Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20335

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpA overlap by 1 nucleotidestrpA and yciG are separated by 380 nucleotidesyciG and yciF are separated by 85 nucleotides NOLOHH_20330: trpB - tryptophan synthase subunit beta, at 4,003,389 to 4,004,582 trpB NOLOHH_20335: trpA - tryptophan synthase subunit alpha, at 4,004,582 to 4,005,388 trpA NOLOHH_20340: yciG - Uncharacterized protein YciG, at 4,005,769 to 4,005,948 yciG NOLOHH_20345: yciF - Protein YciF, at 4,006,034 to 4,006,534 yciF Position (kb) 4004 4005 4006Strain fitness (log2 ratio) -3 -2 -1 0 1at 4003.608 kb on + strand, within trpBat 4003.647 kb on - strand, within trpBat 4003.694 kb on + strand, within trpBat 4003.785 kb on + strand, within trpBat 4003.785 kb on + strand, within trpBat 4003.786 kb on - strand, within trpBat 4003.805 kb on - strand, within trpBat 4003.944 kb on + strand, within trpBat 4003.945 kb on - strand, within trpBat 4003.945 kb on - strand, within trpBat 4003.946 kb on + strand, within trpBat 4003.947 kb on - strand, within trpBat 4004.359 kb on - strand, within trpBat 4004.359 kb on - strand, within trpBat 4004.366 kb on + strand, within trpBat 4004.500 kb on + strandat 4004.500 kb on + strandat 4004.500 kb on + strandat 4004.885 kb on + strand, within trpAat 4004.886 kb on - strand, within trpAat 4005.032 kb on - strand, within trpAat 4005.104 kb on + strand, within trpAat 4005.104 kb on + strand, within trpAat 4005.104 kb on + strand, within trpAat 4005.105 kb on - strand, within trpAat 4005.188 kb on + strand, within trpAat 4005.188 kb on + strand, within trpAat 4005.221 kb on - strand, within trpAat 4005.465 kb on - strandat 4005.622 kb on - strandat 4006.086 kb on + strand, within yciF

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,003,608 + trpB NOLOHH_20330 0.18 -1.6
4,003,647 - trpB NOLOHH_20330 0.22 -2.5
4,003,694 + trpB NOLOHH_20330 0.26 -1.2
4,003,785 + trpB NOLOHH_20330 0.33 -0.2
4,003,785 + trpB NOLOHH_20330 0.33 -0.6
4,003,786 - trpB NOLOHH_20330 0.33 -3.3
4,003,805 - trpB NOLOHH_20330 0.35 -3.3
4,003,944 + trpB NOLOHH_20330 0.46 +0.9
4,003,945 - trpB NOLOHH_20330 0.47 -3.4
4,003,945 - trpB NOLOHH_20330 0.47 -2.2
4,003,946 + trpB NOLOHH_20330 0.47 -0.6
4,003,947 - trpB NOLOHH_20330 0.47 -0.0
4,004,359 - trpB NOLOHH_20330 0.81 -3.1
4,004,359 - trpB NOLOHH_20330 0.81 -3.2
4,004,366 + trpB NOLOHH_20330 0.82 -1.5
4,004,500 + -2.8
4,004,500 + -1.0
4,004,500 + -0.9
4,004,885 + trpA NOLOHH_20335 0.38 -2.3
4,004,886 - trpA NOLOHH_20335 0.38 -1.8
4,005,032 - trpA NOLOHH_20335 0.56 -1.9
4,005,104 + trpA NOLOHH_20335 0.65 -1.7
4,005,104 + trpA NOLOHH_20335 0.65 -1.2
4,005,104 + trpA NOLOHH_20335 0.65 -0.7
4,005,105 - trpA NOLOHH_20335 0.65 -2.6
4,005,188 + trpA NOLOHH_20335 0.75 -1.1
4,005,188 + trpA NOLOHH_20335 0.75 -2.3
4,005,221 - trpA NOLOHH_20335 0.79 -1.5
4,005,465 - +0.8
4,005,622 - +0.4
4,006,086 + yciF NOLOHH_20345 0.10 -0.4

Or see this region's nucleotide sequence