Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides thrA and NOLOHH_03445 are separated by 80 nucleotides NOLOHH_03445 and lasT are separated by 213 nucleotides
NOLOHH_03430: thrC - threonine synthase, at 604,616 to 605,902
thrC
NOLOHH_03435: thrB - homoserine kinase, at 605,903 to 606,835
thrB
NOLOHH_03440: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 606,837 to 609,299
thrA
NOLOHH_03445: NOLOHH_03445 - thr operon leader peptide, at 609,380 to 609,445
_03445
NOLOHH_03450: lasT - tRNA/rRNA methyltransferase, at 609,659 to 610,345
lasT
Position (kb) 606
607
608
609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 605.847 kb on - strand at 605.893 kb on - strand at 605.904 kb on + strand at 605.905 kb on - strand at 605.905 kb on - strand at 605.916 kb on - strand at 605.947 kb on + strand at 605.947 kb on + strand at 605.947 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.977 kb on - strand at 606.029 kb on + strand, within thrB at 606.029 kb on + strand, within thrB at 606.029 kb on + strand, within thrB at 606.030 kb on - strand, within thrB at 606.030 kb on - strand, within thrB at 606.263 kb on + strand, within thrB at 606.295 kb on + strand, within thrB at 606.549 kb on - strand, within thrB at 606.568 kb on + strand, within thrB at 606.640 kb on - strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.799 kb on + strand at 606.829 kb on + strand at 606.846 kb on + strand at 606.863 kb on + strand at 606.867 kb on + strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.917 kb on - strand at 606.917 kb on - strand at 606.958 kb on + strand at 606.958 kb on + strand at 606.959 kb on - strand at 607.055 kb on + strand at 607.066 kb on + strand at 607.066 kb on + strand at 607.067 kb on - strand at 607.067 kb on - strand at 607.145 kb on + strand, within thrA at 607.146 kb on - strand, within thrA at 607.271 kb on + strand, within thrA at 607.359 kb on + strand, within thrA at 607.359 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.375 kb on + strand, within thrA at 607.375 kb on + strand, within thrA at 607.375 kb on + strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.487 kb on - strand, within thrA at 607.487 kb on - strand, within thrA at 607.534 kb on + strand, within thrA at 607.534 kb on + strand, within thrA at 607.535 kb on - strand, within thrA at 607.535 kb on - strand, within thrA at 607.537 kb on + strand, within thrA at 607.538 kb on - strand, within thrA at 607.538 kb on - strand, within thrA at 607.538 kb on - strand, within thrA at 607.609 kb on + strand, within thrA at 607.610 kb on - strand, within thrA at 607.670 kb on - strand, within thrA at 607.704 kb on - strand, within thrA at 607.753 kb on - strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.807 kb on - strand, within thrA at 607.807 kb on - strand, within thrA at 607.815 kb on - strand, within thrA at 607.815 kb on - strand, within thrA at 608.084 kb on + strand, within thrA at 608.084 kb on + strand, within thrA at 608.085 kb on - strand, within thrA at 608.094 kb on + strand, within thrA at 608.094 kb on + strand, within thrA at 608.094 kb on + strand, within thrA at 608.094 kb on + strand, within thrA at 608.142 kb on + strand, within thrA at 608.143 kb on - strand, within thrA at 608.229 kb on - strand, within thrA at 608.233 kb