Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpB are separated by 11 nucleotides trpB and trpA overlap by 1 nucleotides
NOLOHH_20325: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 4,002,019 to 4,003,377
trpD
NOLOHH_20330: trpB - tryptophan synthase subunit beta, at 4,003,389 to 4,004,582
trpB
NOLOHH_20335: trpA - tryptophan synthase subunit alpha, at 4,004,582 to 4,005,388
trpA
Position (kb)
4003
4004
4005 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4002.422 kb on + strand, within trpD at 4002.513 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.676 kb on + strand, within trpD at 4002.677 kb on - strand, within trpD at 4002.789 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.832 kb on + strand, within trpD at 4002.937 kb on + strand, within trpD at 4002.987 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.140 kb on + strand, within trpD at 4003.140 kb on + strand, within trpD at 4003.141 kb on - strand, within trpD at 4003.144 kb on + strand, within trpD at 4003.152 kb on - strand, within trpD at 4003.196 kb on + strand, within trpD at 4003.199 kb on + strand, within trpD at 4003.251 kb on + strand at 4003.296 kb on + strand at 4003.375 kb on + strand at 4003.375 kb on + strand at 4003.376 kb on - strand at 4003.399 kb on + strand at 4003.410 kb on + strand at 4003.410 kb on + strand at 4003.435 kb on - strand at 4003.435 kb on - strand at 4003.608 kb on + strand, within trpB at 4003.647 kb on - strand, within trpB at 4003.694 kb on + strand, within trpB at 4003.785 kb on + strand, within trpB at 4003.785 kb on + strand, within trpB at 4003.786 kb on - strand, within trpB at 4003.805 kb on - strand, within trpB at 4003.944 kb on + strand, within trpB at 4003.945 kb on - strand, within trpB at 4003.945 kb on - strand, within trpB at 4003.946 kb on + strand, within trpB at 4003.947 kb on - strand, within trpB at 4004.359 kb on - strand, within trpB at 4004.359 kb on - strand, within trpB at 4004.366 kb on + strand, within trpB at 4004.500 kb on + strand at 4004.500 kb on + strand at 4004.500 kb on + strand at 4004.885 kb on + strand, within trpA at 4004.886 kb on - strand, within trpA at 4005.032 kb on - strand, within trpA at 4005.104 kb on + strand, within trpA at 4005.104 kb on + strand, within trpA at 4005.104 kb on + strand, within trpA at 4005.105 kb on - strand, within trpA at 4005.188 kb on + strand, within trpA at 4005.188 kb on + strand, within trpA at 4005.221 kb on - strand, within trpA at 4005.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,002,422 + trpD NOLOHH_20325 0.30 -3.0 4,002,513 + trpD NOLOHH_20325 0.36 -1.4 4,002,672 + trpD NOLOHH_20325 0.48 -5.1 4,002,672 + trpD NOLOHH_20325 0.48 -0.9 4,002,672 + trpD NOLOHH_20325 0.48 -1.9 4,002,672 + trpD NOLOHH_20325 0.48 -3.0 4,002,672 + trpD NOLOHH_20325 0.48 +0.2 4,002,676 + trpD NOLOHH_20325 0.48 -2.7 4,002,677 - trpD NOLOHH_20325 0.48 -1.2 4,002,789 + trpD NOLOHH_20325 0.57 -1.5 4,002,794 + trpD NOLOHH_20325 0.57 -2.0 4,002,794 + trpD NOLOHH_20325 0.57 -3.7 4,002,832 + trpD NOLOHH_20325 0.60 -1.2 4,002,937 + trpD NOLOHH_20325 0.68 -4.7 4,002,987 + trpD NOLOHH_20325 0.71 -0.7 4,003,039 + trpD NOLOHH_20325 0.75 +0.1 4,003,039 + trpD NOLOHH_20325 0.75 -2.7 4,003,039 + trpD NOLOHH_20325 0.75 -2.4 4,003,039 + trpD NOLOHH_20325 0.75 -1.5 4,003,140 + trpD NOLOHH_20325 0.82 -3.1 4,003,140 + trpD NOLOHH_20325 0.82 -3.9 4,003,141 - trpD NOLOHH_20325 0.83 -1.6 4,003,144 + trpD NOLOHH_20325 0.83 -3.5 4,003,152 - trpD NOLOHH_20325 0.83 -1.4 4,003,196 + trpD NOLOHH_20325 0.87 -2.5 4,003,199 + trpD NOLOHH_20325 0.87 -1.3 4,003,251 + -0.9 4,003,296 + -2.3 4,003,375 + +0.6 4,003,375 + -2.0 4,003,376 - -2.2 4,003,399 + -2.5 4,003,410 + -3.1 4,003,410 + -2.8 4,003,435 - -3.1 4,003,435 - -2.7 4,003,608 + trpB NOLOHH_20330 0.18 -1.6 4,003,647 - trpB NOLOHH_20330 0.22 -2.5 4,003,694 + trpB NOLOHH_20330 0.26 -1.2 4,003,785 + trpB NOLOHH_20330 0.33 -0.2 4,003,785 + trpB NOLOHH_20330 0.33 -0.6 4,003,786 - trpB NOLOHH_20330 0.33 -3.3 4,003,805 - trpB NOLOHH_20330 0.35 -3.3 4,003,944 + trpB NOLOHH_20330 0.46 +0.9 4,003,945 - trpB NOLOHH_20330 0.47 -3.4 4,003,945 - trpB NOLOHH_20330 0.47 -2.2 4,003,946 + trpB NOLOHH_20330 0.47 -0.6 4,003,947 - trpB NOLOHH_20330 0.47 -0.0 4,004,359 - trpB NOLOHH_20330 0.81 -3.1 4,004,359 - trpB NOLOHH_20330 0.81 -3.2 4,004,366 + trpB NOLOHH_20330 0.82 -1.5 4,004,500 + -2.8 4,004,500 + -1.0 4,004,500 + -0.9 4,004,885 + trpA NOLOHH_20335 0.38 -2.3 4,004,886 - trpA NOLOHH_20335 0.38 -1.8 4,005,032 - trpA NOLOHH_20335 0.56 -1.9 4,005,104 + trpA NOLOHH_20335 0.65 -1.7 4,005,104 + trpA NOLOHH_20335 0.65 -1.2 4,005,104 + trpA NOLOHH_20335 0.65 -0.7 4,005,105 - trpA NOLOHH_20335 0.65 -2.6 4,005,188 + trpA NOLOHH_20335 0.75 -1.1 4,005,188 + trpA NOLOHH_20335 0.75 -2.3 4,005,221 - trpA NOLOHH_20335 0.79 -1.5 4,005,465 - +0.8
Or see this region's nucleotide sequence