Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides
NOLOHH_03430: thrC - threonine synthase, at 604,616 to 605,902
thrC
NOLOHH_03435: thrB - homoserine kinase, at 605,903 to 606,835
thrB
NOLOHH_03440: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 606,837 to 609,299
thrA
Position (kb) 605
606
607 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 604.920 kb on + strand, within thrC at 605.226 kb on + strand, within thrC at 605.227 kb on - strand, within thrC at 605.269 kb on + strand, within thrC at 605.389 kb on + strand, within thrC at 605.389 kb on + strand, within thrC at 605.390 kb on - strand, within thrC at 605.390 kb on - strand, within thrC at 605.390 kb on - strand, within thrC at 605.564 kb on - strand, within thrC at 605.585 kb on - strand, within thrC at 605.847 kb on - strand at 605.893 kb on - strand at 605.904 kb on + strand at 605.905 kb on - strand at 605.905 kb on - strand at 605.916 kb on - strand at 605.947 kb on + strand at 605.947 kb on + strand at 605.947 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.976 kb on + strand at 605.977 kb on - strand at 606.029 kb on + strand, within thrB at 606.029 kb on + strand, within thrB at 606.029 kb on + strand, within thrB at 606.030 kb on - strand, within thrB at 606.030 kb on - strand, within thrB at 606.263 kb on + strand, within thrB at 606.295 kb on + strand, within thrB at 606.549 kb on - strand, within thrB at 606.568 kb on + strand, within thrB at 606.640 kb on - strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.731 kb on + strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.732 kb on - strand, within thrB at 606.799 kb on + strand at 606.829 kb on + strand at 606.846 kb on + strand at 606.863 kb on + strand at 606.867 kb on + strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.868 kb on - strand at 606.917 kb on - strand at 606.917 kb on - strand at 606.958 kb on + strand at 606.958 kb on + strand at 606.959 kb on - strand at 607.055 kb on + strand at 607.066 kb on + strand at 607.066 kb on + strand at 607.067 kb on - strand at 607.067 kb on - strand at 607.145 kb on + strand, within thrA at 607.146 kb on - strand, within thrA at 607.271 kb on + strand, within thrA at 607.359 kb on + strand, within thrA at 607.359 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.373 kb on + strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.374 kb on - strand, within thrA at 607.375 kb on + strand, within thrA at 607.375 kb on + strand, within thrA at 607.375 kb on + strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.376 kb on - strand, within thrA at 607.487 kb on - strand, within thrA at 607.487 kb on - strand, within thrA at 607.534 kb on + strand, within thrA at 607.534 kb on + strand, within thrA at 607.535 kb on - strand, within thrA at 607.535 kb on - strand, within thrA at 607.537 kb on + strand, within thrA at 607.538 kb on - strand, within thrA at 607.538 kb on - strand, within thrA at 607.538 kb on - strand, within thrA at 607.609 kb on + strand, within thrA at 607.610 kb on - strand, within thrA at 607.670 kb on - strand, within thrA at 607.704 kb on - strand, within thrA at 607.753 kb on - strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.806 kb on + strand, within thrA at 607.807 kb on - strand, within thrA at 607.807 kb on - strand, within thrA at 607.815 kb on - strand, within thrA at 607.815 kb on - strand, within thrA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 604,920 + thrC NOLOHH_03430 0.24 -0.3 605,226 + thrC NOLOHH_03430 0.47 +0.9 605,227 - thrC