Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt era and recO are separated by 11 nucleotides recO and pdxJ are separated by 11 nucleotides pdxJ and NOLOHH_13105 overlap by 1 nucleotides NOLOHH_13105 and shoB are separated by 419 nucleotides
NOLOHH_13090: era - GTPase Era, at 2,535,193 to 2,536,098
era
NOLOHH_13095: recO - DNA repair protein RecO, at 2,536,110 to 2,536,838
recO
NOLOHH_13100: pdxJ - pyridoxine 5'-phosphate synthase, at 2,536,850 to 2,537,581
pdxJ
NOLOHH_13105: NOLOHH_13105 - Holo-[acyl-carrier-protein] synthase, at 2,537,581 to 2,537,961
_13105
NOLOHH_13115: shoB - Small toxic protein ShoB, at 2,538,381 to 2,538,461
shoB
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2536.142 kb on + strand at 2536.142 kb on + strand at 2536.241 kb on + strand, within recO at 2536.242 kb on - strand, within recO at 2536.258 kb on + strand, within recO at 2536.258 kb on + strand, within recO at 2536.259 kb on - strand, within recO at 2536.282 kb on + strand, within recO at 2536.359 kb on - strand, within recO at 2536.381 kb on - strand, within recO at 2536.413 kb on + strand, within recO at 2536.600 kb on - strand, within recO at 2536.649 kb on + strand, within recO at 2536.672 kb on + strand, within recO at 2536.672 kb on + strand, within recO at 2536.672 kb on + strand, within recO at 2536.673 kb on - strand, within recO at 2536.755 kb on + strand, within recO at 2536.774 kb on + strand at 2536.986 kb on + strand, within pdxJ at 2536.986 kb on + strand, within pdxJ at 2536.986 kb on + strand, within pdxJ at 2538.000 kb on + strand at 2538.001 kb on - strand at 2538.027 kb on - strand at 2538.184 kb on + strand at 2538.306 kb on + strand at 2538.335 kb on + strand at 2538.335 kb on + strand at 2538.335 kb on + strand at 2538.335 kb on + strand at 2538.336 kb on - strand at 2538.336 kb on - strand at 2538.336 kb on - strand at 2538.343 kb on - strand at 2538.436 kb on + strand, within shoB at 2538.459 kb on + strand at 2538.475 kb on - strand at 2538.478 kb on + strand at 2538.543 kb on - strand at 2538.546 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,536,142 + -1.0 2,536,142 + -0.8 2,536,241 + recO NOLOHH_13095 0.18 +1.0 2,536,242 - recO NOLOHH_13095 0.18 +0.7 2,536,258 + recO NOLOHH_13095 0.20 -1.0 2,536,258 + recO NOLOHH_13095 0.20 +0.4 2,536,259 - recO NOLOHH_13095 0.20 +0.7 2,536,282 + recO NOLOHH_13095 0.24 -2.8 2,536,359 - recO NOLOHH_13095 0.34 -0.7 2,536,381 - recO NOLOHH_13095 0.37 -0.4 2,536,413 + recO NOLOHH_13095 0.42 -0.3 2,536,600 - recO NOLOHH_13095 0.67 +0.8 2,536,649 + recO NOLOHH_13095 0.74 +1.1 2,536,672 + recO NOLOHH_13095 0.77 -1.0 2,536,672 + recO NOLOHH_13095 0.77 +1.0 2,536,672 + recO NOLOHH_13095 0.77 +0.1 2,536,673 - recO NOLOHH_13095 0.77 -0.8 2,536,755 + recO NOLOHH_13095 0.88 +1.0 2,536,774 + -0.6 2,536,986 + pdxJ NOLOHH_13100 0.19 -4.3 2,536,986 + pdxJ NOLOHH_13100 0.19 -1.7 2,536,986 + pdxJ NOLOHH_13100 0.19 -3.7 2,538,000 + -0.5 2,538,001 - +0.6 2,538,027 - -0.4 2,538,184 + -1.2 2,538,306 + -1.0 2,538,335 + -1.2 2,538,335 + +1.0 2,538,335 + +0.1 2,538,335 + -1.1 2,538,336 - +0.6 2,538,336 - +0.0 2,538,336 - -1.0 2,538,343 - +0.2 2,538,436 + shoB NOLOHH_13115 0.68 +0.7 2,538,459 + -1.6 2,538,475 - -2.9 2,538,478 + +0.4 2,538,543 - +0.3 2,538,546 - +1.1
Or see this region's nucleotide sequence