Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bdcA and yjgH are separated by 130 nucleotides yjgH and pyrB are separated by 418 nucleotides pyrB and pyrI are separated by 12 nucleotides pyrI and ridA are separated by 72 nucleotides ridA and NOLOHH_04140 are separated by 65 nucleotides
NOLOHH_04115: bdcA - SDR family oxidoreductase, at 743,947 to 744,660
bdcA
NOLOHH_04120: yjgH - RutC family protein YjgH, at 744,791 to 745,186
yjgH
NOLOHH_04125: pyrB - aspartate carbamoyltransferase, at 745,605 to 746,540
pyrB
NOLOHH_04130: pyrI - aspartate carbamoyltransferase regulatory subunit, at 746,553 to 747,014
pyrI
NOLOHH_04135: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 747,087 to 747,473
ridA
NOLOHH_04140: NOLOHH_04140 - transposase, at 747,539 to 747,994
_04140
Position (kb) 745
746
747 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 744.633 kb on + strand at 744.633 kb on + strand at 744.677 kb on - strand at 744.714 kb on - strand at 744.745 kb on + strand at 744.746 kb on - strand at 744.751 kb on + strand at 744.796 kb on - strand at 744.836 kb on + strand, within yjgH at 744.836 kb on + strand, within yjgH at 744.836 kb on + strand, within yjgH at 744.837 kb on - strand, within yjgH at 744.837 kb on - strand, within yjgH at 744.837 kb on - strand, within yjgH at 744.852 kb on - strand, within yjgH at 744.863 kb on - strand, within yjgH at 744.900 kb on + strand, within yjgH at 744.901 kb on - strand, within yjgH at 744.901 kb on - strand, within yjgH at 744.901 kb on - strand, within yjgH at 744.964 kb on + strand, within yjgH at 745.010 kb on - strand, within yjgH at 745.119 kb on + strand, within yjgH at 745.120 kb on - strand, within yjgH at 745.126 kb on + strand, within yjgH at 745.126 kb on + strand, within yjgH at 745.127 kb on - strand, within yjgH at 745.127 kb on - strand, within yjgH at 745.127 kb on - strand, within yjgH at 745.165 kb on - strand at 745.184 kb on + strand at 745.185 kb on - strand at 745.191 kb on + strand at 745.191 kb on + strand at 745.191 kb on + strand at 745.191 kb on + strand at 745.192 kb on - strand at 745.223 kb on + strand at 745.225 kb on + strand at 745.226 kb on - strand at 745.226 kb on - strand at 745.253 kb on - strand at 745.302 kb on + strand at 745.303 kb on - strand at 745.466 kb on + strand at 745.466 kb on + strand at 745.466 kb on + strand at 745.466 kb on + strand at 745.466 kb on + strand at 745.467 kb on - strand at 745.467 kb on - strand at 745.467 kb on - strand at 745.467 kb on - strand at 745.618 kb on + strand at 745.632 kb on - strand at 745.632 kb on - strand at 745.632 kb on - strand at 745.793 kb on + strand, within pyrB at 745.793 kb on + strand, within pyrB at 745.793 kb on + strand, within pyrB at 745.793 kb on + strand, within pyrB at 745.793 kb on + strand, within pyrB at 745.793 kb on + strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.794 kb on - strand, within pyrB at 745.841 kb on + strand, within pyrB at 745.841 kb on + strand, within pyrB at 745.841 kb on + strand, within pyrB at 745.842 kb on - strand, within pyrB at 745.899 kb on + strand, within pyrB at 745.899 kb on + strand, within pyrB at 745.899 kb on + strand, within pyrB at 745.899 kb on + strand, within pyrB at 745.899 kb on + strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.900 kb on - strand, within pyrB at 745.973 kb on - strand, within pyrB at 745.973 kb on - strand, within pyrB at 746.032 kb on + strand, within pyrB at 746.032 kb on + strand, within pyrB at 746.032 kb on + strand, within pyrB at 746.033 kb on - strand, within pyrB at 746.033 kb on - strand, within pyrB at 746.160 kb on + strand, within pyrB at 746.161 kb on - strand, within pyrB at 746.196 kb on + strand, within pyrB at 746.196 kb on + strand, within pyrB at 746.196 kb on + strand, within pyrB at 746.208 kb on - strand, within pyrB at 746.272 kb on + strand, within pyrB at 746.272 kb on + strand, within pyrB at 746.283 kb on + strand, within pyrB at 746.283 kb on + strand, within pyrB at 746.284 kb on - strand, within pyrB at 746.284 kb on - strand, within pyrB at 746.284 kb on - strand, within pyrB at 746.284 kb on - strand, within pyrB at 746.441 kb on + strand, within pyrB at 746.441 kb on + strand, within pyrB at 746.548 kb on + strand at 746.548 kb on + strand at 746.548 kb on + strand at 746.585 kb on + strand at 746.586 kb on - strand at 746.586 kb on - strand at 746.589 kb on - strand at 746.602 kb on + strand, within pyrI at 746.603 kb on - strand, within pyrI at 746.633 kb on + strand, within pyrI at 746.633 kb on + strand, within pyrI at 746.633 kb on + strand, within pyrI at 746.633 kb on + strand, within pyrI at 746.634 kb on - strand, within pyrI at 746.634 kb on - strand, within pyrI at 746.781 kb on + strand, within pyrI at 746.782 kb on - strand, within pyrI at 746.817 kb on + strand, within pyrI at 746.818 kb on - strand, within pyrI at 746.818 kb on - strand, within pyrI at 746.831 kb on + strand, within pyrI at 746.894 kb on + strand, within pyrI at 746.895 kb on - strand, within pyrI at 746.895 kb on - strand, within pyrI at 746.970 kb on + strand at 746.971 kb on - strand at 746.993 kb on + strand at 746.993 kb on + strand at 747.013 kb on - strand at 747.013 kb on - strand at 747.028 kb on + strand at 747.028 kb on + strand at 747.028 kb on + strand at 747.028 kb on + strand at 747.029 kb on - strand at 747.044 kb on + strand at 747.045 kb on - strand at 747.045 kb on - strand at 747.071 kb on + strand at 747.074 kb on + strand at 747.074 kb on + strand at 747.074 kb on + strand at 747.075 kb on - strand at 747.135 kb on + strand, within ridA at 747.136 kb on - strand, within ridA at 747.136 kb on - strand, within ridA at 747.139 kb on + strand, within ridA at 747.139 kb on + strand, within ridA at 747.139 kb on + strand, within ridA at 747.161 kb on + strand, within ridA at 747.162 kb on - strand, within ridA at 747.256 kb on + strand, within ridA at 747.257 kb on - strand, within ridA at 747.257 kb on - strand, within ridA at 747.357 kb on + strand, within ridA at 747.357 kb on + strand, within ridA at 747.357 kb on + strand, within ridA at 747.358 kb on - strand, within ridA at 747.358 kb on - strand, within ridA at 747.472 kb on - strand at 747.478 kb on + strand at 747.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 744,633 + +0.8 744,633 + +1.0 744,677 - -1.5 744,714 - -1.3 744,745 + +1.4 744,746 - +0.8 744,751 + +0.4 744,796 - +0.2 744,836 + yjgH NOLOHH_04120 0.11 +1.0 744,836 + yjgH NOLOHH_04120 0.11 -0.7 744,836 + yjgH NOLOHH_04120 0.11 +0.5 744,837 - yjgH NOLOHH_04120 0.12 -0.0 744,837 - yjgH NOLOHH_04120 0.12 +0.3 744,837 - yjgH NOLOHH_04120 0.12 -0.6 744,852 - yjgH NOLOHH_04120 0.15 -0.4 744,863 - yjgH NOLOHH_04120 0.18 -1.7 744,900 + yjgH NOLOHH_04120 0.28 +0.6 744,901 - yjgH NOLOHH_04120 0.28 +0.2 744,901 - yjgH NOLOHH_04120 0.28 +0.1 744,901 - yjgH NOLOHH_04120 0.28 -1.1 744,964 + yjgH NOLOHH_04120 0.44 -2.6 745,010 - yjgH NOLOHH_04120 0.55 -1.9 745,119 + yjgH NOLOHH_04120 0.83 +0.2 745,120 - yjgH NOLOHH_04120 0.83 +0.3 745,126 + yjgH NOLOHH_04120 0.85 +1.3 745,126 + yjgH NOLOHH_04120 0.85 +0.2 745,127 - yjgH NOLOHH_04120 0.85 +0.7 745,127 - yjgH NOLOHH_04120 0.85 +1.0 745,127 - yjgH NOLOHH_04120 0.85 -0.8 745,165 - +0.7 745,184 + -1.6 745,185 - +0.1 745,191 + -2.0 745,191 + -0.7 745,191 + -0.4 745,191 + -1.8 745,192 - -0.6 745,223 + -0.3 745,225 + +0.2 745,226 - -3.3 745,226 - -0.2 745,253 - -1.1 745,302 + -4.0 745,303 - -0.0 745,466 + -3.0 745,466 + -0.0 745,466 + +2.4 745,466 + -1.8 745,466 + -0.6 745,467 - -4.0 745,467 - -3.2 745,467 - -3.5 745,467 - -3.6 745,618 + -5.2 745,632 - -4.2 745,632 - -1.4 745,632 - -4.8 745,793 + pyrB NOLOHH_04125 0.20 -3.8 745,793 + pyrB NOLOHH_04125 0.20 -2.5 745,793 + pyrB NOLOHH_04125 0.20 -4.5 745,793 + pyrB NOLOHH_04125 0.20 -3.5 745,793 + pyrB NOLOHH_04125 0.20 -1.6 745,793 + pyrB NOLOHH_04125 0.20 -2.9 745,794 - pyrB NOLOHH_04125 0.20 -4.5 745,794 - pyrB NOLOHH_04125 0.20 -4.5 745,794 - pyrB NOLOHH_04125 0.20 -5.2 745,794 - pyrB NOLOHH_04125 0.20 -5.3 745,794 - pyrB NOLOHH_04125 0.20 -1.4 745,794 - pyrB NOLOHH_04125 0.20 -4.4 745,794 - pyrB NOLOHH_04125 0.20 -4.5 745,794 - pyrB NOLOHH_04125 0.20 -3.1 745,841 + pyrB NOLOHH_04125 0.25 -1.6 745,841 + pyrB NOLOHH_04125 0.25 -5.2 745,841 + pyrB NOLOHH_04125 0.25 -5.0 745,842 - pyrB NOLOHH_04125 0.25 -4.8 745,899 + pyrB NOLOHH_04125 0.31 -5.5 745,899 + pyrB NOLOHH_04125 0.31 -4.3 745,899 + pyrB NOLOHH_04125 0.31 -3.1 745,899 + pyrB NOLOHH_04125 0.31 -0.7 745,899 + pyrB NOLOHH_04125 0.31 -3.6 745,900 - pyrB NOLOHH_04125 0.32 -2.5 745,900 - pyrB NOLOHH_04125 0.32 -5.3 745,900 - pyrB NOLOHH_04125 0.32 -3.8 745,900 - pyrB NOLOHH_04125 0.32 -6.1 745,900 - pyrB NOLOHH_04125 0.32 -3.8 745,900 - pyrB NOLOHH_04125 0.32 -2.4 745,900 - pyrB NOLOHH_04125 0.32 -2.9 745,900 - pyrB NOLOHH_04125 0.32 -3.3 745,973 - pyrB NOLOHH_04125 0.39 -4.3 745,973 - pyrB NOLOHH_04125 0.39 -4.9 746,032 + pyrB NOLOHH_04125 0.46 -1.2 746,032 + pyrB NOLOHH_04125 0.46 -5.4 746,032 + pyrB NOLOHH_04125 0.46 -4.8 746,033 - pyrB NOLOHH_04125 0.46 -4.2 746,033 - pyrB NOLOHH_04125 0.46 -3.8 746,160 + pyrB NOLOHH_04125 0.59 -3.6 746,161 - pyrB NOLOHH_04125 0.59 -3.1 746,196 + pyrB NOLOHH_04125 0.63 -2.3 746,196 + pyrB NOLOHH_04125 0.63 -2.4 746,196 + pyrB NOLOHH_04125 0.63 -5.2 746,208 - pyrB NOLOHH_04125 0.64 -2.6 746,272 + pyrB NOLOHH_04125 0.71 -4.4 746,272 + pyrB NOLOHH_04125 0.71 -3.7 746,283 + pyrB NOLOHH_04125 0.72 -2.2 746,283 + pyrB NOLOHH_04125 0.72 -2.3 746,284 - pyrB NOLOHH_04125 0.73 -5.0 746,284 - pyrB NOLOHH_04125 0.73 -3.3 746,284 - pyrB NOLOHH_04125 0.73 -4.4 746,284 - pyrB NOLOHH_04125 0.73 -3.3 746,441 + pyrB NOLOHH_04125 0.89 -3.3 746,441 + pyrB NOLOHH_04125 0.89 -3.4 746,548 + +0.7 746,548 + -0.6 746,548 + +0.5 746,585 + +1.1 746,586 - +0.1 746,586 - +0.4 746,589 - +0.4 746,602 + pyrI NOLOHH_04130 0.11 +0.7 746,603 - pyrI NOLOHH_04130 0.11 +0.7 746,633 + pyrI NOLOHH_04130 0.17 +2.3 746,633 + pyrI NOLOHH_04130 0.17 +2.1 746,633 + pyrI NOLOHH_04130 0.17 -0.5 746,633 + pyrI NOLOHH_04130 0.17 +0.1 746,634 - pyrI NOLOHH_04130 0.18 +0.9 746,634 - pyrI NOLOHH_04130 0.18 -0.0 746,781 + pyrI NOLOHH_04130 0.49 -1.3 746,782 - pyrI NOLOHH_04130 0.50 -0.0 746,817 + pyrI NOLOHH_04130 0.57 -1.6 746,818 - pyrI NOLOHH_04130 0.57 +0.0 746,818 - pyrI NOLOHH_04130 0.57 -2.1 746,831 + pyrI NOLOHH_04130 0.60 +0.5 746,894 + pyrI NOLOHH_04130 0.74 -0.1 746,895 - pyrI NOLOHH_04130 0.74 -0.1 746,895 - pyrI NOLOHH_04130 0.74 +0.8 746,970 + +0.7 746,971 - +0.4 746,993 + -0.7 746,993 + +0.9 747,013 - -0.3 747,013 - +0.2 747,028 + +0.5 747,028 + -0.1 747,028 + -1.2 747,028 + +0.2 747,029 - +0.1 747,044 + -0.2 747,045 - -0.2 747,045 - +0.4 747,071 + -0.6 747,074 + +0.8 747,074 + -0.3 747,074 + +0.1 747,075 - +0.4 747,135 + ridA NOLOHH_04135 0.12 +0.9 747,136 - ridA NOLOHH_04135 0.13 -0.4 747,136 - ridA NOLOHH_04135 0.13 -0.1 747,139 + ridA NOLOHH_04135 0.13 -1.3 747,139 + ridA NOLOHH_04135 0.13 -0.4 747,139 + ridA NOLOHH_04135 0.13 -1.7 747,161 + ridA NOLOHH_04135 0.19 -0.6 747,162 - ridA NOLOHH_04135 0.19 +0.6 747,256 + ridA NOLOHH_04135 0.44 -0.3 747,257 - ridA NOLOHH_04135 0.44 +0.1 747,257 - ridA NOLOHH_04135 0.44 +0.2 747,357 + ridA NOLOHH_04135 0.70 -0.4 747,357 + ridA NOLOHH_04135 0.70 +0.6 747,357 + ridA NOLOHH_04135 0.70 -0.8 747,358 - ridA NOLOHH_04135 0.70 -0.2 747,358 - ridA NOLOHH_04135 0.70 -0.2 747,472 - +0.8 747,478 + -0.0 747,479 - +0.7
Or see this region's nucleotide sequence