Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gntT and NOLOHH_08775 are separated by 360 nucleotides NOLOHH_08775 and gntX are separated by 58 nucleotides gntX and bioH are separated by 37 nucleotides bioH and rpnA are separated by 28 nucleotides
NOLOHH_08770: gntT - gluconate transporter, at 1,705,128 to 1,706,444
gntT
NOLOHH_08775: NOLOHH_08775 - NfuA family Fe-S biogenesis protein, at 1,706,805 to 1,707,380
_08775
NOLOHH_08780: gntX - DNA utilization protein GntX, at 1,707,439 to 1,708,122
gntX
NOLOHH_08785: bioH - pimeloyl-ACP methyl ester esterase BioH, at 1,708,160 to 1,708,930
bioH
NOLOHH_08790: rpnA - recombination-promoting nuclease RpnA, at 1,708,959 to 1,709,531
rpnA
Position (kb)
1707
1708
1709 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1706.502 kb on - strand at 1706.526 kb on + strand at 1706.546 kb on + strand at 1706.547 kb on - strand at 1706.559 kb on + strand at 1706.560 kb on - strand at 1706.585 kb on + strand at 1706.586 kb on - strand at 1706.660 kb on + strand at 1706.677 kb on - strand at 1706.679 kb on + strand at 1706.748 kb on + strand at 1706.749 kb on - strand at 1706.749 kb on - strand at 1706.768 kb on - strand at 1706.788 kb on - strand at 1706.788 kb on - strand at 1706.806 kb on + strand at 1706.806 kb on + strand at 1706.806 kb on + strand at 1706.807 kb on - strand at 1706.807 kb on - strand at 1706.809 kb on + strand at 1706.810 kb on - strand at 1706.812 kb on + strand at 1706.812 kb on + strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1706.813 kb on - strand at 1707.041 kb on + strand, within NOLOHH_08775 at 1707.042 kb on - strand, within NOLOHH_08775 at 1707.042 kb on - strand, within NOLOHH_08775 at 1707.043 kb on + strand, within NOLOHH_08775 at 1707.043 kb on + strand, within NOLOHH_08775 at 1707.043 kb on + strand, within NOLOHH_08775 at 1707.043 kb on + strand, within NOLOHH_08775 at 1707.044 kb on - strand, within NOLOHH_08775 at 1707.044 kb on - strand, within NOLOHH_08775 at 1707.044 kb on - strand, within NOLOHH_08775 at 1707.084 kb on - strand, within NOLOHH_08775 at 1707.167 kb on - strand, within NOLOHH_08775 at 1707.167 kb on - strand, within NOLOHH_08775 at 1707.190 kb on + strand, within NOLOHH_08775 at 1707.191 kb on - strand, within NOLOHH_08775 at 1707.191 kb on - strand, within NOLOHH_08775 at 1707.253 kb on + strand, within NOLOHH_08775 at 1707.253 kb on + strand, within NOLOHH_08775 at 1707.372 kb on - strand at 1707.380 kb on + strand at 1707.380 kb on + strand at 1707.380 kb on + strand at 1707.381 kb on - strand at 1707.383 kb on + strand at 1707.402 kb on + strand at 1707.417 kb on + strand at 1707.418 kb on - strand at 1707.440 kb on + strand at 1707.441 kb on - strand at 1707.490 kb on + strand at 1707.491 kb on - strand at 1707.491 kb on - strand at 1707.520 kb on - strand, within gntX at 1707.556 kb on - strand, within gntX at 1707.556 kb on - strand, within gntX at 1707.654 