Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoA and cyoB are separated by 21 nucleotides cyoB and cyoC overlap by 11 nucleotides cyoC and cyoD overlap by 1 nucleotides cyoD and cyoE are separated by 11 nucleotides
NOLOHH_01295: cyoA - cytochrome o ubiquinol oxidase subunit II, at 152,324 to 153,271
cyoA
NOLOHH_01300: cyoB - cytochrome o ubiquinol oxidase subunit I, at 153,293 to 155,284
cyoB
NOLOHH_01305: cyoC - cytochrome o ubiquinol oxidase subunit III, at 155,274 to 155,888
cyoC
NOLOHH_01310: cyoD - cytochrome o ubiquinol oxidase subunit III, at 155,888 to 156,217
cyoD
NOLOHH_01315: cyoE - heme o synthase, at 156,229 to 157,119
cyoE
Position (kb) 153
154
155
156 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 152.339 kb on + strand at 152.339 kb on + strand at 152.340 kb on - strand at 152.382 kb on + strand at 152.383 kb on - strand at 152.383 kb on - strand at 152.412 kb on + strand at 152.412 kb on + strand at 152.455 kb on - strand, within cyoA at 152.542 kb on + strand, within cyoA at 152.551 kb on + strand, within cyoA at 152.551 kb on + strand, within cyoA at 152.551 kb on + strand, within cyoA at 152.552 kb on - strand, within cyoA at 152.552 kb on - strand, within cyoA at 152.555 kb on + strand, within cyoA at 152.556 kb on - strand, within cyoA at 152.581 kb on + strand, within cyoA at 152.582 kb on - strand, within cyoA at 152.611 kb on + strand, within cyoA at 152.611 kb on + strand, within cyoA at 152.611 kb on + strand, within cyoA at 152.611 kb on + strand, within cyoA at 152.612 kb on - strand, within cyoA at 152.612 kb on - strand, within cyoA at 152.612 kb on - strand, within cyoA at 152.612 kb on - strand, within cyoA at 152.612 kb on - strand, within cyoA at 152.616 kb on + strand, within cyoA at 152.616 kb on + strand, within cyoA at 152.616 kb on + strand, within cyoA at 152.617 kb on - strand, within cyoA at 152.704 kb on + strand, within cyoA at 152.873 kb on + strand, within cyoA at 152.874 kb on - strand, within cyoA at 152.930 kb on + strand, within cyoA at 152.930 kb on + strand, within cyoA at 152.930 kb on + strand, within cyoA at 152.931 kb on - strand, within cyoA at 152.931 kb on - strand, within cyoA at 152.939 kb on - strand, within cyoA at 152.939 kb on - strand, within cyoA at 152.939 kb on - strand, within cyoA at 152.939 kb on - strand, within cyoA at 152.952 kb on - strand, within cyoA at 152.989 kb on + strand, within cyoA at 152.989 kb on + strand, within cyoA at 152.989 kb on + strand, within cyoA at 152.990 kb on - strand, within cyoA at 152.990 kb on - strand, within cyoA at 153.102 kb on - strand, within cyoA at 153.167 kb on - strand, within cyoA at 153.230 kb on - strand at 153.288 kb on + strand at 153.288 kb on + strand at 153.288 kb on + strand at 153.288 kb on + strand at 153.289 kb on - strand at 153.289 kb on - strand at 153.350 kb on - strand