on + strand, within thrA at 608.233 kb on + strand, within thrA at 608.233 kb on + strand, within thrA at 608.360 kb on + strand, within thrA at 608.361 kb on - strand, within thrA at 608.361 kb on - strand, within thrA at 608.524 kb on + strand, within thrA at 608.524 kb on + strand, within thrA at 608.525 kb on - strand, within thrA at 608.525 kb on - strand, within thrA at 608.525 kb on - strand, within thrA at 608.601 kb on - strand, within thrA at 608.602 kb on + strand, within thrA at 608.602 kb on + strand, within thrA at 608.603 kb on - strand, within thrA at 608.603 kb on - strand, within thrA at 608.603 kb on - strand, within thrA at 608.697 kb on + strand, within thrA at 608.810 kb on + strand, within thrA at 608.821 kb on + strand, within thrA at 608.821 kb on + strand, within thrA at 608.821 kb on + strand, within thrA at 608.822 kb on - strand, within thrA at 608.822 kb on - strand, within thrA at 608.886 kb on + strand, within thrA at 608.975 kb on + strand, within thrA at 608.975 kb on + strand, within thrA at 608.975 kb on + strand, within thrA at 608.975 kb on + strand, within thrA at 608.976 kb on - strand, within thrA at 608.976 kb on - strand, within thrA at 608.976 kb on - strand, within thrA at 608.976 kb on - strand, within thrA at 608.976 kb on - strand, within thrA at 608.976 kb on - strand, within thrA at 608.997 kb on + strand, within thrA at 608.997 kb on + strand, within thrA at 608.997 kb on + strand, within thrA at 609.276 kb on - strand at 609.307 kb on - strand at 609.307 kb on - strand at 609.375 kb on - strand at 609.422 kb on - strand, within NOLOHH_03445 at 609.433 kb on + strand, within NOLOHH_03445 at 609.457 kb on - strand at 609.470 kb on + strand at 609.485 kb on + strand at 609.486 kb on - strand at 609.492 kb on - strand at 609.552 kb on - strand at 609.605 kb on + strand at 609.605 kb on + strand at 609.605 kb on + strand at 609.605 kb on + strand at 609.605 kb on + strand at 609.605 kb on + strand at 609.606 kb on - strand at 609.606 kb on - strand at 609.606 kb on - strand at 609.606 kb on - strand at 609.642 kb on - strand at 609.645 kb on + strand at 609.648 kb on + strand at 609.660 kb on + strand at 609.670 kb on + strand at 609.682 kb on + strand at 609.764 kb on + strand, within lasT at 609.765 kb on - strand, within lasT at 609.765 kb on - strand, within lasT at 609.765 kb on - strand, within lasT at 609.765 kb on - strand, within lasT at 609.873 kb on - strand, within lasT at 609.894 kb on + strand, within lasT at 609.894 kb on + strand, within lasT at 609.894 kb on + strand, within lasT at 609.894 kb on + strand, within lasT at 609.895 kb on - strand, within lasT at 609.895 kb on - strand, within lasT at 609.931 kb on - strand, within lasT at 609.955 kb on + strand, within lasT at 609.975 kb on - strand, within lasT at 609.975 kb on - strand, within lasT at 610.052 kb on + strand, within lasT at 610.070 kb on + strand, within lasT at 610.086 kb on + strand, within lasT at 610.086 kb on + strand, within lasT at 610.086 kb on + strand, within lasT at 610.086 kb on + strand, within lasT at 610.087 kb on - strand, within lasT at 610.087 kb on - strand, within lasT at 610.087 kb on - strand, within lasT at 610.092 kb on + strand, within lasT at 610.092 kb on + strand, within lasT at 610.184 kb on - strand, within