kb on + strand, within gntX at 1707.654 kb on + strand, within gntX at 1707.654 kb on + strand, within gntX at 1707.655 kb on - strand, within gntX at 1707.655 kb on - strand, within gntX at 1707.655 kb on - strand, within gntX at 1707.729 kb on + strand, within gntX at 1707.730 kb on - strand, within gntX at 1707.817 kb on + strand, within gntX at 1707.817 kb on + strand, within gntX at 1707.817 kb on + strand, within gntX at 1707.818 kb on - strand, within gntX at 1707.829 kb on + strand, within gntX at 1707.829 kb on + strand, within gntX at 1707.830 kb on - strand, within gntX at 1707.830 kb on - strand, within gntX at 1707.830 kb on - strand, within gntX at 1707.830 kb on - strand, within gntX at 1707.832 kb on + strand, within gntX at 1707.901 kb on + strand, within gntX at 1707.914 kb on + strand, within gntX at 1707.915 kb on - strand, within gntX at 1708.016 kb on - strand, within gntX at 1708.023 kb on + strand, within gntX at 1708.023 kb on + strand, within gntX at 1708.023 kb on + strand, within gntX at 1708.102 kb on + strand at 1708.102 kb on + strand at 1708.102 kb on + strand at 1708.103 kb on - strand at 1708.112 kb on - strand at 1708.117 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.122 kb on + strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.123 kb on - strand at 1708.136 kb on + strand at 1708.137 kb on - strand at 1708.137 kb on - strand at 1708.165 kb on + strand at 1708.165 kb on + strand at 1708.165 kb on + strand at 1708.267 kb on + strand, within bioH at 1708.267 kb on + strand, within bioH at 1708.268 kb on - strand, within bioH at 1708.268 kb on - strand, within bioH at 1708.268 kb on - strand, within bioH at 1708.268 kb on - strand, within bioH at 1708.279 kb on + strand, within bioH at 1708.348 kb on + strand, within bioH at 1708.349 kb on - strand, within bioH at 1708.573 kb on + strand, within bioH at 1708.596 kb on + strand, within bioH at 1708.766 kb on + strand, within bioH at 1708.766 kb on + strand, within bioH at 1708.766 kb on + strand, within bioH at 1708.766 kb on + strand, within bioH at 1708.766 kb on + strand, within bioH at 1708.767 kb on - strand, within bioH at 1708.767 kb on - strand, within bioH at 1708.767 kb on - strand, within bioH at 1708.767 kb on - strand, within bioH at 1708.767 kb on - strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.841 kb on + strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.842 kb on - strand, within bioH at 1708.843 kb on + strand, within bioH at 1708.843 kb on + strand, within bioH at 1708.844 kb on - strand, within bioH at 1708.844 kb on - strand, within bioH at 1708.844 kb on - strand, within bioH at 1708.844 kb on - strand, within bioH at 1708.873 kb on + strand at 1708.873 kb on + strand at 1708.873 kb on + strand at 1708.928 kb on + strand at 1709.043 kb on + strand, within rpnA at 1709.043 kb on + strand, within rpnA at 1709.043 kb on + strand, within rpnA