Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoA and NOLOHH_09290 are separated by 40 nucleotides NOLOHH_09290 and yhdN are separated by 106 nucleotides yhdN and zntR are separated by 10 nucleotides zntR and arfA are separated by 55 nucleotides arfA and mscL overlap by 4 nucleotides mscL and trkA are separated by 129 nucleotides
NOLOHH_09285: rpoA - DNA-directed RNA polymerase subunit alpha, at 1,791,689 to 1,792,678
rpoA
NOLOHH_09290: NOLOHH_09290 - 50S ribosomal protein L17, at 1,792,719 to 1,793,102
_09290
NOLOHH_09295: yhdN - Uncharacterized protein YhdN, at 1,793,209 to 1,793,577
yhdN
NOLOHH_09300: zntR - Zn(2+)-responsive transcriptional regulator, at 1,793,588 to 1,794,013
zntR
NOLOHH_09305: arfA - alternative ribosome-rescue factor ArfA, at 1,794,069 to 1,794,287
arfA
NOLOHH_09310: mscL - large-conductance mechanosensitive channel protein MscL, at 1,794,284 to 1,794,694
mscL
NOLOHH_09315: trkA - Trk system potassium transporter TrkA, at 1,794,824 to 1,796,200
trkA
Position (kb)
1793
1794
1795 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1793.143 kb on + strand at 1793.144 kb on - strand at 1793.164 kb on + strand at 1793.164 kb on + strand at 1793.164 kb on + strand at 1793.165 kb on - strand at 1793.165 kb on - strand at 1793.169 kb on - strand at 1793.173 kb on + strand at 1793.173 kb on + strand at 1793.208 kb on + strand at 1793.209 kb on - strand at 1793.209 kb on - strand at 1793.209 kb on - strand at 1793.209 kb on - strand at 1793.209 kb on - strand at 1793.209 kb on - strand at 1793.216 kb on + strand at 1793.216 kb on + strand at 1793.216 kb on + strand at 1793.241 kb on + strand at 1793.243 kb on + strand at 1793.243 kb on + strand at 1793.571 kb on + strand at 1793.575 kb on + strand at 1793.575 kb on + strand at 1793.576 kb on - strand at 1793.583 kb on + strand at 1793.583 kb on + strand at 1793.583 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.587 kb on + strand at 1793.588 kb on - strand at 1793.588 kb on - strand at 1793.588 kb on - strand at 1793.591 kb on + strand at 1793.591 kb on + strand at 1793.648 kb on + strand, within zntR at 1793.683 kb on + strand, within zntR at 1793.705 kb on + strand, within zntR at 1793.705 kb on + strand, within zntR at 1793.757 kb on + strand, within zntR at 1793.818 kb on + strand, within zntR at 1793.819 kb on - strand, within zntR at 1793.955 kb on - strand, within zntR at 1794.034 kb on - strand at 1794.052 kb on - strand at 1794.068 kb on + strand at 1794.087 kb on - strand at 1794.087 kb on - strand at 1794.089 kb on + strand at 1794.089 kb on + strand at 1794.089 kb on + strand at 1794.089 kb on + strand at 1794.100 kb on + strand, within arfA at 1794.124 kb on + strand, within arfA at 1794.127 kb on + strand, within arfA at 1794.127 kb on + strand, within arfA at 1794.127 kb on + strand, within arfA at 1794.128 kb on - strand, within arfA at 1794.128 kb on - strand, within arfA at 1794.165 kb on - strand, within arfA at 1794.241 kb on + strand, within arfA at 1794.336 kb on - strand, within mscL at 1794.336 kb on - strand, within mscL at 1794.336 kb on - strand, within mscL at 1794.336 kb on - strand, within mscL at 1794.336 