Strain Fitness in Escherichia coli ECOR27 around NOLOHH_13100

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntera and recO are separated by 11 nucleotidesrecO and pdxJ are separated by 11 nucleotidespdxJ and NOLOHH_13105 overlap by 1 nucleotidesNOLOHH_13105 and shoB are separated by 419 nucleotides NOLOHH_13090: era - GTPase Era, at 2,535,193 to 2,536,098 era NOLOHH_13095: recO - DNA repair protein RecO, at 2,536,110 to 2,536,838 recO NOLOHH_13100: pdxJ - pyridoxine 5'-phosphate synthase, at 2,536,850 to 2,537,581 pdxJ NOLOHH_13105: NOLOHH_13105 - Holo-[acyl-carrier-protein] synthase, at 2,537,581 to 2,537,961 _13105 NOLOHH_13115: shoB - Small toxic protein ShoB, at 2,538,381 to 2,538,461 shoB Position (kb) 2536 2537 2538Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2536.142 kb on + strandat 2536.142 kb on + strandat 2536.241 kb on + strand, within recOat 2536.242 kb on - strand, within recOat 2536.258 kb on + strand, within recOat 2536.258 kb on + strand, within recOat 2536.259 kb on - strand, within recOat 2536.282 kb on + strand, within recOat 2536.359 kb on - strand, within recOat 2536.381 kb on - strand, within recOat 2536.413 kb on + strand, within recOat 2536.600 kb on - strand, within recOat 2536.649 kb on + strand, within recOat 2536.672 kb on + strand, within recOat 2536.672 kb on + strand, within recOat 2536.672 kb on + strand, within recOat 2536.673 kb on - strand, within recOat 2536.755 kb on + strand, within recOat 2536.774 kb on + strandat 2536.986 kb on + strand, within pdxJat 2536.986 kb on + strand, within pdxJat 2536.986 kb on + strand, within pdxJat 2538.000 kb on + strandat 2538.001 kb on - strandat 2538.027 kb on - strandat 2538.184 kb on + strandat 2538.306 kb on + strandat 2538.335 kb on + strandat 2538.335 kb on + strandat 2538.335 kb on + strandat 2538.335 kb on + strandat 2538.336 kb on - strandat 2538.336 kb on - strandat 2538.336 kb on - strandat 2538.343 kb on - strandat 2538.436 kb on + strand, within shoBat 2538.459 kb on + strandat 2538.475 kb on - strandat 2538.478 kb on + strandat 2538.543 kb on - strandat 2538.546 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,536,142 + -1.0
2,536,142 + -0.8
2,536,241 + recO NOLOHH_13095 0.18 +1.0
2,536,242 - recO NOLOHH_13095 0.18 +0.7
2,536,258 + recO NOLOHH_13095 0.20 -1.0
2,536,258 + recO NOLOHH_13095 0.20 +0.4
2,536,259 - recO NOLOHH_13095 0.20 +0.7
2,536,282 + recO NOLOHH_13095 0.24 -2.8
2,536,359 - recO NOLOHH_13095 0.34 -0.7
2,536,381 - recO NOLOHH_13095 0.37 -0.4
2,536,413 + recO NOLOHH_13095 0.42 -0.3
2,536,600 - recO NOLOHH_13095 0.67 +0.8
2,536,649 + recO NOLOHH_13095 0.74 +1.1
2,536,672 + recO NOLOHH_13095 0.77 -1.0
2,536,672 + recO NOLOHH_13095 0.77 +1.0
2,536,672 + recO NOLOHH_13095 0.77 +0.1
2,536,673 - recO NOLOHH_13095 0.77 -0.8
2,536,755 + recO NOLOHH_13095 0.88 +1.0
2,536,774 + -0.6
2,536,986 + pdxJ NOLOHH_13100 0.19 -4.3
2,536,986 + pdxJ NOLOHH_13100 0.19 -1.7
2,536,986 + pdxJ NOLOHH_13100 0.19 -3.7
2,538,000 + -0.5
2,538,001 - +0.6
2,538,027 - -0.4
2,538,184 + -1.2
2,538,306 + -1.0
2,538,335 + -1.2
2,538,335 + +1.0
2,538,335 + +0.1
2,538,335 + -1.1
2,538,336 - +0.6
2,538,336 - +0.0
2,538,336 - -1.0
2,538,343 - +0.2
2,538,436 + shoB NOLOHH_13115 0.68 +0.7
2,538,459 + -1.6
2,538,475 - -2.9
2,538,478 + +0.4
2,538,543 - +0.3
2,538,546 - +1.1

Or see this region's nucleotide sequence