at 153.364 kb on + strand at 153.365 kb on - strand at 153.401 kb on + strand at 153.401 kb on + strand at 153.402 kb on - strand at 153.402 kb on - strand at 153.419 kb on + strand at 153.419 kb on + strand at 153.420 kb on - strand at 153.454 kb on + strand at 153.466 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.473 kb on + strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.474 kb on - strand at 153.475 kb on + strand at 153.475 kb on + strand at 153.476 kb on - strand at 153.619 kb on + strand, within cyoB at 153.620 kb on - strand, within cyoB at 153.723 kb on + strand, within cyoB at 153.723 kb on + strand, within cyoB at 153.724 kb on - strand, within cyoB at 153.724 kb on - strand, within cyoB at 153.724 kb on - strand, within cyoB at 153.809 kb on + strand, within cyoB at 153.828 kb on + strand, within cyoB at 153.829 kb on - strand, within cyoB at 153.833 kb on + strand, within cyoB at 153.833 kb on + strand, within cyoB at 153.833 kb on + strand, within cyoB at 153.833 kb on + strand, within cyoB at 153.834 kb on - strand, within cyoB at 153.888 kb on + strand, within cyoB at 153.888 kb on + strand, within cyoB at 153.888 kb on + strand, within cyoB at 153.888 kb on + strand, within cyoB at 153.888 kb on + strand, within cyoB at 153.889 kb on - strand, within cyoB at 153.892 kb on + strand, within cyoB at 153.972 kb on + strand, within cyoB at 153.972 kb on + strand, within cyoB at 154.116 kb on - strand, within cyoB at 154.116 kb on - strand, within cyoB at 154.116 kb on - strand, within cyoB at 154.154 kb on + strand, within cyoB at 154.154 kb on + strand, within cyoB at 154.155 kb on - strand, within cyoB at 154.229 kb on + strand, within cyoB at 154.232 kb on - strand, within cyoB at 154.258 kb on + strand, within cyoB at 154.258 kb on + strand, within cyoB at 154.259 kb on - strand, within cyoB at 154.323 kb on + strand, within cyoB at 154.324 kb on - strand, within cyoB at 154.355 kb on - strand, within cyoB at 154.403 kb on + strand, within cyoB at 154.403 kb on + strand, within cyoB at 154.403 kb on + strand, within cyoB at 154.403 kb on + strand, within cyoB at 154.403 kb on + strand, within cyoB at 154.404 kb on - strand, within cyoB at 154.404 kb on - strand, within cyoB at 154.404 kb on - strand, within cyoB at 154.525 kb on + strand, within cyoB at 154.562 kb on - strand, within cyoB at 154.605 kb on - strand, within cyoB at 154.652 kb on - strand, within cyoB at 154.706 kb on + strand, within cyoB at 154.800 kb on + strand, within cyoB at 154.841 kb on + strand, within cyoB at 154.842 kb on - strand, within cyoB at 154.940 kb on + strand, within cyoB at 154.940 kb on + strand, within cyoB at 154.940 kb on + strand, within cyoB at 154.941 kb on - strand, within cyoB at 154.943 kb on - strand, within cyoB at 154.943 kb on - strand, within cyoB at 155.012 kb on + strand, within