kb on - strand, within mscL at 1794.336 kb on - strand, within mscL at 1794.402 kb on - strand, within mscL at 1794.402 kb on - strand, within mscL at 1794.402 kb on - strand, within mscL at 1794.407 kb on - strand, within mscL at 1794.494 kb on - strand, within mscL at 1794.552 kb on - strand, within mscL at 1794.552 kb on - strand, within mscL at 1794.552 kb on - strand, within mscL at 1794.552 kb on - strand, within mscL at 1794.552 kb on - strand, within mscL at 1794.555 kb on - strand, within mscL at 1794.723 kb on + strand at 1794.724 kb on - strand at 1794.729 kb on + strand at 1794.739 kb on + strand at 1794.795 kb on + strand at 1794.796 kb on - strand at 1794.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,793,143 + +0.8 1,793,144 - +0.9 1,793,164 + +1.0 1,793,164 + -0.2 1,793,164 + +0.3 1,793,165 - -0.7 1,793,165 - +1.0 1,793,169 - +0.4 1,793,173 + -0.1 1,793,173 + +0.4 1,793,208 + +0.7 1,793,209 - +0.2 1,793,209 - +0.9 1,793,209 - +1.1 1,793,209 - -0.6 1,793,209 - -1.1 1,793,209 - -0.6 1,793,216 + +1.0 1,793,216 + -0.3 1,793,216 + +0.2 1,793,241 + +1.0 1,793,243 + +0.9 1,793,243 + +0.2 1,793,571 + -0.8 1,793,575 + -0.3 1,793,575 + +0.4 1,793,576 - -2.4 1,793,583 + +0.0 1,793,583 + +1.2 1,793,583 + +0.8 1,793,587 + -0.9 1,793,587 + -3.8 1,793,587 + -0.5 1,793,587 + -0.1 1,793,587 + -0.0 1,793,587 + +0.4 1,793,587 + -1.0 1,793,587 + -0.4 1,793,587 + -1.4 1,793,587 + -0.7 1,793,588 - +0.2 1,793,588 - +1.2 1,793,588 - -1.1 1,793,591 + +0.7 1,793,591 + +0.7 1,793,648 + zntR NOLOHH_09300 0.14 -0.6 1,793,683 + zntR NOLOHH_09300 0.22 -0.6 1,793,705 + zntR NOLOHH_09300 0.27 -1.1 1,793,705 + zntR NOLOHH_09300 0.27 -0.4 1,793,757 + zntR NOLOHH_09300 0.40 -0.7 1,793,818 + zntR NOLOHH_09300 0.54 -0.2 1,793,819 - zntR NOLOHH_09300 0.54 -0.3 1,793,955 - zntR NOLOHH_09300 0.86 -0.3 1,794,034 - -0.6 1,794,052 - -0.6 1,794,068 + -0.3 1,794,087 - +0.4 1,794,087 - +0.5 1,794,089 + -0.1 1,794,089 + +0.4 1,794,089 + +0.8 1,794,089 + +0.4 1,794,100 + arfA NOLOHH_09305 0.14 -0.5 1,794,124 + arfA NOLOHH_09305 0.25 +1.2 1,794,127 + arfA NOLOHH_09305 0.26 +0.2 1,794,127 + arfA NOLOHH_09305 0.26 +0.8 1,794,127 + arfA NOLOHH_09305 0.26 -0.9 1,794,128 - arfA NOLOHH_09305 0.27 +1.4 1,794,128 - arfA NOLOHH_09305 0.27 +0.5 1,794,165 - arfA NOLOHH_09305 0.44 +0.3 1,794,241 + arfA NOLOHH_09305 0.79 +0.4 1,794,336 - mscL NOLOHH_09310 0.13 +0.1 1,794,336 - mscL NOLOHH_09310 0.13 -0.3 1,794,336 - mscL NOLOHH_09310 0.13 +0.7 1,794,336 - mscL NOLOHH_09310 0.13 +0.0 1,794,336 - mscL NOLOHH_09310 0.13 -1.5 1,794,336 - mscL NOLOHH_09310 0.13 +0.1 1,794,402 - mscL NOLOHH_09310 0.29 -0.0 1,794,402 - mscL NOLOHH_09310 0.29 +0.7 1,794,402 - mscL NOLOHH_09310 0.29 -0.1 1,794,407 - mscL NOLOHH_09310 0.30 -0.3 1,794,494 - mscL NOLOHH_09310 0.51 +0.7 1,794,552 - mscL NOLOHH_09310 0.65 +0.2 1,794,552 - mscL NOLOHH_09310 0.65 +1.0 1,794,552 - mscL NOLOHH_09310 0.65 +0.6 1,794,552 - mscL NOLOHH_09310 0.65 -1.1 1,794,552 - mscL NOLOHH_09310 0.65 +0.5 1,794,555 - mscL NOLOHH_09310 0.66 +2.1 1,794,723 + +0.9 1,794,724 - -0.0 1,794,729 + -0.3 1,794,739 + -2.2 1,794,795 + +1.1 1,794,796 - +0.9 1,794,821 + -1.1
Or see this region's nucleotide sequence