NOLOHH_03430 0.47 +0.0 605,269 + thrC NOLOHH_03430 0.51 +0.2 605,389 + thrC NOLOHH_03430 0.60 +1.4 605,389 + thrC NOLOHH_03430 0.60 -1.2 605,390 - thrC NOLOHH_03430 0.60 +0.1 605,390 - thrC NOLOHH_03430 0.60 -0.6 605,390 - thrC NOLOHH_03430 0.60 +1.3 605,564 - thrC NOLOHH_03430 0.74 -0.2 605,585 - thrC NOLOHH_03430 0.75 +1.1 605,847 - -1.2 605,893 - -0.6 605,904 + +0.5 605,905 - +0.4 605,905 - -0.4 605,916 - +0.1 605,947 + +1.2 605,947 + +0.2 605,947 + -0.6 605,976 + -0.0 605,976 + +0.7 605,976 + -2.0 605,976 + +0.1 605,976 + -0.0 605,976 + -0.7 605,977 - +0.8 606,029 + thrB NOLOHH_03435 0.14 -1.2 606,029 + thrB NOLOHH_03435 0.14 -0.5 606,029 + thrB NOLOHH_03435 0.14 +0.1 606,030 - thrB NOLOHH_03435 0.14 +0.5 606,030 - thrB NOLOHH_03435 0.14 +0.5 606,263 + thrB NOLOHH_03435 0.39 -0.3 606,295 + thrB NOLOHH_03435 0.42 +0.3 606,549 - thrB NOLOHH_03435 0.69 +0.0 606,568 + thrB NOLOHH_03435 0.71 -0.5 606,640 - thrB NOLOHH_03435 0.79 +0.1 606,731 + thrB NOLOHH_03435 0.89 +0.3 606,731 + thrB NOLOHH_03435 0.89 -1.5 606,731 + thrB NOLOHH_03435 0.89 -1.1 606,731 + thrB NOLOHH_03435 0.89 -0.4 606,732 - thrB NOLOHH_03435 0.89 -0.0 606,732 - thrB NOLOHH_03435 0.89 -0.4 606,732 - thrB NOLOHH_03435 0.89 +0.5 606,732 - thrB NOLOHH_03435 0.89 -0.3 606,732 - thrB NOLOHH_03435 0.89 -1.4 606,732 - thrB NOLOHH_03435 0.89 +0.2 606,732 - thrB NOLOHH_03435 0.89 +0.7 606,732 - thrB NOLOHH_03435 0.89 -0.0 606,732 - thrB NOLOHH_03435 0.89 +0.5 606,799 + -0.9 606,829 + +1.5 606,846 + +0.9 606,863 + +0.1 606,867 + +0.1 606,868 - +0.6 606,868 - +0.4 606,868 - -2.8 606,868 - +0.4 606,917 - -0.4 606,917 - +0.9 606,958 + +0.3 606,958 + -0.4 606,959 - -0.3 607,055 + -0.6 607,066 + -1.3 607,066 + +2.2 607,067 - -0.6 607,067 - +0.7 607,145 + thrA NOLOHH_03440 0.13 -1.8 607,146 - thrA NOLOHH_03440 0.13 +0.4 607,271 + thrA NOLOHH_03440 0.18 +1.3 607,359 + thrA NOLOHH_03440 0.21 -0.1 607,359 + thrA NOLOHH_03440 0.21 -0.3 607,373 + thrA NOLOHH_03440 0.22 +0.4 607,373 + thrA NOLOHH_03440 0.22 +0.1 607,373 + thrA NOLOHH_03440 0.22 +0.3 607,373 + thrA NOLOHH_03440 0.22 -0.2 607,374 - thrA NOLOHH_03440 0.22 +0.0 607,374 - thrA NOLOHH_03440 0.22 +0.4 607,374 - thrA NOLOHH_03440 0.22 +0.4 607,374 - thrA NOLOHH_03440 0.22 +1.6 607,374 - thrA NOLOHH_03440 0.22 +0.8 607,374 - thrA NOLOHH_03440 0.22 -0.2 607,375 + thrA NOLOHH_03440 0.22 +1.0 607,375 + thrA NOLOHH_03440 0.22 +0.3 607,375 + thrA NOLOHH_03440 0.22 -0.5 607,376 - thrA NOLOHH_03440 0.22 -1.0 607,376 - thrA NOLOHH_03440 0.22 -0.4 607,376 - thrA NOLOHH_03440 0.22 -0.3 607,376 - thrA NOLOHH_03440 0.22 +0.3 607,376 - thrA NOLOHH_03440 0.22 -0.4 607,376 - thrA NOLOHH_03440 0.22 +0.2 607,487 - thrA NOLOHH_03440 0.26 -0.1 607,487 - thrA NOLOHH_03440 0.26 +0.2 607,534 + thrA NOLOHH_03440 0.28 -0.2 607,534 + thrA NOLOHH_03440 0.28 +1.0 607,535 - thrA NOLOHH_03440 0.28 +0.7 607,535 - thrA NOLOHH_03440 0.28 +0.5 607,537 + thrA NOLOHH_03440 0.28 +0.6 607,538 - thrA NOLOHH_03440 0.28 +1.2 607,538 - thrA NOLOHH_03440 0.28 +0.4 607,538 - thrA NOLOHH_03440 0.28 +0.2 607,609 + thrA NOLOHH_03440 0.31 -1.4 607,610 - thrA NOLOHH_03440 0.31 +1.9 607,670 - thrA NOLOHH_03440 0.34 +0.2 607,704 - thrA NOLOHH_03440 0.35 -0.2 607,753 - thrA NOLOHH_03440 0.37 +0.4 607,806 + thrA NOLOHH_03440 0.39 +0.4 607,806 + thrA NOLOHH_03440 0.39 +1.3 607,806 + thrA NOLOHH_03440 0.39 +0.1 607,806 + thrA NOLOHH_03440 0.39 +0.2 607,807 - thrA NOLOHH_03440 0.39 +0.4 607,807 - thrA NOLOHH_03440 0.39 -0.5 607,815 - thrA NOLOHH_03440 0.40 -0.6 607,815 - thrA NOLOHH_03440 0.40 -0.1
Or see this region's nucleotide sequence