cyoB at 155.031 kb on - strand, within cyoB at 155.031 kb on - strand, within cyoB at 155.045 kb on - strand, within cyoB at 155.119 kb on + strand at 155.119 kb on + strand at 155.119 kb on + strand at 155.120 kb on - strand at 155.120 kb on - strand at 155.198 kb on + strand at 155.201 kb on + strand at 155.201 kb on + strand at 155.201 kb on + strand at 155.202 kb on - strand at 155.202 kb on - strand at 155.202 kb on - strand at 155.202 kb on - strand at 155.254 kb on + strand at 155.255 kb on - strand at 155.285 kb on + strand at 155.285 kb on + strand at 155.408 kb on + strand, within cyoC at 155.409 kb on - strand, within cyoC at 155.409 kb on - strand, within cyoC at 155.409 kb on - strand, within cyoC at 155.412 kb on + strand, within cyoC at 155.413 kb on - strand, within cyoC at 155.520 kb on + strand, within cyoC at 155.545 kb on - strand, within cyoC at 155.567 kb on + strand, within cyoC at 155.567 kb on + strand, within cyoC at 155.567 kb on + strand, within cyoC at 155.625 kb on + strand, within cyoC at 155.625 kb on + strand, within cyoC at 155.626 kb on - strand, within cyoC at 155.735 kb on + strand, within cyoC at 155.758 kb on + strand, within cyoC at 155.758 kb on + strand, within cyoC at 155.869 kb on - strand at 155.869 kb on - strand at 155.886 kb on + strand at 155.886 kb on + strand at 155.886 kb on + strand at 155.886 kb on + strand at 155.887 kb on - strand at 155.931 kb on + strand, within cyoD at 155.939 kb on + strand, within cyoD at 156.054 kb on + strand, within cyoD at 156.094 kb on + strand, within cyoD at 156.145 kb on - strand, within cyoD at 156.145 kb on - strand, within cyoD at 156.166 kb on - strand, within cyoD at 156.166 kb on - strand, within cyoD at 156.181 kb on + strand, within cyoD at 156.182 kb on - strand, within cyoD at 156.197 kb on + strand at 156.197 kb on + strand at 156.198 kb on - strand at 156.198 kb on - strand at 156.198 kb on - strand at 156.215 kb on + strand at 156.216 kb on - strand at 156.228 kb on + strand at 156.237 kb on + strand at 156.237 kb on + strand at 156.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 152,339 + -1.4 152,339 + -0.7 152,340 - -0.9 152,382 + +0.3 152,383 - -0.0 152,383 - -0.5 152,412 + -0.8 152,412 + -1.6 152,455 - cyoA NOLOHH_01295 0.14 -2.3 152,542 + cyoA NOLOHH_01295 0.23 -0.3 152,551 + cyoA NOLOHH_01295 0.24 -0.7 152,551 + cyoA NOLOHH_01295 0.24 +0.1 152,551 + cyoA NOLOHH_01295 0.24 -2.7 152,552 - cyoA NOLOHH_01295 0.24 +0.7 152,552 - cyoA NOLOHH_01295 0.24 -1.3 152,555 + cyoA NOLOHH_01295 0.24 -0.2 152,556 - cyoA NOLOHH_01295 0.24 -2.7 152,581 + cyoA NOLOHH_01295 0.27 -0.8 152,582 - cyoA NOLOHH_01295 0.27 -0.7 152,611 + cyoA NOLOHH_01295 0.30 -1.3 152,611 + cyoA NOLOHH_01295 0.30 -0.9 152,611 + cyoA NOLOHH_01295 0.30 -0.8 152,611 + cyoA NOLOHH_01295 0.30 -0.7 152,612 - cyoA NOLOHH_01295 0.30 -1.1 152,612 - cyoA NOLOHH_01295 0.30 -2.4 152,612 - cyoA NOLOHH_01295 0.30 -1.4 152,612 - cyoA NOLOHH_01295 0.30 -0.4 152,612 - cyoA NOLOHH_01295 0.30 -0.9 152,616 + cyoA NOLOHH_01295 0.31 -0.9 152,616 + cyoA NOLOHH_01295 0.31 -2.9 152,616 + cyoA NOLOHH_01295 0.31 -0.1 152,617 - cyoA NOLOHH_01295 0.31 -0.3 152,704 + cyoA NOLOHH_01295 0.40 +0.1 152,873 + cyoA NOLOHH_01295 0.58 +0.4 152,874 - cyoA NOLOHH_01295 0.58 -0.9 152,930 + cyoA NOLOHH_01295 0.64 -2.2 152,930 + cyoA NOLOHH_01295 0.64 -0.4 152,930 + cyoA NOLOHH_01295 0.64 -1.2 152,931 - cyoA NOLOHH_01295 0.64 -1.4 152,931 - cyoA NOLOHH_01295 0.64 -1.7 152,939 - cyoA NOLOHH_01295 0.65 -0.1 152,939 - cyoA NOLOHH_01295 0.65 -3.0 152,939 - cyoA NOLOHH_01295 0.65 -2.8 152,939 - cyoA NOLOHH_01295 0.65 -2.2 152,952 - cyoA NOLOHH_01295 0.66 -0.0 152,989 + cyoA NOLOHH_01295 0.70 -1.5 152,989 + cyoA NOLOHH_01295 0.70 -1.6 152,989 + cyoA NOLOHH_01295 0.70 +0.2 152,990 - cyoA NOLOHH_01295 0.70 +0.4 152,990 - cyoA NOLOHH_01295 0.70 -0.7 153,102 - cyoA NOLOHH_01295 0.82 -2.8 153,167 - cyoA NOLOHH_01295 0.89 -1.3 153,230 - -0.6 153,288 + -1.2 153,288 + -1.5 153,288 + -0.7 153,288 + -1.0 153,289 - +0.0 153,289 - -0.3 153,350 - -0.8 153,364 + -0.7 153,365 - -1.8 153,401 + -0.7 153,401 + -0.5 153,402 - -0.9 153,402 - +0.1 153,419 + +1.8 153,419 + -1.5 153,420 - -2.0 153,454 + +1.2 153,466 + -1.0 153,473 + +0.0 153,473 + -1.6 153,473 + -1.1 153,473 + -1.0 153,473 + -0.5 153,473 + -0.6 153,473 + -0.2 153,473 + -3.2 153,473 + -0.7 153,473 + -0.2 153,473 + -0.1 153,474 - -4.3 153,474 - -1.5 153,474 - -1.9 153,474 - -0.6 153,474 - -2.0 153,474 - -1.1 153,474 - -0.7 153,474 - -0.7 153,474 - -0.3 153,474 - -0.5 153,475 + +0.0 153,475 + -1.2 153,476 - -3.0 153,619 + cyoB NOLOHH_01300 0.16 -1.6 153,620 - cyoB NOLOHH_01300 0.16 -0.7 153,723 + cyoB NOLOHH_01300 0.22 -2.0 153,723 + cyoB NOLOHH_01300 0.22 +0.5 153,724 - cyoB NOLOHH_01300 0.22 -1.6 153,724 - cyoB NOLOHH_01300 0.22 -1.2 153,724 - cyoB NOLOHH_01300 0.22 -0.7 153,809 + cyoB NOLOHH_01300 0.26 -1.1 153,828 + cyoB NOLOHH_01300 0.27 -1.4 153,829 - cyoB NOLOHH_01300 0.27 -1.9 153,833 + cyoB NOLOHH_01300 0.27 -2.0 153,833 + cyoB NOLOHH_01300 0.27 -1.1 153,833 + cyoB NOLOHH_01300 0.27 -1.0 153,833 + cyoB NOLOHH_01300 0.27 -0.5 153,834 - cyoB NOLOHH_01300 0.27 -1.0 153,888 + cyoB NOLOHH_01300 0.30 -1.5 153,888 + cyoB NOLOHH_01300 0.30 -4.3 153,888 + cyoB NOLOHH_01300 0.30 -0.9 153,888 + cyoB NOLOHH_01300 0.30 +0.2 153,888 + cyoB NOLOHH_01300 0.30 -1.1 153,889 - cyoB NOLOHH_01300 0.30 -0.5 153,892 + cyoB NOLOHH_01300 0.30 -0.5 153,972 + cyoB NOLOHH_01300 0.34 -1.2 153,972 + cyoB NOLOHH_01300 0.34 -2.5 154,116 - cyoB NOLOHH_01300 0.41 -3.2 154,116 - cyoB NOLOHH_01300 0.41 +0.4 154,116 - cyoB NOLOHH_01300 0.41 -0.3 154,154 + cyoB NOLOHH_01300 0.43 -2.6 154,154 + cyoB NOLOHH_01300 0.43 -0.5 154,155 - cyoB NOLOHH_01300 0.43 -3.7 154,229 + cyoB