Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galP and metK are separated by 435 nucleotides metK and speA are separated by 794 nucleotides
NOLOHH_11165: galP - galactose/proton symporter, at 2,159,020 to 2,160,414
galP
NOLOHH_11170: metK - methionine adenosyltransferase, at 2,160,850 to 2,162,004
metK
NOLOHH_11175: speA - biosynthetic arginine decarboxylase, at 2,162,799 to 2,164,775
speA
Position (kb)
2160
2161
2162
2163 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2159.943 kb on - strand, within galP at 2159.978 kb on + strand, within galP at 2160.029 kb on + strand, within galP at 2160.030 kb on - strand, within galP at 2160.044 kb on + strand, within galP at 2160.044 kb on + strand, within galP at 2160.044 kb on + strand, within galP at 2160.094 kb on + strand, within galP at 2160.094 kb on + strand, within galP at 2160.095 kb on - strand, within galP at 2160.184 kb on - strand, within galP at 2160.242 kb on - strand, within galP at 2160.242 kb on - strand, within galP at 2160.242 kb on - strand, within galP at 2160.289 kb on - strand at 2160.310 kb on + strand at 2160.311 kb on - strand at 2160.311 kb on - strand at 2160.427 kb on + strand at 2160.463 kb on + strand at 2160.471 kb on - strand at 2160.560 kb on - strand at 2160.562 kb on - strand at 2160.569 kb on + strand at 2160.569 kb on + strand at 2160.569 kb on + strand at 2160.570 kb on - strand at 2160.575 kb on - strand at 2160.617 kb on + strand at 2160.617 kb on + strand at 2160.618 kb on - strand at 2160.660 kb on + strand at 2160.714 kb on + strand at 2160.740 kb on + strand at 2160.740 kb on + strand at 2160.798 kb on + strand at 2160.851 kb on + strand at 2160.851 kb on + strand at 2162.075 kb on - strand at 2162.254 kb on + strand at 2162.290 kb on + strand at 2162.310 kb on - strand at 2162.313 kb on + strand at 2162.392 kb on - strand at 2162.420 kb on + strand at 2162.421 kb on - strand at 2162.502 kb on + strand at 2162.503 kb on - strand at 2162.503 kb on - strand at 2162.517 kb on + strand at 2162.518 kb on - strand at 2162.555 kb on - strand at 2162.588 kb on + strand at 2162.589 kb on - strand at 2162.631 kb on + strand at 2162.631 kb on + strand at 2162.632 kb on - strand at 2162.722 kb on + strand at 2162.722 kb on + strand at 2162.769 kb on + strand at 2162.770 kb on - strand at 2162.776 kb on - strand at 2162.783 kb on + strand at 2162.798 kb on + strand at 2162.798 kb on + strand at 2162.799 kb on - strand at 2162.816 kb on + strand at 2162.852 kb on - strand at 2162.854 kb on + strand at 2162.854 kb on + strand at 2162.855 kb on - strand at 2162.855 kb on - strand at 2162.855 kb on - strand at 2162.855 kb on - strand at 2162.855 kb on - strand at 2162.909 kb on + strand at 2162.909 kb on + strand at 2162.909 kb on + strand at 2162.909 kb on + strand at 2162.913 kb on + strand at 2162.914 kb on - strand at 2162.914 kb on - strand at 2162.918 kb on + strand at 2162.937 kb on - strand at 2162.940 kb on + strand at 2162.940 kb on + strand at 2162.940 kb on + strand at 2162.940 kb on + strand at 2162.940 kb on + strand at 2162.940 kb on + strand at 2162.941 kb on - strand at 2162.941 kb on - strand at 2162.941 kb on - strand at 2162.943 kb on + strand at 2162.943 kb on + strand at 2162.943 kb on + strand at 2162.943 kb on + strand at 2162.943 kb on + strand at 2162.943 kb on + strand at 2162.944 kb on - strand at 2162.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,159,943 - galP NOLOHH_11165 0.66 -0.4 2,159,978 + galP NOLOHH_11165 0.69 +1.0 2,160,029 + galP NOLOHH_11165 0.72 +1.0 2,160,030 - galP NOLOHH_11165 0.72 +0.2 2,160,044 + galP NOLOHH_11165 0.73 +0.9 2,160,044 + galP NOLOHH_11165 0.73 -2.4 2,160,044 + galP NOLOHH_11165 0.73 +0.5 2,160,094 + galP NOLOHH_11165 0.77 -0.6 2,160,094 + galP NOLOHH_11165 0.77 +0.1 2,160,095 - galP NOLOHH_11165 0.77 -0.4 2,160,184 - galP NOLOHH_11165 0.83 +2.1 2,160,242 - galP NOLOHH_11165 0.88 -0.6 2,160,242 - galP NOLOHH_11165 0.88 -0.4 2,160,242 - galP NOLOHH_11165 0.88 +0.2 2,160,289 - +0.3 2,160,310 + +0.2 2,160,311 - -0.1 2,160,311 - +0.4 2,160,427 + +0.8 2,160,463 + -0.8 2,160,471 - +0.2 2,160,560 - +0.1 2,160,562 - +0.3 2,160,569 + +0.1 2,160,569 + -0.4 2,160,569 + +1.4 2,160,570 - -1.3 2,160,575 - +0.7 2,160,617 + -1.0 2,160,617 + +0.4 2,160,618 - -0.6 2,160,660 + -0.2 2,160,714 + -0.2 2,160,740 + -0.1 2,160,740 + +0.0 2,160,798 + -0.2 2,160,851 + +0.1 2,160,851 + +0.3 2,162,075 - -1.1 2,162,254 + -0.5 2,162,290 + -0.0 2,162,310 - -0.4 2,162,313 + -1.3 2,162,392 - -0.7 2,162,420 + +1.2 2,162,421 - -0.6 2,162,502 + -0.4 2,162,503 - +0.2 2,162,503 - -1.7 2,162,517 + +0.1 2,162,518 - +0.3 2,162,555 - +0.1 2,162,588 + -0.0 2,162,589 - -0.6 2,162,631 + -0.6 2,162,631 + +1.1 2,162,632 - -0.9 2,162,722 + -0.8 2,162,722 + +0.2 2,162,769 + +0.0 2,162,770 - -0.2 2,162,776 - -0.8 2,162,783 + -0.1 2,162,798 + +0.1 2,162,798 + -0.7 2,162,799 - +0.4 2,162,816 + -0.5 2,162,852 - -1.5 2,162,854 + -1.2 2,162,854 + -0.9 2,162,855 - -2.9 2,162,855 - -1.4 2,162,855 - -3.7 2,162,855 - -0.5 2,162,855 - -0.1 2,162,909 + -3.1 2,162,909 + -1.9 2,162,909 + -1.6 2,162,909 + -4.6 2,162,913 + -1.4 2,162,914 - -0.5 2,162,914 - -2.5 2,162,918 + -2.3 2,162,937 - -1.1 2,162,940 + -5.3 2,162,940 + -0.8 2,162,940 + -2.9 2,162,940 + -3.4 2,162,940 + -1.1 2,162,940 + -1.3 2,162,941 - +0.1 2,162,941 - +0.4 2,162,941 - -1.4 2,162,943 + -1.1 2,162,943 + -1.7 2,162,943 + -1.5 2,162,943 + -1.1 2,162,943 + -2.5 2,162,943 + -1.2 2,162,944 - -0.9 2,162,944 - -0.2
Or see this region's nucleotide sequence