Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyk and hexR are separated by 127 nucleotides hexR and zwf are separated by 337 nucleotides
NOLOHH_16835: pyk - pyruvate kinase, at 3,309,597 to 3,311,039
pyk
NOLOHH_16840: hexR - DNA-binding transcriptional regulator HexR, at 3,311,167 to 3,312,036
hexR
NOLOHH_16845: zwf - glucose-6-phosphate dehydrogenase, at 3,312,374 to 3,313,849
zwf
Position (kb)
3311
3312
3313 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3310.167 kb on - strand, within pyk at 3310.424 kb on - strand, within pyk at 3310.627 kb on - strand, within pyk at 3310.773 kb on - strand, within pyk at 3310.931 kb on + strand at 3310.932 kb on - strand at 3310.936 kb on - strand at 3310.936 kb on - strand at 3310.936 kb on - strand at 3311.041 kb on + strand at 3311.041 kb on + strand at 3311.041 kb on + strand at 3311.042 kb on - strand at 3311.042 kb on - strand at 3311.042 kb on - strand at 3311.044 kb on + strand at 3311.121 kb on - strand at 3311.139 kb on + strand at 3311.139 kb on + strand at 3311.139 kb on + strand at 3311.139 kb on + strand at 3311.183 kb on - strand at 3311.188 kb on - strand at 3311.188 kb on - strand at 3311.244 kb on + strand at 3311.245 kb on - strand at 3311.296 kb on + strand, within hexR at 3311.296 kb on + strand, within hexR at 3311.297 kb on - strand, within hexR at 3311.297 kb on - strand, within hexR at 3311.335 kb on - strand, within hexR at 3311.336 kb on + strand, within hexR at 3311.337 kb on - strand, within hexR at 3311.337 kb on - strand, within hexR at 3311.337 kb on - strand, within hexR at 3311.518 kb on - strand, within hexR at 3311.518 kb on - strand, within hexR at 3311.527 kb on - strand, within hexR at 3311.578 kb on - strand, within hexR at 3311.593 kb on - strand, within hexR at 3311.593 kb on - strand, within hexR at 3311.593 kb on - strand, within hexR at 3311.799 kb on - strand, within hexR at 3312.166 kb on + strand at 3312.322 kb on + strand at 3312.328 kb on + strand at 3312.328 kb on + strand at 3312.328 kb on + strand at 3312.328 kb on + strand at 3312.328 kb on + strand at 3312.340 kb on + strand at 3312.340 kb on + strand at 3312.906 kb on - strand, within zwf
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,310,167 - pyk NOLOHH_16835 0.40 +0.1 3,310,424 - pyk NOLOHH_16835 0.57 +0.0 3,310,627 - pyk NOLOHH_16835 0.71 -1.2 3,310,773 - pyk NOLOHH_16835 0.81 +0.0 3,310,931 + -0.9 3,310,932 - +1.4 3,310,936 - -2.8 3,310,936 - -0.7 3,310,936 - -0.2 3,311,041 + -2.0 3,311,041 + -0.2 3,311,041 + +1.1 3,311,042 - -2.6 3,311,042 - -1.0 3,311,042 - +1.3 3,311,044 + -0.2 3,311,121 - +0.8 3,311,139 + -2.4 3,311,139 + +0.1 3,311,139 + +2.0 3,311,139 + -2.7 3,311,183 - -2.1 3,311,188 - -0.8 3,311,188 - -0.2 3,311,244 + +0.4 3,311,245 - -1.0 3,311,296 + hexR NOLOHH_16840 0.15 +0.1 3,311,296 + hexR NOLOHH_16840 0.15 +0.9 3,311,297 - hexR NOLOHH_16840 0.15 +0.8 3,311,297 - hexR NOLOHH_16840 0.15 -0.1 3,311,335 - hexR NOLOHH_16840 0.19 -0.5 3,311,336 + hexR NOLOHH_16840 0.19 +0.6 3,311,337 - hexR NOLOHH_16840 0.20 -0.5 3,311,337 - hexR NOLOHH_16840 0.20 -0.2 3,311,337 - hexR NOLOHH_16840 0.20 -0.5 3,311,518 - hexR NOLOHH_16840 0.40 -2.4 3,311,518 - hexR NOLOHH_16840 0.40 +0.1 3,311,527 - hexR NOLOHH_16840 0.41 +0.4 3,311,578 - hexR NOLOHH_16840 0.47 +1.0 3,311,593 - hexR NOLOHH_16840 0.49 +0.3 3,311,593 - hexR NOLOHH_16840 0.49 +0.6 3,311,593 - hexR NOLOHH_16840 0.49 -0.0 3,311,799 - hexR NOLOHH_16840 0.73 -0.4 3,312,166 + -1.2 3,312,322 + -0.4 3,312,328 + -1.2 3,312,328 + +0.1 3,312,328 + +1.0 3,312,328 + +1.1 3,312,328 + -0.2 3,312,340 + -0.1 3,312,340 + +1.3 3,312,906 - zwf NOLOHH_16845 0.36 -1.8
Or see this region's nucleotide sequence