Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and carA are separated by 17 nucleotides carA and dapB are separated by 455 nucleotides
NOLOHH_03270: carB - carbamoyl-phosphate synthase large subunit, at 573,687 to 576,908
carB
NOLOHH_03275: carA - carbamoyl phosphate synthase small subunit, at 576,926 to 578,074
carA
NOLOHH_03285: dapB - 4-hydroxy-tetrahydrodipicolinate reductase, at 578,530 to 579,351
dapB
Position (kb) 576
577
578
579 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 575.948 kb on + strand, within carB at 576.262 kb on + strand, within carB at 576.361 kb on + strand, within carB at 576.362 kb on - strand, within carB at 576.362 kb on - strand, within carB at 576.507 kb on + strand, within carB at 576.508 kb on - strand, within carB at 576.536 kb on + strand, within carB at 576.566 kb on + strand, within carB at 576.566 kb on + strand, within carB at 576.715 kb on + strand at 576.716 kb on - strand at 576.806 kb on + strand at 576.806 kb on + strand at 576.806 kb on + strand at 576.806 kb on + strand at 576.806 kb on + strand at 576.807 kb on - strand at 576.807 kb on - strand at 576.807 kb on - strand at 576.807 kb on - strand at 576.807 kb on - strand at 576.807 kb on - strand at 576.826 kb on + strand at 576.826 kb on + strand at 576.881 kb on + strand at 576.888 kb on + strand at 576.888 kb on + strand at 576.889 kb on - strand at 576.890 kb on + strand at 576.891 kb on - strand at 576.891 kb on - strand at 576.896 kb on + strand at 576.897 kb on - strand at 576.918 kb on - strand at 576.928 kb on - strand at 576.940 kb on + strand at 576.945 kb on + strand at 576.956 kb on + strand at 576.957 kb on - strand at 577.064 kb on + strand, within carA at 577.087 kb on + strand, within carA at 577.087 kb on + strand, within carA at 577.088 kb on - strand, within carA at 577.088 kb on - strand, within carA at 577.088 kb on - strand, within carA at 577.088 kb on - strand, within carA at 577.088 kb on - strand, within carA at 577.326 kb on + strand, within carA at 577.486 kb on - strand, within carA at 577.571 kb on - strand, within carA at 577.572 kb on + strand, within carA at 577.572 kb on + strand, within carA at 577.573 kb on - strand, within carA at 577.573 kb on - strand, within carA at 577.713 kb on - strand, within carA at 577.759 kb on + strand, within carA at 577.760 kb on - strand, within carA at 577.792 kb on + strand, within carA at 577.796 kb on - strand, within carA at 577.850 kb on + strand, within carA at 577.851 kb on - strand, within carA at 577.851 kb on - strand, within carA at 577.898 kb on - strand, within carA at 577.905 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.909 kb on + strand, within carA at 577.910 kb on - strand, within carA at 577.910 kb on - strand, within carA at 577.910 kb on - strand, within carA at 577.910 kb on - strand, within carA at 577.975 kb on + strand at 577.975 kb on + strand at 577.975 kb on + strand at 577.975 kb on + strand at 578.015 kb on + strand at 578.057 kb on + strand at 578.068 kb on + strand at 578.069 kb on - strand at 578.134 kb on - strand at 578.212 kb on + strand at 578.265 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 575,948 + carB NOLOHH_03270 0.70 -2.3 576,262 + carB NOLOHH_03270 0.80 -4.3 576,361 + carB NOLOHH_03270 0.83 -4.7 576,362 - carB NOLOHH_03270 0.83 -4.7 576,362 - carB NOLOHH_03270 0.83 -1.6 576,507 + carB NOLOHH_03270 0.88 -3.9 576,508 - carB NOLOHH_03270 0.88 -3.9 576,536 + carB NOLOHH_03270 0.88 -4.5 576,566 + carB NOLOHH_03270 0.89 -4.2 576,566 + carB NOLOHH_03270 0.89 -5.4 576,715 + -2.7 576,716 - -4.5 576,806 + -4.6 576,806 + -1.8 576,806 + -3.8 576,806 + -4.0 576,806 + -4.3 576,807 - -1.8 576,807 - -3.3 576,807 - -2.3 576,807 - -4.8 576,807 - -3.1 576,807 - -4.0 576,826 + -2.6 576,826 + -5.2 576,881 + -3.8 576,888 + -1.5 576,888 + -0.8 576,889 - -4.3 576,890 + -3.5 576,891 - -4.3 576,891 - -4.5 576,896 + -5.3 576,897 - -1.1 576,918 - -0.8 576,928 - -0.3 576,940 + -4.1 576,945 + -4.6 576,956 + -4.2 576,957 - -1.5 577,064 + carA NOLOHH_03275 0.12 -3.8 577,087 + carA NOLOHH_03275 0.14 -4.4 577,087 + carA NOLOHH_03275 0.14 -3.5 577,088 - carA NOLOHH_03275 0.14 -3.7 577,088 - carA NOLOHH_03275 0.14 -3.2 577,088 - carA NOLOHH_03275 0.14 -2.8 577,088 - carA NOLOHH_03275 0.14 -1.0 577,088 - carA NOLOHH_03275 0.14 -3.8 577,326 + carA NOLOHH_03275 0.35 -3.1 577,486 - carA NOLOHH_03275 0.49 -4.8 577,571 - carA NOLOHH_03275 0.56 -0.4 577,572 + carA NOLOHH_03275 0.56 +0.8 577,572 + carA NOLOHH_03275 0.56 -2.1 577,573 - carA NOLOHH_03275 0.56 -4.9 577,573 - carA NOLOHH_03275 0.56 -2.2 577,713 - carA NOLOHH_03275 0.68 -2.8 577,759 + carA NOLOHH_03275 0.72 -4.1 577,760 - carA NOLOHH_03275 0.73 -3.2 577,792 + carA NOLOHH_03275 0.75 -3.5 577,796 - carA NOLOHH_03275 0.76 -3.6 577,850 + carA NOLOHH_03275 0.80 -3.8 577,851 - carA NOLOHH_03275 0.81 -4.6 577,851 - carA NOLOHH_03275 0.81 -2.4 577,898 - carA NOLOHH_03275 0.85 -5.6 577,905 + carA NOLOHH_03275 0.85 -4.3 577,909 + carA NOLOHH_03275 0.86 -1.9 577,909 + carA NOLOHH_03275 0.86 -2.6 577,909 + carA NOLOHH_03275 0.86 -4.0 577,909 + carA NOLOHH_03275 0.86 -2.2 577,909 + carA NOLOHH_03275 0.86 -5.5 577,909 + carA NOLOHH_03275 0.86 -0.9 577,910 - carA NOLOHH_03275 0.86 -3.4 577,910 - carA NOLOHH_03275 0.86 -4.7 577,910 - carA NOLOHH_03275 0.86 -4.7 577,910 - carA NOLOHH_03275 0.86 -5.2 577,975 + -3.1 577,975 + -1.2 577,975 + -3.0 577,975 + -1.6 578,015 + -4.3 578,057 + -6.3 578,068 + -1.3 578,069 - -4.5 578,134 - +0.5 578,212 + -2.2 578,265 + -1.0
Or see this region's nucleotide sequence