NOLOHH_01300 0.47 -2.0 154,232 - cyoB NOLOHH_01300 0.47 +0.9 154,258 + cyoB NOLOHH_01300 0.48 -1.7 154,258 + cyoB NOLOHH_01300 0.48 -1.3 154,259 - cyoB NOLOHH_01300 0.48 -1.0 154,323 + cyoB NOLOHH_01300 0.52 -0.5 154,324 - cyoB NOLOHH_01300 0.52 -0.2 154,355 - cyoB NOLOHH_01300 0.53 -0.9 154,403 + cyoB NOLOHH_01300 0.56 -1.3 154,403 + cyoB NOLOHH_01300 0.56 -0.5 154,403 + cyoB NOLOHH_01300 0.56 -1.1 154,403 + cyoB NOLOHH_01300 0.56 -3.8 154,403 + cyoB NOLOHH_01300 0.56 -2.1 154,404 - cyoB NOLOHH_01300 0.56 -0.7 154,404 - cyoB NOLOHH_01300 0.56 -0.9 154,404 - cyoB NOLOHH_01300 0.56 -2.2 154,525 + cyoB NOLOHH_01300 0.62 -1.4 154,562 - cyoB NOLOHH_01300 0.64 -3.1 154,605 - cyoB NOLOHH_01300 0.66 -0.5 154,652 - cyoB NOLOHH_01300 0.68 -0.7 154,706 + cyoB NOLOHH_01300 0.71 -1.0 154,800 + cyoB NOLOHH_01300 0.76 +0.3 154,841 + cyoB NOLOHH_01300 0.78 -0.7 154,842 - cyoB NOLOHH_01300 0.78 -0.8 154,940 + cyoB NOLOHH_01300 0.83 -0.9 154,940 + cyoB NOLOHH_01300 0.83 -1.4 154,940 + cyoB NOLOHH_01300 0.83 -2.3 154,941 - cyoB NOLOHH_01300 0.83 -1.3 154,943 - cyoB NOLOHH_01300 0.83 -1.4 154,943 - cyoB NOLOHH_01300 0.83 -1.3 155,012 + cyoB NOLOHH_01300 0.86 -0.7 155,031 - cyoB NOLOHH_01300 0.87 -1.0 155,031 - cyoB NOLOHH_01300 0.87 -4.7 155,045 - cyoB NOLOHH_01300 0.88 -0.7 155,119 + -0.4 155,119 + -0.7 155,119 + -2.9 155,120 - -1.4 155,120 - +0.1 155,198 + +0.0 155,201 + +0.5 155,201 + -0.2 155,201 + -0.3 155,202 - +1.9 155,202 - +1.3 155,202 - -0.7 155,202 - -0.1 155,254 + -1.2 155,255 - -1.5 155,285 + -0.9 155,285 + -1.9 155,408 + cyoC NOLOHH_01305 0.22 -0.8 155,409 - cyoC NOLOHH_01305 0.22 -1.7 155,409 - cyoC NOLOHH_01305 0.22 -1.4 155,409 - cyoC NOLOHH_01305 0.22 -0.7 155,412 + cyoC NOLOHH_01305 0.22 -0.1 155,413 - cyoC NOLOHH_01305 0.23 -0.5 155,520 + cyoC NOLOHH_01305 0.40 -1.5 155,545 - cyoC NOLOHH_01305 0.44 -2.2 155,567 + cyoC NOLOHH_01305 0.48 -0.4 155,567 + cyoC NOLOHH_01305 0.48 -1.4 155,567 + cyoC NOLOHH_01305 0.48 -3.4 155,625 + cyoC NOLOHH_01305 0.57 -1.5 155,625 + cyoC NOLOHH_01305 0.57 -0.4 155,626 - cyoC NOLOHH_01305 0.57 +0.3 155,735 + cyoC NOLOHH_01305 0.75 +0.1 155,758 + cyoC NOLOHH_01305 0.79 -3.0 155,758 + cyoC NOLOHH_01305 0.79 -2.9 155,869 - +0.6 155,869 - +0.3 155,886 + +0.0 155,886 + -0.9 155,886 + -1.3 155,886 + -1.4 155,887 - +0.8 155,931 + cyoD NOLOHH_01310 0.13 -2.4 155,939 + cyoD NOLOHH_01310 0.15 -1.3 156,054 + cyoD NOLOHH_01310 0.50 -0.8 156,094 + cyoD NOLOHH_01310 0.62 -0.8 156,145 - cyoD NOLOHH_01310 0.78 +0.1 156,145 - cyoD NOLOHH_01310 0.78 -2.6 156,166 - cyoD NOLOHH_01310 0.84 -1.9 156,166 - cyoD NOLOHH_01310 0.84 -0.7 156,181 + cyoD NOLOHH_01310 0.89 -1.7 156,182 - cyoD NOLOHH_01310 0.89 -1.0 156,197 + -1.9 156,197 + -1.0 156,198 - -0.7 156,198 - -0.6 156,198 - -1.8 156,215 + -1.1 156,216 - -1.3 156,228 + -1.3 156,237 + +0.1 156,237 + -1.5 156,255 - +0.5
Or see this region's nucleotide sequence