21520 0.77 -0.2 4,231,670 + +2.3 4,231,675 - +0.7 4,231,741 + rutC NOLOHH_21525 0.22 +1.3 4,231,741 + rutC NOLOHH_21525 0.22 -0.3 4,231,741 + rutC NOLOHH_21525 0.22 -0.5 4,231,741 + rutC NOLOHH_21525 0.22 +0.6 4,231,741 + rutC NOLOHH_21525 0.22 +0.3 4,231,741 + rutC NOLOHH_21525 0.22 +1.1 4,231,741 + rutC NOLOHH_21525 0.22 -0.4 4,231,741 + rutC NOLOHH_21525 0.22 +0.7 4,231,742 - rutC NOLOHH_21525 0.22 +0.3 4,231,742 - rutC NOLOHH_21525 0.22 +0.8 4,231,742 - rutC NOLOHH_21525 0.22 -0.3 4,231,742 - rutC NOLOHH_21525 0.22 +1.5 4,231,742 - rutC NOLOHH_21525 0.22 +1.1 4,231,742 - rutC NOLOHH_21525 0.22 -0.3 4,231,773 + rutC NOLOHH_21525 0.30 -1.8 4,231,773 + rutC NOLOHH_21525 0.30 +0.2 4,231,773 + rutC NOLOHH_21525 0.30 -0.6 4,231,773 + rutC NOLOHH_21525 0.30 -0.6 4,231,774 - rutC NOLOHH_21525 0.30 -1.5 4,231,774 - rutC NOLOHH_21525 0.30 +0.0 4,231,774 - rutC NOLOHH_21525 0.30 -0.8 4,232,206 + rutD NOLOHH_21530 0.19 +0.8 4,232,231 + rutD NOLOHH_21530 0.22 -1.3 4,232,232 - rutD NOLOHH_21530 0.23 -0.3 4,232,442 + rutD NOLOHH_21530 0.49 +0.4 4,232,448 - rutD NOLOHH_21530 0.50 -0.9 4,232,584 - rutD NOLOHH_21530 0.67 +0.0 4,232,827 - -1.1 4,232,827 - -0.2 4,232,860 + +1.5 4,232,970 - rutE NOLOHH_21535 0.18 +0.9 4,232,970 - rutE NOLOHH_21535 0.18 +0.7 4,233,002 - rutE NOLOHH_21535 0.24 -2.3 4,233,002 - rutE NOLOHH_21535 0.24 -0.5 4,233,039 + rutE NOLOHH_21535 0.30 +0.0 4,233,040 - rutE NOLOHH_21535 0.30 +0.8 4,233,317 + rutE NOLOHH_21535 0.77 -0.2 4,233,317 + rutE NOLOHH_21535 0.77 -1.7 4,233,317 + rutE NOLOHH_21535 0.77 -0.7 4,233,317 + rutE NOLOHH_21535 0.77 +0.2 4,233,318 - rutE NOLOHH_21535 0.77 +0.6 4,233,318 - rutE NOLOHH_21535 0.77 -0.1 4,233,318 - rutE NOLOHH_21535 0.77 +1.2 4,233,318 - rutE NOLOHH_21535 0.77 -2.5 4,233,449 + +0.6 4,233,450 - +1.2 4,233,461 + -0.1 4,233,461 + +1.3 4,233,461 + -0.0 4,233,469 + -0.7 4,233,469 + -0.3 4,233,470 - -0.3 4,233,470 - +0.3 4,233,607 + rutF NOLOHH_21540 0.29 -0.8 4,233,678 - rutF NOLOHH_21540 0.44 +1.7
Or see this region's nucleotide sequence