Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxH and purE are separated by 117 nucleotides purE and purK overlap by 4 nucleotides purK and arcC are separated by 136 nucleotides
NOLOHH_00820: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 49,171 to 49,893
lpxH
NOLOHH_00825: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 50,011 to 50,520
purE
NOLOHH_00830: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 50,517 to 51,584
purK
NOLOHH_00835: arcC - carbamate kinase, at 51,721 to 52,614
arcC
Position (kb) 50
51
52 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 50.003 kb on + strand at 50.003 kb on + strand at 50.026 kb on - strand at 50.209 kb on - strand, within purE at 50.323 kb on + strand, within purE at 50.333 kb on + strand, within purE at 50.334 kb on - strand, within purE at 50.334 kb on - strand, within purE at 50.442 kb on - strand, within purE at 50.552 kb on - strand at 50.558 kb on + strand at 50.559 kb on - strand at 50.812 kb on + strand, within purK at 50.812 kb on + strand, within purK at 50.812 kb on + strand, within purK at 50.813 kb on - strand, within purK at 50.854 kb on + strand, within purK at 50.873 kb on + strand, within purK at 50.966 kb on + strand, within purK at 50.966 kb on + strand, within purK at 50.966 kb on + strand, within purK at 50.966 kb on + strand, within purK at 50.967 kb on - strand, within purK at 50.967 kb on - strand, within purK at 50.985 kb on - strand, within purK at 51.060 kb on - strand, within purK at 51.077 kb on + strand, within purK at 51.171 kb on + strand, within purK at 51.172 kb on - strand, within purK at 51.348 kb on - strand, within purK at 51.348 kb on - strand, within purK at 51.543 kb on + strand at 51.572 kb on + strand at 51.573 kb on - strand at 51.573 kb on - strand at 51.583 kb on - strand at 51.617 kb on - strand at 51.655 kb on - strand at 51.655 kb on - strand at 51.664 kb on - strand at 51.733 kb on - strand at 51.798 kb on + strand at 51.799 kb on - strand at 51.815 kb on + strand, within arcC at 51.874 kb on + strand, within arcC at 51.934 kb on + strand, within arcC at 52.012 kb on - strand, within arcC at 52.012 kb on - strand, within arcC at 52.171 kb on - strand, within arcC at 52.246 kb on - strand, within arcC at 52.356 kb on + strand, within arcC at 52.357 kb on - strand, within arcC at 52.357 kb on - strand, within arcC at 52.357 kb on - strand, within arcC at 52.359 kb on - strand, within arcC at 52.411 kb on + strand, within arcC at 52.411 kb on + strand, within arcC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 50,003 + -3.1 50,003 + -3.9 50,026 - -1.9 50,209 - purE NOLOHH_00825 0.39 -5.5 50,323 + purE NOLOHH_00825 0.61 -4.0 50,333 + purE NOLOHH_00825 0.63 -5.3 50,334 - purE NOLOHH_00825 0.63 -4.7 50,334 - purE NOLOHH_00825 0.63 -3.8 50,442 - purE NOLOHH_00825 0.85 -4.7 50,552 - -4.8 50,558 + -4.0 50,559 - -4.4 50,812 + purK NOLOHH_00830 0.28 -5.8 50,812 + purK NOLOHH_00830 0.28 -4.6 50,812 + purK NOLOHH_00830 0.28 -4.6 50,813 - purK NOLOHH_00830 0.28 -3.9 50,854 + purK NOLOHH_00830 0.32 -3.6 50,873 + purK NOLOHH_00830 0.33 -1.1 50,966 + purK NOLOHH_00830 0.42 -1.0 50,966 + purK NOLOHH_00830 0.42 -5.3 50,966 + purK NOLOHH_00830 0.42 -4.9 50,966 + purK NOLOHH_00830 0.42 -3.6 50,967 - purK NOLOHH_00830 0.42 -4.8 50,967 - purK NOLOHH_00830 0.42 -4.6 50,985 - purK NOLOHH_00830 0.44 -1.6 51,060 - purK NOLOHH_00830 0.51 -4.0 51,077 + purK NOLOHH_00830 0.52 -5.5 51,171 + purK NOLOHH_00830 0.61 -0.5 51,172 - purK NOLOHH_00830 0.61 -5.2 51,348 - purK NOLOHH_00830 0.78 -4.2 51,348 - purK NOLOHH_00830 0.78 -3.1 51,543 + -6.3 51,572 + -0.3 51,573 - -3.1 51,573 - -3.5 51,583 - -4.5 51,617 - +1.4 51,655 - +1.0 51,655 - -0.1 51,664 - -0.6 51,733 - +0.6 51,798 + +1.7 51,799 - +1.4 51,815 + arcC NOLOHH_00835 0.11 +0.0 51,874 + arcC NOLOHH_00835 0.17 +0.2 51,934 + arcC NOLOHH_00835 0.24 +1.0 52,012 - arcC NOLOHH_00835 0.33 -2.5 52,012 - arcC NOLOHH_00835 0.33 -0.6 52,171 - arcC NOLOHH_00835 0.50 -2.1 52,246 - arcC NOLOHH_00835 0.59 +0.6 52,356 + arcC NOLOHH_00835 0.71 -4.6 52,357 - arcC NOLOHH_00835 0.71 -0.1 52,357 - arcC NOLOHH_00835 0.71 -2.2 52,357 - arcC NOLOHH_00835 0.71 -0.7 52,359 - arcC NOLOHH_00835 0.71 +0.1 52,411 + arcC NOLOHH_00835 0.77 -0.4 52,411 + arcC NOLOHH_00835 0.77 +0.9
Or see this region's nucleotide sequence