Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00825

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppiB and lpxH are separated by 2 nucleotideslpxH and purE are separated by 117 nucleotidespurE and purK overlap by 4 nucleotides NOLOHH_00815: ppiB - peptidylprolyl isomerase B, at 48,674 to 49,168 ppiB NOLOHH_00820: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 49,171 to 49,893 lpxH NOLOHH_00825: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 50,011 to 50,520 purE NOLOHH_00830: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 50,517 to 51,584 purK Position (kb) 50 51Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 50.003 kb on + strandat 50.003 kb on + strandat 50.026 kb on - strandat 50.209 kb on - strand, within purEat 50.323 kb on + strand, within purEat 50.333 kb on + strand, within purEat 50.334 kb on - strand, within purEat 50.334 kb on - strand, within purEat 50.442 kb on - strand, within purEat 50.552 kb on - strandat 50.558 kb on + strandat 50.559 kb on - strandat 50.812 kb on + strand, within purKat 50.812 kb on + strand, within purKat 50.812 kb on + strand, within purKat 50.813 kb on - strand, within purKat 50.854 kb on + strand, within purKat 50.873 kb on + strand, within purKat 50.966 kb on + strand, within purKat 50.966 kb on + strand, within purKat 50.966 kb on + strand, within purKat 50.966 kb on + strand, within purKat 50.967 kb on - strand, within purKat 50.967 kb on - strand, within purKat 50.985 kb on - strand, within purKat 51.060 kb on - strand, within purKat 51.077 kb on + strand, within purKat 51.171 kb on + strand, within purKat 51.172 kb on - strand, within purKat 51.348 kb on - strand, within purKat 51.348 kb on - strand, within purK

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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50,003 + -3.1
50,003 + -3.9
50,026 - -1.9
50,209 - purE NOLOHH_00825 0.39 -5.5
50,323 + purE NOLOHH_00825 0.61 -4.0
50,333 + purE NOLOHH_00825 0.63 -5.3
50,334 - purE NOLOHH_00825 0.63 -4.7
50,334 - purE NOLOHH_00825 0.63 -3.8
50,442 - purE NOLOHH_00825 0.85 -4.7
50,552 - -4.8
50,558 + -4.0
50,559 - -4.4
50,812 + purK NOLOHH_00830 0.28 -5.8
50,812 + purK NOLOHH_00830 0.28 -4.6
50,812 + purK NOLOHH_00830 0.28 -4.6
50,813 - purK NOLOHH_00830 0.28 -3.9
50,854 + purK NOLOHH_00830 0.32 -3.6
50,873 + purK NOLOHH_00830 0.33 -1.1
50,966 + purK NOLOHH_00830 0.42 -1.0
50,966 + purK NOLOHH_00830 0.42 -5.3
50,966 + purK NOLOHH_00830 0.42 -4.9
50,966 + purK NOLOHH_00830 0.42 -3.6
50,967 - purK NOLOHH_00830 0.42 -4.8
50,967 - purK NOLOHH_00830 0.42 -4.6
50,985 - purK NOLOHH_00830 0.44 -1.6
51,060 - purK NOLOHH_00830 0.51 -4.0
51,077 + purK NOLOHH_00830 0.52 -5.5
51,171 + purK NOLOHH_00830 0.61 -0.5
51,172 - purK NOLOHH_00830 0.61 -5.2
51,348 - purK NOLOHH_00830 0.78 -4.2
51,348 - purK NOLOHH_00830 0.78 -3.1

Or see this region's nucleotide sequence