at 1709.043 kb on + strand, within rpnA at 1709.044 kb on - strand, within rpnA at 1709.052 kb on + strand, within rpnA at 1709.054 kb on + strand, within rpnA at 1709.054 kb on + strand, within rpnA at 1709.055 kb on - strand, within rpnA at 1709.055 kb on - strand, within rpnA at 1709.055 kb on - strand, within rpnA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,706,502 - -0.1 1,706,526 + -0.2 1,706,546 + +0.2 1,706,547 - +0.6 1,706,559 + -0.8 1,706,560 - -0.7 1,706,585 + +0.7 1,706,586 - +0.5 1,706,660 + -0.3 1,706,677 - -0.9 1,706,679 + +0.1 1,706,748 + +0.4 1,706,749 - +0.0 1,706,749 - +0.5 1,706,768 - +0.5 1,706,788 - -0.2 1,706,788 - -3.2 1,706,806 + +0.6 1,706,806 + +0.5 1,706,806 + +0.4 1,706,807 - -0.5 1,706,807 - -0.3 1,706,809 + -0.0 1,706,810 - +0.4 1,706,812 + -1.2 1,706,812 + -0.2 1,706,813 - +0.9 1,706,813 - -0.4 1,706,813 - +0.7 1,706,813 - -0.1 1,706,813 - -0.2 1,706,813 - -0.7 1,706,813 - -0.8 1,707,041 + NOLOHH_08775 0.41 -0.0 1,707,042 - NOLOHH_08775 0.41 -3.1 1,707,042 - NOLOHH_08775 0.41 -0.5 1,707,043 + NOLOHH_08775 0.41 -1.6 1,707,043 + NOLOHH_08775 0.41 -2.4 1,707,043 + NOLOHH_08775 0.41 -2.2 1,707,043 + NOLOHH_08775 0.41 -2.3 1,707,044 - NOLOHH_08775 0.41 -0.2 1,707,044 - NOLOHH_08775 0.41 -1.0 1,707,044 - NOLOHH_08775 0.41 -2.7 1,707,084 - NOLOHH_08775 0.48 -1.1 1,707,167 - NOLOHH_08775 0.63 -1.1 1,707,167 - NOLOHH_08775 0.63 -3.5 1,707,190 + NOLOHH_08775 0.67 -1.0 1,707,191 - NOLOHH_08775 0.67 -2.3 1,707,191 - NOLOHH_08775 0.67 -3.1 1,707,253 + NOLOHH_08775 0.78 -2.7 1,707,253 + NOLOHH_08775 0.78 -3.3 1,707,372 - -1.5 1,707,380 + -0.9 1,707,380 + -3.1 1,707,380 + -1.9 1,707,381 - -0.3 1,707,383 + -2.5 1,707,402 + -0.2 1,707,417 + -0.4 1,707,418 - -0.2 1,707,440 + -1.0 1,707,441 - -0.4 1,707,490 + +0.7 1,707,491 - -1.3 1,707,491 - +0.3 1,707,520 - gntX NOLOHH_08780 0.12 -1.1 1,707,556 - gntX NOLOHH_08780 0.17 +0.5 1,707,556 - gntX NOLOHH_08780 0.17 +0.0 1,707,654 + gntX NOLOHH_08780 0.31 +0.2 1,707,654 + gntX NOLOHH_08780 0.31 -0.3 1,707,654 + gntX NOLOHH_08780 0.31 -0.5 1,707,655 - gntX NOLOHH_08780 0.32 -0.1 1,707,655 - gntX NOLOHH_08780 0.32 +0.3 1,707,655 - gntX NOLOHH_08780 0.32 +1.3 1,707,729 + gntX NOLOHH_08780 0.42 -0.5 1,707,730 - gntX NOLOHH_08780 0.43 -0.1 1,707,817 + gntX NOLOHH_08780 0.55 +0.4 1,707,817 + gntX NOLOHH_08780 0.55 +0.2 1,707,817 + gntX NOLOHH_08780 0.55 -0.2 1,707,818 - gntX NOLOHH_08780 0.55 +0.1 1,707,829 + gntX NOLOHH_08780 0.57 +0.3 1,707,829 + gntX NOLOHH_08780 0.57 +0.3 1,707,830 - gntX NOLOHH_08780 0.57 -1.7 1,707,830 - gntX NOLOHH_08780 0.57 +0.4 1,707,830 - gntX NOLOHH_08780 0.57 +1.2 1,707,830 - gntX NOLOHH_08780 0.57 -1.0 1,707,832 + gntX NOLOHH_08780 0.57 +0.2 1,707,901 + gntX NOLOHH_08780 0.68 -0.4 1,707,914 + gntX NOLOHH_08780 0.69 +0.6 1,707,915 - gntX NOLOHH_08780 0.70 +0.5 1,708,016 - gntX NOLOHH_08780 0.84 +0.1 1,708,023 + gntX NOLOHH_08780 0.85 -0.3 1,708,023 + gntX NOLOHH_08780 0.85 -0.6 