lasT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 605,847 - -1.2 605,893 - -0.6 605,904 + +0.5 605,905 - +0.4 605,905 - -0.4 605,916 - +0.1 605,947 + +1.2 605,947 + +0.2 605,947 + -0.6 605,976 + -0.0 605,976 + +0.7 605,976 + -2.0 605,976 + +0.1 605,976 + -0.0 605,976 + -0.7 605,977 - +0.8 606,029 + thrB NOLOHH_03435 0.14 -1.2 606,029 + thrB NOLOHH_03435 0.14 -0.5 606,029 + thrB NOLOHH_03435 0.14 +0.1 606,030 - thrB NOLOHH_03435 0.14 +0.5 606,030 - thrB NOLOHH_03435 0.14 +0.5 606,263 + thrB NOLOHH_03435 0.39 -0.3 606,295 + thrB NOLOHH_03435 0.42 +0.3 606,549 - thrB NOLOHH_03435 0.69 +0.0 606,568 + thrB NOLOHH_03435 0.71 -0.5 606,640 - thrB NOLOHH_03435 0.79 +0.1 606,731 + thrB NOLOHH_03435 0.89 +0.3 606,731 + thrB NOLOHH_03435 0.89 -1.5 606,731 + thrB NOLOHH_03435 0.89 -1.1 606,731 + thrB NOLOHH_03435 0.89 -0.4 606,732 - thrB NOLOHH_03435 0.89 -0.0 606,732 - thrB NOLOHH_03435 0.89 -0.4 606,732 - thrB NOLOHH_03435 0.89 +0.5 606,732 - thrB NOLOHH_03435 0.89 -0.3 606,732 - thrB NOLOHH_03435 0.89 -1.4 606,732 - thrB NOLOHH_03435 0.89 +0.2 606,732 - thrB NOLOHH_03435 0.89 +0.7 606,732 - thrB NOLOHH_03435 0.89 -0.0 606,732 - thrB NOLOHH_03435 0.89 +0.5 606,799 + -0.9 606,829 + +1.5 606,846 + +0.9 606,863 + +0.1 606,867 + +0.1 606,868 - +0.6 606,868 - +0.4 606,868 - -2.8 606,868 - +0.4 606,917 - -0.4 606,917 - +0.9 606,958 + +0.3 606,958 + -0.4 606,959 - -0.3 607,055 + -0.6 607,066 + -1.3 607,066 + +2.2 607,067 - -0.6 607,067 - +0.7 607,145 + thrA NOLOHH_03440 0.13 -1.8 607,146 - thrA NOLOHH_03440 0.13 +0.4 607,271 + thrA NOLOHH_03440 0.18 +1.3 607,359 + thrA NOLOHH_03440 0.21 -0.1 607,359 + thrA NOLOHH_03440 0.21 -0.3 607,373 + thrA NOLOHH_03440 0.22 +0.4 607,373 + thrA NOLOHH_03440 0.22 +0.1 607,373 + thrA NOLOHH_03440 0.22 +0.3 607,373 + thrA NOLOHH_03440 0.22 -0.2 607,374 - thrA NOLOHH_03440 0.22 +0.0 607,374 - thrA NOLOHH_03440 0.22 +0.4 607,374 - thrA NOLOHH_03440 0.22 +0.4 607,374 - thrA NOLOHH_03440 0.22 +1.6 607,374 - thrA NOLOHH_03440 0.22 +0.8 607,374 - thrA NOLOHH_03440 0.22 -0.2 607,375 + thrA NOLOHH_03440 0.22 +1.0 607,375 + thrA NOLOHH_03440 0.22 +0.3 607,375 + thrA NOLOHH_03440 0.22 -0.5 607,376 - thrA NOLOHH_03440 0.22 -1.0 607,376 - thrA NOLOHH_03440 0.22 -0.4 607,376 - thrA NOLOHH_03440 0.22 -0.3 607,376 - thrA NOLOHH_03440 0.22 +0.3 607,376 - thrA NOLOHH_03440 0.22 -0.4 607,376 - thrA NOLOHH_03440 0.22 +0.2 607,487 - thrA NOLOHH_03440 0.26 -0.1 607,487 - thrA NOLOHH_03440 0.26 +0.2 607,534 + thrA NOLOHH_03440 0.28 -0.2 607,534 + thrA NOLOHH_03440 0.28 +1.0 607,535 - thrA NOLOHH_03440 0.28 +0.7 607,535 - thrA NOLOHH_03440 0.28 +0.5 607,537 + thrA NOLOHH_03440 0.28 +0.6 607,538 - thrA NOLOHH_03440 0.28 +1.2 607,538 - thrA NOLOHH_03440 0.28 +0.4 607,538 - thrA NOLOHH_03440 0.28 +0.2 607,609 + thrA NOLOHH_03440 0.31 -1.4 607,610 - thrA NOLOHH_03440 0.31 +1.9 607,670 - thrA NOLOHH_03440 0.34 +0.2 607,704 - thrA NOLOHH_03440 0.35 -0.2 607,753 - thrA NOLOHH_03440 0.37 +0.4 607,806 + thrA NOLOHH_03440 0.39 +0.4 607,806 + thrA NOLOHH_03440 0.39 +1.3 607,806 + thrA NOLOHH_03440 0.39 +0.1 607,806 + thrA NOLOHH_03440 0.39 +0.2 607,807 - thrA NOLOHH_03440 0.39 +0.4 607,807 - thrA NOLOHH_03440 0.39 -0.5 607,815 - thrA NOLOHH_03440 0.40 -0.6 607,815 - thrA NOLOHH_03440 0.40 -0.1 608,084 + thrA NOLOHH_03440 0.51 +0.3 608,084 + thrA NOLOHH_03440 