Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rseB and rseC overlap by 4 nucleotides rseC and lepA are separated by 197 nucleotides lepA and lepB are separated by 15 nucleotides
NOLOHH_13060: rseB - sigma-E factor regulatory protein RseB, at 2,529,824 to 2,530,780
rseB
NOLOHH_13070: rseC - SoxR-reducing system protein RseC, at 2,530,777 to 2,531,256
rseC
NOLOHH_13075: lepA - translation elongation factor 4, at 2,531,454 to 2,533,253
lepA
NOLOHH_13080: lepB - signal peptidase I, at 2,533,269 to 2,534,243
lepB
Position (kb)
2531
2532
2533
2534 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2530.464 kb on + strand, within rseB at 2530.464 kb on + strand, within rseB at 2530.477 kb on + strand, within rseB at 2530.678 kb on + strand, within rseB at 2530.679 kb on - strand, within rseB at 2530.686 kb on + strand at 2530.686 kb on + strand at 2530.686 kb on + strand at 2530.686 kb on + strand at 2530.756 kb on + strand at 2530.756 kb on + strand at 2530.843 kb on - strand, within rseC at 2531.001 kb on + strand, within rseC at 2531.020 kb on + strand, within rseC at 2531.020 kb on + strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.021 kb on - strand, within rseC at 2531.022 kb on + strand, within rseC at 2531.022 kb on + strand, within rseC at 2531.022 kb on + strand, within rseC at 2531.023 kb on - strand, within rseC at 2531.023 kb on - strand, within rseC at 2531.096 kb on + strand, within rseC at 2531.096 kb on + strand, within rseC at 2531.096 kb on + strand, within rseC at 2531.193 kb on - strand, within rseC at 2531.353 kb on - strand at 2531.357 kb on + strand at 2531.357 kb on + strand at 2531.358 kb on - strand at 2531.376 kb on + strand at 2531.379 kb on + strand at 2531.379 kb on + strand at 2531.379 kb on + strand at 2531.409 kb on + strand at 2531.442 kb on + strand at 2531.442 kb on + strand at 2531.442 kb on + strand at 2532.080 kb on + strand, within lepA at 2532.080 kb on + strand, within lepA at 2532.089 kb on + strand, within lepA at 2532.147 kb on + strand, within lepA at 2532.147 kb on + strand, within lepA at 2532.147 kb on + strand, within lepA at 2532.147 kb on + strand, within lepA at 2532.339 kb on + strand, within lepA at 2532.429 kb on + strand, within lepA at 2532.635 kb on + strand, within lepA at 2532.635 kb on + strand, within lepA at 2532.861 kb on + strand, within lepA at 2532.895 kb on + strand, within lepA at 2532.895 kb on + strand, within lepA at 2532.901 kb on + strand, within lepA at 2532.910 kb on + strand, within lepA at 2532.910 kb on + strand, within lepA at 2533.106 kb on + strand at 2534.242 kb on - strand at 2534.244 kb on + strand at 2534.245 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,530,464 + rseB NOLOHH_13060 0.67 -1.6 2,530,464 + rseB NOLOHH_13060 0.67 -0.9 2,530,477 + rseB NOLOHH_13060 0.68 +0.4 2,530,678 + rseB NOLOHH_13060 0.89 -1.7 2,530,679 - rseB NOLOHH_13060 0.89 +0.1 2,530,686 + +0.2 2,530,686 + -2.9 2,530,686 + +0.4 2,530,686 + -0.3 2,530,756 + -1.4 2,530,756 + -0.6 2,530,843 - rseC NOLOHH_13070 0.14 -0.6 2,531,001 + rseC NOLOHH_13070 0.47 -1.1 2,531,020 + rseC NOLOHH_13070 0.51 +1.9 2,531,020 + rseC NOLOHH_13070 0.51 -0.8 2,531,021 - rseC NOLOHH_13070 0.51 -2.5 2,531,021 - rseC NOLOHH_13070 0.51 -3.0 2,531,021 - rseC NOLOHH_13070 0.51 -0.4 2,531,021 - rseC NOLOHH_13070 0.51 -2.0 2,531,021 - rseC NOLOHH_13070 0.51 +1.1 2,531,021 - rseC NOLOHH_13070 0.51 -2.7 2,531,022 + rseC NOLOHH_13070 0.51 -0.6 2,531,022 + rseC NOLOHH_13070 0.51 -0.8 2,531,022 + rseC NOLOHH_13070 0.51 -1.8 2,531,023 - rseC NOLOHH_13070 0.51 -0.5 2,531,023 - rseC NOLOHH_13070 0.51 -2.9 2,531,096 + rseC NOLOHH_13070 0.66 -0.2 2,531,096 + rseC NOLOHH_13070 0.66 -1.0 2,531,096 + rseC NOLOHH_13070 0.66 +0.1 2,531,193 - rseC NOLOHH_13070 0.87 -2.1 2,531,353 - +0.6 2,531,357 + +0.3 2,531,357 + +0.9 2,531,358 - -1.8 2,531,376 + +0.6 2,531,379 + +0.2 2,531,379 + -0.3 2,531,379 + +0.0 2,531,409 + -0.3 2,531,442 + -1.8 2,531,442 + -0.2 2,531,442 + -0.9 2,532,080 + lepA NOLOHH_13075 0.35 -1.0 2,532,080 + lepA NOLOHH_13075 0.35 -2.1 2,532,089 + lepA NOLOHH_13075 0.35 -1.5 2,532,147 + lepA NOLOHH_13075 0.39 +1.3 2,532,147 + lepA NOLOHH_13075 0.39 -1.2 2,532,147 + lepA NOLOHH_13075 0.39 +0.8 2,532,147 + lepA NOLOHH_13075 0.39 +0.1 2,532,339 + lepA NOLOHH_13075 0.49 -1.5 2,532,429 + lepA NOLOHH_13075 0.54 -1.6 2,532,635 + lepA NOLOHH_13075 0.66 +0.8 2,532,635 + lepA NOLOHH_13075 0.66 -2.4 2,532,861 + lepA NOLOHH_13075 0.78 -3.0 2,532,895 + lepA NOLOHH_13075 0.80 -1.8 2,532,895 + lepA NOLOHH_13075 0.80 -0.4 2,532,901 + lepA NOLOHH_13075 0.80 +0.2 2,532,910 + lepA NOLOHH_13075 0.81 -3.5 2,532,910 + lepA NOLOHH_13075 0.81 -0.1 2,533,106 + -2.4 2,534,242 - +0.7 2,534,244 + -0.2 2,534,245 - -2.0
Or see this region's nucleotide sequence