1,708,023 + gntX NOLOHH_08780 0.85 +0.4 1,708,102 + +1.4 1,708,102 + -0.4 1,708,102 + +0.8 1,708,103 - -0.3 1,708,112 - -0.1 1,708,117 + -0.2 1,708,122 + -0.3 1,708,122 + +0.8 1,708,122 + +0.2 1,708,122 + -0.6 1,708,122 + -0.3 1,708,122 + -0.2 1,708,122 + -0.7 1,708,122 + -0.1 1,708,122 + +0.1 1,708,123 - -0.0 1,708,123 - +0.3 1,708,123 - +0.6 1,708,123 - -0.6 1,708,123 - -0.9 1,708,123 - +0.4 1,708,123 - +0.0 1,708,123 - -3.5 1,708,123 - -0.4 1,708,123 - -0.2 1,708,123 - -0.2 1,708,123 - -2.7 1,708,123 - -1.2 1,708,136 + -0.9 1,708,137 - +0.4 1,708,137 - -0.5 1,708,165 + +0.3 1,708,165 + -0.7 1,708,165 + +0.2 1,708,267 + bioH NOLOHH_08785 0.14 -1.2 1,708,267 + bioH NOLOHH_08785 0.14 -4.2 1,708,268 - bioH NOLOHH_08785 0.14 -0.3 1,708,268 - bioH NOLOHH_08785 0.14 -0.9 1,708,268 - bioH NOLOHH_08785 0.14 -0.2 1,708,268 - bioH NOLOHH_08785 0.14 -0.8 1,708,279 + bioH NOLOHH_08785 0.15 -0.7 1,708,348 + bioH NOLOHH_08785 0.24 -1.2 1,708,349 - bioH NOLOHH_08785 0.25 -1.1 1,708,573 + bioH NOLOHH_08785 0.54 -2.3 1,708,596 + bioH NOLOHH_08785 0.57 -0.4 1,708,766 + bioH NOLOHH_08785 0.79 -1.4 1,708,766 + bioH NOLOHH_08785 0.79 -1.3 1,708,766 + bioH NOLOHH_08785 0.79 -1.4 1,708,766 + bioH NOLOHH_08785 0.79 +0.2 1,708,766 + bioH NOLOHH_08785 0.79 -0.1 1,708,767 - bioH NOLOHH_08785 0.79 -0.0 1,708,767 - bioH NOLOHH_08785 0.79 -1.6 1,708,767 - bioH NOLOHH_08785 0.79 -0.2 1,708,767 - bioH NOLOHH_08785 0.79 -0.3 1,708,767 - bioH NOLOHH_08785 0.79 +0.5 1,708,841 + bioH NOLOHH_08785 0.88 -0.6 1,708,841 + bioH NOLOHH_08785 0.88 +0.5 1,708,841 + bioH NOLOHH_08785 0.88 -1.1 1,708,841 + bioH NOLOHH_08785 0.88 -0.7 1,708,841 + bioH NOLOHH_08785 0.88 -1.6 1,708,841 + bioH NOLOHH_08785 0.88 -0.7 1,708,841 + bioH NOLOHH_08785 0.88 -0.6 1,708,841 + bioH NOLOHH_08785 0.88 -1.3 1,708,842 - bioH NOLOHH_08785 0.88 -1.4 1,708,842 - bioH NOLOHH_08785 0.88 -0.4 1,708,842 - bioH NOLOHH_08785 0.88 -1.1 1,708,842 - bioH NOLOHH_08785 0.88 -1.3 1,708,842 - bioH NOLOHH_08785 0.88 -1.0 1,708,842 - bioH NOLOHH_08785 0.88 -0.8 1,708,842 - bioH NOLOHH_08785 0.88 -0.6 1,708,842 - bioH NOLOHH_08785 0.88 -0.8 1,708,842 - bioH NOLOHH_08785 0.88 -1.4 1,708,843 + bioH NOLOHH_08785 0.89 +0.1 1,708,843 + bioH NOLOHH_08785 0.89 -2.9 1,708,844 - bioH NOLOHH_08785 0.89 -0.9 1,708,844 - bioH NOLOHH_08785 0.89 -0.1 1,708,844 - bioH NOLOHH_08785 0.89 -0.7 1,708,844 - bioH NOLOHH_08785 0.89 -2.3 1,708,873 + -0.9 1,708,873 + -2.6 1,708,873 + -0.6 1,708,928 + +0.2 1,709,043 + rpnA NOLOHH_08790 0.15 +0.4 1,709,043 + rpnA NOLOHH_08790 0.15 -0.2 1,709,043 + rpnA NOLOHH_08790 0.15 -1.0 1,709,043 + rpnA NOLOHH_08790 0.15 +0.2 1,709,044 - rpnA NOLOHH_08790 0.15 -1.2 1,709,052 + rpnA NOLOHH_08790 0.16 -2.4 1,709,054 + rpnA NOLOHH_08790 0.17 +0.0 1,709,054 + rpnA NOLOHH_08790 0.17 -0.4 1,709,055 - rpnA NOLOHH_08790 0.17 -0.6 1,709,055 - rpnA NOLOHH_08790 0.17 +0.2 1,709,055 - rpnA NOLOHH_08790 0.17 +0.1
Or see this region's nucleotide sequence