0.51 +0.5 608,085 - thrA NOLOHH_03440 0.51 -0.3 608,094 + thrA NOLOHH_03440 0.51 -0.1 608,094 + thrA NOLOHH_03440 0.51 +0.1 608,094 + thrA NOLOHH_03440 0.51 -2.5 608,094 + thrA NOLOHH_03440 0.51 +0.3 608,142 + thrA NOLOHH_03440 0.53 -0.2 608,143 - thrA NOLOHH_03440 0.53 +0.4 608,229 - thrA NOLOHH_03440 0.57 -0.2 608,233 + thrA NOLOHH_03440 0.57 +0.7 608,233 + thrA NOLOHH_03440 0.57 +0.2 608,233 + thrA NOLOHH_03440 0.57 -1.0 608,360 + thrA NOLOHH_03440 0.62 +0.2 608,361 - thrA NOLOHH_03440 0.62 -0.2 608,361 - thrA NOLOHH_03440 0.62 -0.5 608,524 + thrA NOLOHH_03440 0.68 +1.2 608,524 + thrA NOLOHH_03440 0.68 +0.2 608,525 - thrA NOLOHH_03440 0.69 +0.3 608,525 - thrA NOLOHH_03440 0.69 +1.0 608,525 - thrA NOLOHH_03440 0.69 +1.2 608,601 - thrA NOLOHH_03440 0.72 +0.3 608,602 + thrA NOLOHH_03440 0.72 -0.5 608,602 + thrA NOLOHH_03440 0.72 +0.5 608,603 - thrA NOLOHH_03440 0.72 +0.4 608,603 - thrA NOLOHH_03440 0.72 +0.3 608,603 - thrA NOLOHH_03440 0.72 +0.3 608,697 + thrA NOLOHH_03440 0.76 -0.7 608,810 + thrA NOLOHH_03440 0.80 +1.4 608,821 + thrA NOLOHH_03440 0.81 -0.3 608,821 + thrA NOLOHH_03440 0.81 -2.4 608,821 + thrA NOLOHH_03440 0.81 -0.3 608,822 - thrA NOLOHH_03440 0.81 +0.6 608,822 - thrA NOLOHH_03440 0.81 +0.3 608,886 + thrA NOLOHH_03440 0.83 -3.1 608,975 + thrA NOLOHH_03440 0.87 +0.4 608,975 + thrA NOLOHH_03440 0.87 -0.4 608,975 + thrA NOLOHH_03440 0.87 +0.4 608,975 + thrA NOLOHH_03440 0.87 +0.8 608,976 - thrA NOLOHH_03440 0.87 +0.3 608,976 - thrA NOLOHH_03440 0.87 -1.1 608,976 - thrA NOLOHH_03440 0.87 +0.4 608,976 - thrA NOLOHH_03440 0.87 +0.2 608,976 - thrA NOLOHH_03440 0.87 -0.9 608,976 - thrA NOLOHH_03440 0.87 +0.2 608,997 + thrA NOLOHH_03440 0.88 +1.0 608,997 + thrA NOLOHH_03440 0.88 -0.6 608,997 + thrA NOLOHH_03440 0.88 -2.5 609,276 - +0.8 609,307 - -0.3 609,307 - +0.4 609,375 - -0.4 609,422 - NOLOHH_03445 0.64 -0.0 609,433 + NOLOHH_03445 0.80 +1.6 609,457 - +0.0 609,470 + -2.0 609,485 + -0.1 609,486 - -0.4 609,492 - -0.2 609,552 - -0.3 609,605 + +1.4 609,605 + +0.6 609,605 + -0.3 609,605 + -0.4 609,605 + +0.6 609,605 + +1.3 609,606 - -0.4 609,606 - -2.3 609,606 - +0.2 609,606 - -1.2 609,642 - +1.0 609,645 + +0.7 609,648 + +0.0 609,660 + -0.8 609,670 + -0.7 609,682 + -1.0 609,764 + lasT NOLOHH_03450 0.15 +0.4 609,765 - lasT NOLOHH_03450 0.15 +0.8 609,765 - lasT NOLOHH_03450 0.15 +0.7 609,765 - lasT NOLOHH_03450 0.15 -0.3 609,765 - lasT NOLOHH_03450 0.15 +0.5 609,873 - lasT NOLOHH_03450 0.31 +0.7 609,894 + lasT NOLOHH_03450 0.34 -0.7 609,894 + lasT NOLOHH_03450 0.34 -1.4 609,894 + lasT NOLOHH_03450 0.34 +0.4 609,894 + lasT NOLOHH_03450 0.34 +1.4 609,895 - lasT NOLOHH_03450 0.34 -1.7 609,895 - lasT NOLOHH_03450 0.34 +0.2 609,931 - lasT NOLOHH_03450 0.40 +1.1 609,955 + lasT NOLOHH_03450 0.43 -0.7 609,975 - lasT NOLOHH_03450 0.46 +0.1 609,975 - lasT NOLOHH_03450 0.46 -0.7 610,052 + lasT NOLOHH_03450 0.57 +0.5 610,070 + lasT NOLOHH_03450 0.60 -0.1 610,086 + lasT NOLOHH_03450 0.62 -1.2 610,086 + lasT NOLOHH_03450 0.62 +0.3 610,086 + lasT NOLOHH_03450 0.62 -1.8 610,086 + lasT NOLOHH_03450 0.62 +1.7 610,087 - lasT NOLOHH_03450 0.62 +0.2 610,087 - lasT NOLOHH_03450 0.62 -0.0 610,087 - lasT NOLOHH_03450 0.62 +0.4 610,092 + lasT NOLOHH_03450 0.63 -0.2 610,092 + lasT NOLOHH_03450 0.63 -2.6 610,184 - lasT NOLOHH_03450 0.76 -0.2
Or see this region's nucleotide sequence