Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpD are separated by 3 nucleotides trpD and trpB are separated by 11 nucleotides
NOLOHH_20320: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 4,000,420 to 4,002,015
trpD
NOLOHH_20325: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 4,002,019 to 4,003,377
trpD
NOLOHH_20330: trpB - tryptophan synthase subunit beta, at 4,003,389 to 4,004,582
trpB
Position (kb)
4002
4003
4004 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4001.042 kb on - strand, within trpD at 4001.042 kb on - strand, within trpD at 4001.277 kb on + strand, within trpD at 4001.277 kb on + strand, within trpD at 4001.284 kb on - strand, within trpD at 4001.361 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.454 kb on - strand, within trpD at 4001.454 kb on - strand, within trpD at 4001.596 kb on + strand, within trpD at 4001.739 kb on + strand, within trpD at 4001.903 kb on + strand at 4001.960 kb on + strand at 4002.013 kb on + strand at 4002.247 kb on + strand, within trpD at 4002.250 kb on - strand, within trpD at 4002.353 kb on + strand, within trpD at 4002.422 kb on + strand, within trpD at 4002.513 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.676 kb on + strand, within trpD at 4002.677 kb on - strand, within trpD at 4002.789 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.832 kb on + strand, within trpD at 4002.937 kb on + strand, within trpD at 4002.987 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.039 kb on + strand, within trpD at 4003.140 kb on + strand, within trpD at 4003.140 kb on + strand, within trpD at 4003.141 kb on - strand, within trpD at 4003.144 kb on + strand, within trpD at 4003.152 kb on - strand, within trpD at 4003.196 kb on + strand, within trpD at 4003.199 kb on + strand, within trpD at 4003.251 kb on + strand at 4003.296 kb on + strand at 4003.375 kb on + strand at 4003.375 kb on + strand at 4003.376 kb on - strand at 4003.399 kb on + strand at 4003.410 kb on + strand at 4003.410 kb on + strand at 4003.435 kb on - strand at 4003.435 kb on - strand at 4003.608 kb on + strand, within trpB at 4003.647 kb on - strand, within trpB at 4003.694 kb on + strand, within trpB at 4003.785 kb on + strand, within trpB at 4003.785 kb on + strand, within trpB at 4003.786 kb on - strand, within trpB at 4003.805 kb on - strand, within trpB at 4003.944 kb on + strand, within trpB at 4003.945 kb on - strand, within trpB at 4003.945 kb on - strand, within trpB at 4003.946 kb on + strand, within trpB at 4003.947 kb on - strand, within trpB at 4004.359 kb on - strand, within trpB at 4004.359 kb on - strand, within trpB at 4004.366 kb on + strand, within trpB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,001,042 - trpD NOLOHH_20320 0.39 -3.6 4,001,042 - trpD NOLOHH_20320 0.39 -2.1 4,001,277 + trpD NOLOHH_20320 0.54 -0.3 4,001,277 + trpD NOLOHH_20320 0.54 -3.2 4,001,284 - trpD NOLOHH_20320 0.54 -2.4 4,001,361 + trpD NOLOHH_20320 0.59 -3.7 4,001,453 + trpD NOLOHH_20320 0.65 -3.3 4,001,453 + trpD NOLOHH_20320 0.65 -1.1 4,001,453 + trpD NOLOHH_20320 0.65 -3.9 4,001,453 + trpD NOLOHH_20320 0.65 +0.7 4,001,453 + trpD NOLOHH_20320 0.65 -1.9 4,001,453 + trpD NOLOHH_20320 0.65 -4.5 4,001,454 - trpD NOLOHH_20320 0.65 -3.5 4,001,454 - trpD NOLOHH_20320 0.65 -2.8 4,001,596 + trpD NOLOHH_20320 0.74 -3.7 4,001,739 + trpD NOLOHH_20320 0.83 -2.7 4,001,903 + -3.2 4,001,960 + -2.5 4,002,013 + -3.2 4,002,247 + trpD NOLOHH_20325 0.17 -1.8 4,002,250 - trpD NOLOHH_20325 0.17 -2.5 4,002,353 + trpD NOLOHH_20325 0.25 -0.7 4,002,422 + trpD NOLOHH_20325 0.30 -3.0 4,002,513 + trpD NOLOHH_20325 0.36 -1.4 4,002,672 + trpD NOLOHH_20325 0.48 -5.1 4,002,672 + trpD NOLOHH_20325 0.48 -0.9 4,002,672 + trpD NOLOHH_20325 0.48 -1.9 4,002,672 + trpD NOLOHH_20325 0.48 -3.0 4,002,672 + trpD NOLOHH_20325 0.48 +0.2 4,002,676 + trpD NOLOHH_20325 0.48 -2.7 4,002,677 - trpD NOLOHH_20325 0.48 -1.2 4,002,789 + trpD NOLOHH_20325 0.57 -1.5 4,002,794 + trpD NOLOHH_20325 0.57 -2.0 4,002,794 + trpD NOLOHH_20325 0.57 -3.7 4,002,832 + trpD NOLOHH_20325 0.60 -1.2 4,002,937 + trpD NOLOHH_20325 0.68 -4.7 4,002,987 + trpD NOLOHH_20325 0.71 -0.7 4,003,039 + trpD NOLOHH_20325 0.75 +0.1 4,003,039 + trpD NOLOHH_20325 0.75 -2.7 4,003,039 + trpD NOLOHH_20325 0.75 -2.4 4,003,039 + trpD NOLOHH_20325 0.75 -1.5 4,003,140 + trpD NOLOHH_20325 0.82 -3.1 4,003,140 + trpD NOLOHH_20325 0.82 -3.9 4,003,141 - trpD NOLOHH_20325 0.83 -1.6 4,003,144 + trpD NOLOHH_20325 0.83 -3.5 4,003,152 - trpD NOLOHH_20325 0.83 -1.4 4,003,196 + trpD NOLOHH_20325 0.87 -2.5 4,003,199 + trpD NOLOHH_20325 0.87 -1.3 4,003,251 + -0.9 4,003,296 + -2.3 4,003,375 + +0.6 4,003,375 + -2.0 4,003,376 - -2.2 4,003,399 + -2.5 4,003,410 + -3.1 4,003,410 + -2.8 4,003,435 - -3.1 4,003,435 - -2.7 4,003,608 + trpB NOLOHH_20330 0.18 -1.6 4,003,647 - trpB NOLOHH_20330 0.22 -2.5 4,003,694 + trpB NOLOHH_20330 0.26 -1.2 4,003,785 + trpB NOLOHH_20330 0.33 -0.2 4,003,785 + trpB NOLOHH_20330 0.33 -0.6 4,003,786 - trpB NOLOHH_20330 0.33 -3.3 4,003,805 - trpB NOLOHH_20330 0.35 -3.3 4,003,944 + trpB NOLOHH_20330 0.46 +0.9 4,003,945 - trpB NOLOHH_20330 0.47 -3.4 4,003,945 - trpB NOLOHH_20330 0.47 -2.2 4,003,946 + trpB NOLOHH_20330 0.47 -0.6 4,003,947 - trpB NOLOHH_20330 0.47 -0.0 4,004,359 - trpB NOLOHH_20330 0.81 -3.1 4,004,359 - trpB NOLOHH_20330 0.81 -3.2 4,004,366 + trpB NOLOHH_20330 0.82 -1.5
Or see this region's nucleotide sequence