Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpD overlap by 1 nucleotides trpD and trpD are separated by 3 nucleotides
NOLOHH_20315: trpE - anthranilate synthase component I, at 3,998,858 to 4,000,420
trpE
NOLOHH_20320: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 4,000,420 to 4,002,015
trpD
NOLOHH_20325: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 4,002,019 to 4,003,377
trpD
Position (kb)
4000
4001
4002
4003 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3999.501 kb on + strand, within trpE at 3999.571 kb on + strand, within trpE at 3999.701 kb on + strand, within trpE at 3999.701 kb on + strand, within trpE at 3999.701 kb on + strand, within trpE at 3999.732 kb on - strand, within trpE at 3999.926 kb on - strand, within trpE at 3999.926 kb on - strand, within trpE at 3999.967 kb on + strand, within trpE at 4000.040 kb on + strand, within trpE at 4000.040 kb on + strand, within trpE at 4000.041 kb on - strand, within trpE at 4000.117 kb on + strand, within trpE at 4000.123 kb on + strand, within trpE at 4000.134 kb on + strand, within trpE at 4000.134 kb on + strand, within trpE at 4000.134 kb on + strand, within trpE at 4000.134 kb on + strand, within trpE at 4000.135 kb on - strand, within trpE at 4000.152 kb on + strand, within trpE at 4000.220 kb on + strand, within trpE at 4000.358 kb on - strand at 4000.463 kb on - strand at 4000.497 kb on + strand at 4000.539 kb on - strand at 4000.539 kb on - strand at 4000.617 kb on + strand, within trpD at 4000.709 kb on - strand, within trpD at 4001.042 kb on - strand, within trpD at 4001.042 kb on - strand, within trpD at 4001.277 kb on + strand, within trpD at 4001.277 kb on + strand, within trpD at 4001.284 kb on - strand, within trpD at 4001.361 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.453 kb on + strand, within trpD at 4001.454 kb on - strand, within trpD at 4001.454 kb on - strand, within trpD at 4001.596 kb on + strand, within trpD at 4001.739 kb on + strand, within trpD at 4001.903 kb on + strand at 4001.960 kb on + strand at 4002.013 kb on + strand at 4002.247 kb on + strand, within trpD at 4002.250 kb on - strand, within trpD at 4002.353 kb on + strand, within trpD at 4002.422 kb on + strand, within trpD at 4002.513 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.672 kb on + strand, within trpD at 4002.676 kb on + strand, within trpD at 4002.677 kb on - strand, within trpD at 4002.789 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.794 kb on + strand, within trpD at 4002.832 kb on + strand, within trpD at 4002.937 kb on + strand, within trpD at 4002.987 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,999,501 + trpE NOLOHH_20315 0.41 -2.2 3,999,571 + trpE NOLOHH_20315 0.46 -0.6 3,999,701 + trpE NOLOHH_20315 0.54 -1.1 3,999,701 + trpE NOLOHH_20315 0.54 -1.4 3,999,701 + trpE NOLOHH_20315 0.54 -3.7 3,999,732 - trpE NOLOHH_20315 0.56 -4.3 3,999,926 - trpE NOLOHH_20315 0.68 -2.1 3,999,926 - trpE NOLOHH_20315 0.68 -0.4 3,999,967 + trpE NOLOHH_20315 0.71 -1.4 4,000,040 + trpE NOLOHH_20315 0.76 -3.2 4,000,040 + trpE NOLOHH_20315 0.76 -2.2 4,000,041 - trpE NOLOHH_20315 0.76 -2.4 4,000,117 + trpE NOLOHH_20315 0.81 +0.4 4,000,123 + trpE NOLOHH_20315 0.81 -2.5 4,000,134 + trpE NOLOHH_20315 0.82 -0.5 4,000,134 + trpE NOLOHH_20315 0.82 -1.5 4,000,134 + trpE NOLOHH_20315 0.82 -1.6 4,000,134 + trpE NOLOHH_20315 0.82 -3.3 4,000,135 - trpE NOLOHH_20315 0.82 -3.8 4,000,152 + trpE NOLOHH_20315 0.83 -4.4 4,000,220 + trpE NOLOHH_20315 0.87 -2.8 4,000,358 - -2.3 4,000,463 - -2.4 4,000,497 + -3.8 4,000,539 - -0.2 4,000,539 - -4.5 4,000,617 + trpD NOLOHH_20320 0.12 -3.6 4,000,709 - trpD NOLOHH_20320 0.18 -0.7 4,001,042 - trpD NOLOHH_20320 0.39 -3.6 4,001,042 - trpD NOLOHH_20320 0.39 -2.1 4,001,277 + trpD NOLOHH_20320 0.54 -0.3 4,001,277 + trpD NOLOHH_20320 0.54 -3.2 4,001,284 - trpD NOLOHH_20320 0.54 -2.4 4,001,361 + trpD NOLOHH_20320 0.59 -3.7 4,001,453 + trpD NOLOHH_20320 0.65 -3.3 4,001,453 + trpD NOLOHH_20320 0.65 -1.1 4,001,453 + trpD NOLOHH_20320 0.65 -3.9 4,001,453 + trpD NOLOHH_20320 0.65 +0.7 4,001,453 + trpD NOLOHH_20320 0.65 -1.9 4,001,453 + trpD NOLOHH_20320 0.65 -4.5 4,001,454 - trpD NOLOHH_20320 0.65 -3.5 4,001,454 - trpD NOLOHH_20320 0.65 -2.8 4,001,596 + trpD NOLOHH_20320 0.74 -3.7 4,001,739 + trpD NOLOHH_20320 0.83 -2.7 4,001,903 + -3.2 4,001,960 + -2.5 4,002,013 + -3.2 4,002,247 + trpD NOLOHH_20325 0.17 -1.8 4,002,250 - trpD NOLOHH_20325 0.17 -2.5 4,002,353 + trpD NOLOHH_20325 0.25 -0.7 4,002,422 + trpD NOLOHH_20325 0.30 -3.0 4,002,513 + trpD NOLOHH_20325 0.36 -1.4 4,002,672 + trpD NOLOHH_20325 0.48 -5.1 4,002,672 + trpD NOLOHH_20325 0.48 -0.9 4,002,672 + trpD NOLOHH_20325 0.48 -1.9 4,002,672 + trpD NOLOHH_20325 0.48 -3.0 4,002,672 + trpD NOLOHH_20325 0.48 +0.2 4,002,676 + trpD NOLOHH_20325 0.48 -2.7 4,002,677 - trpD NOLOHH_20325 0.48 -1.2 4,002,789 + trpD NOLOHH_20325 0.57 -1.5 4,002,794 + trpD NOLOHH_20325 0.57 -2.0 4,002,794 + trpD NOLOHH_20325 0.57 -3.7 4,002,832 + trpD NOLOHH_20325 0.60 -1.2 4,002,937 + trpD NOLOHH_20325 0.68 -4.7 4,002,987 + trpD NOLOHH_20325 0.71 -0.7
Or see this region's nucleotide sequence