Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceB and metA are separated by 268 nucleotides metA and yjaB are separated by 156 nucleotides yjaB and 5S_rrna are separated by 114 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides
NOLOHH_05415: aceB - malate synthase A, at 1,003,954 to 1,005,555
aceB
NOLOHH_05420: metA - homoserine O-succinyltransferase, at 1,005,824 to 1,006,753
metA
NOLOHH_05425: yjaB - acetyltransferase, at 1,006,910 to 1,007,353
yjaB
NOLOHH_05430: 5S_rrna - 5S ribosomal RNA, at 1,007,468 to 1,007,583
_rrna
NOLOHH_05435: 23S_rrna - 23S ribosomal RNA, at 1,007,678 to 1,010,581
_rrna
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1004.848 kb on - strand, within aceB at 1004.864 kb on + strand, within aceB at 1004.921 kb on + strand, within aceB at 1004.922 kb on - strand, within aceB at 1004.961 kb on - strand, within aceB at 1004.961 kb on - strand, within aceB at 1004.961 kb on - strand, within aceB at 1004.961 kb on - strand, within aceB at 1004.961 kb on - strand, within aceB at 1004.975 kb on + strand, within aceB at 1004.976 kb on - strand, within aceB at 1005.117 kb on + strand, within aceB at 1005.117 kb on + strand, within aceB at 1005.120 kb on - strand, within aceB at 1005.153 kb on + strand, within aceB at 1005.274 kb on + strand, within aceB at 1005.274 kb on + strand, within aceB at 1005.384 kb on + strand, within aceB at 1005.385 kb on - strand, within aceB at 1005.481 kb on - strand at 1005.514 kb on + strand at 1005.515 kb on - strand at 1005.785 kb on - strand at 1005.852 kb on - strand at 1005.880 kb on - strand at 1005.880 kb on - strand at 1005.882 kb on + strand at 1005.883 kb on - strand at 1005.883 kb on - strand at 1005.883 kb on - strand at 1005.940 kb on + strand, within metA at 1005.941 kb on - strand, within metA at 1005.967 kb on - strand, within metA at 1005.970 kb on + strand, within metA at 1005.972 kb on + strand, within metA at 1005.972 kb on + strand, within metA at 1005.972 kb on + strand, within metA at 1005.977 kb on + strand, within metA at 1006.041 kb on + strand, within metA at 1006.095 kb on + strand, within metA at 1006.096 kb on - strand, within metA at 1006.096 kb on - strand, within metA at 1006.096 kb on - strand, within metA at 1006.140 kb on + strand, within metA at 1006.240 kb on + strand, within metA at 1006.296 kb on + strand, within metA at 1006.483 kb on + strand, within metA at 1006.483 kb on + strand, within metA at 1006.488 kb on + strand, within metA at 1006.488 kb on + strand, within metA at 1006.489 kb on - strand, within metA at 1006.489 kb on - strand, within metA at 1006.645 kb on + strand, within metA at 1006.645 kb on + strand, within metA at 1006.646 kb on - strand, within metA at 1006.874 kb on + strand at 1006.879 kb on + strand at 1006.879 kb on + strand at 1006.884 kb on + strand at 1006.886 kb on + strand at 1006.886 kb on + strand at 1006.887 kb on - strand at 1006.887 kb on - strand at 1006.918 kb on + strand at 1006.918 kb on + strand at 1007.104 kb on + strand, within yjaB at 1007.104 kb on + strand, within yjaB at 1007.112 kb on + strand, within yjaB at 1007.125 kb on + strand, within yjaB at 1007.258 kb on + strand, within yjaB at 1007.258 kb on + strand, within yjaB at 1007.260 kb on + strand, within yjaB at 1007.260 kb on + strand, within yjaB at 1007.339 kb on + strand at 1007.339 kb on + strand at 1007.339 kb on + strand at 1007.339 kb on + strand at 1007.340 kb on - strand at 1007.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,004,848 - aceB NOLOHH_05415 0.56 +0.5 1,004,864 + aceB NOLOHH_05415 0.57 +0.1 1,004,921 + aceB NOLOHH_05415 0.60 -0.1 1,004,922 - aceB NOLOHH_05415 0.60 -0.3 1,004,961 - aceB NOLOHH_05415 0.63 -1.1 1,004,961 - aceB NOLOHH_05415 0.63 +0.6 1,004,961 - aceB NOLOHH_05415 0.63 +0.4 1,004,961 - aceB NOLOHH_05415 0.63 -0.1 1,004,961 - aceB NOLOHH_05415 0.63 +0.6 1,004,975 + aceB NOLOHH_05415 0.64 -1.2 1,004,976 - aceB NOLOHH_05415 0.64 +1.1 1,005,117 + aceB NOLOHH_05415 0.73 +0.9 1,005,117 + aceB NOLOHH_05415 0.73 -0.9 1,005,120 - aceB NOLOHH_05415 0.73 +0.6 1,005,153 + aceB NOLOHH_05415 0.75 +0.4 1,005,274 + aceB NOLOHH_05415 0.82 -0.3 1,005,274 + aceB NOLOHH_05415 0.82 +0.3 1,005,384 + aceB NOLOHH_05415 0.89 -0.6 1,005,385 - aceB NOLOHH_05415 0.89 -0.4 1,005,481 - -0.0 1,005,514 + +0.3 1,005,515 - -1.0 1,005,785 - +0.4 1,005,852 - -0.0 1,005,880 - -0.2 1,005,880 - +0.7 1,005,882 + +0.2 1,005,883 - +0.2 1,005,883 - +1.8 1,005,883 - +0.8 1,005,940 + metA NOLOHH_05420 0.12 +0.5 1,005,941 - metA NOLOHH_05420 0.13 -0.5 1,005,967 - metA NOLOHH_05420 0.15 +1.1 1,005,970 + metA NOLOHH_05420 0.16 -0.8 1,005,972 + metA NOLOHH_05420 0.16 -0.7 1,005,972 + metA NOLOHH_05420 0.16 -0.7 1,005,972 + metA NOLOHH_05420 0.16 +0.0 1,005,977 + metA NOLOHH_05420 0.16 -2.1 1,006,041 + metA NOLOHH_05420 0.23 +0.0 1,006,095 + metA NOLOHH_05420 0.29 +0.2 1,006,096 - metA NOLOHH_05420 0.29 +0.1 1,006,096 - metA NOLOHH_05420 0.29 +0.4 1,006,096 - metA NOLOHH_05420 0.29 -2.2 1,006,140 + metA NOLOHH_05420 0.34 +0.3 1,006,240 + metA NOLOHH_05420 0.45 -0.7 1,006,296 + metA NOLOHH_05420 0.51 +0.2 1,006,483 + metA NOLOHH_05420 0.71 +0.8 1,006,483 + metA NOLOHH_05420 0.71 -0.4 1,006,488 + metA NOLOHH_05420 0.71 +0.2 1,006,488 + metA NOLOHH_05420 0.71 -0.0 1,006,489 - metA NOLOHH_05420 0.72 -0.9 1,006,489 - metA NOLOHH_05420 0.72 +0.5 1,006,645 + metA NOLOHH_05420 0.88 +0.4 1,006,645 + metA NOLOHH_05420 0.88 +0.2 1,006,646 - metA NOLOHH_05420 0.88 +0.7 1,006,874 + +0.5 1,006,879 + -0.3 1,006,879 + -0.3 1,006,884 + +0.9 1,006,886 + -1.4 1,006,886 + +1.0 1,006,887 - +0.7 1,006,887 - -1.7 1,006,918 + +0.2 1,006,918 + -0.2 1,007,104 + yjaB NOLOHH_05425 0.44 +0.8 1,007,104 + yjaB NOLOHH_05425 0.44 +0.1 1,007,112 + yjaB NOLOHH_05425 0.45 +0.3 1,007,125 + yjaB NOLOHH_05425 0.48 +1.4 1,007,258 + yjaB NOLOHH_05425 0.78 +0.8 1,007,258 + yjaB NOLOHH_05425 0.78 -0.4 1,007,260 + yjaB NOLOHH_05425 0.79 +0.1 1,007,260 + yjaB NOLOHH_05425 0.79 -0.1 1,007,339 + -1.1 1,007,339 + -1.7 1,007,339 + -1.3 1,007,339 + -0.5 1,007,340 - -1.6 1,007,340 - -0.1
Or see this region's nucleotide sequence