Strain Fitness in Escherichia coli ECRC62 around BNILDI_14500

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegI and yegD are separated by 11 nucleotidesyegD and alkA are separated by 133 nucleotides BNILDI_14495: yegI - protein kinase YegI, at 2,923,568 to 2,925,508 yegI BNILDI_14500: yegD - molecular chaperone, at 2,925,520 to 2,926,872 yegD BNILDI_14505: alkA - DNA-3-methyladenine glycosylase 2, at 2,927,006 to 2,927,863 alkA Position (kb) 2925 2926 2927Strain fitness (log2 ratio) -2 -1 0 1 2at 2924.542 kb on + strand, within yegIat 2924.738 kb on + strand, within yegIat 2924.739 kb on - strand, within yegIat 2924.811 kb on - strand, within yegIat 2924.816 kb on - strand, within yegIat 2924.873 kb on + strand, within yegIat 2924.875 kb on + strand, within yegIat 2924.876 kb on - strand, within yegIat 2924.938 kb on - strand, within yegIat 2925.453 kb on + strandat 2925.875 kb on - strand, within yegDat 2926.272 kb on - strand, within yegDat 2926.586 kb on + strand, within yegDat 2926.586 kb on + strand, within yegDat 2926.586 kb on + strand, within yegDat 2926.586 kb on + strand, within yegDat 2926.586 kb on + strand, within yegDat 2926.587 kb on - strand, within yegDat 2926.653 kb on + strand, within yegDat 2926.673 kb on - strand, within yegDat 2926.801 kb on + strandat 2926.866 kb on + strandat 2926.867 kb on - strandat 2926.878 kb on - strandat 2926.975 kb on + strandat 2926.976 kb on - strandat 2927.009 kb on + strandat 2927.009 kb on + strandat 2927.034 kb on - strandat 2927.105 kb on + strand, within alkAat 2927.105 kb on + strand, within alkAat 2927.106 kb on - strand, within alkAat 2927.155 kb on + strand, within alkAat 2927.166 kb on + strand, within alkAat 2927.166 kb on + strand, within alkAat 2927.166 kb on + strand, within alkAat 2927.187 kb on + strand, within alkAat 2927.194 kb on - strand, within alkAat 2927.206 kb on - strand, within alkAat 2927.263 kb on + strand, within alkAat 2927.335 kb on + strand, within alkAat 2927.335 kb on + strand, within alkAat 2927.336 kb on - strand, within alkAat 2927.370 kb on + strand, within alkAat 2927.461 kb on + strand, within alkAat 2927.461 kb on + strand, within alkAat 2927.461 kb on + strand, within alkAat 2927.461 kb on + strand, within alkAat 2927.512 kb on - strand, within alkAat 2927.512 kb on - strand, within alkAat 2927.729 kb on - strand, within alkAat 2927.816 kb on - strandat 2927.816 kb on - strandat 2927.822 kb on + strandat 2927.825 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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2,924,542 + yegI BNILDI_14495 0.50 +0.4
2,924,738 + yegI BNILDI_14495 0.60 +0.8
2,924,739 - yegI BNILDI_14495 0.60 +0.6
2,924,811 - yegI BNILDI_14495 0.64 +1.2
2,924,816 - yegI BNILDI_14495 0.64 +0.3
2,924,873 + yegI BNILDI_14495 0.67 -0.9
2,924,875 + yegI BNILDI_14495 0.67 -0.2
2,924,876 - yegI BNILDI_14495 0.67 +0.9
2,924,938 - yegI BNILDI_14495 0.71 +0.7
2,925,453 + +0.1
2,925,875 - yegD BNILDI_14500 0.26 -0.6
2,926,272 - yegD BNILDI_14500 0.56 -0.5
2,926,586 + yegD BNILDI_14500 0.79 -0.0
2,926,586 + yegD BNILDI_14500 0.79 -0.4
2,926,586 + yegD BNILDI_14500 0.79 +0.4
2,926,586 + yegD BNILDI_14500 0.79 -0.6
2,926,586 + yegD BNILDI_14500 0.79 -0.5
2,926,587 - yegD BNILDI_14500 0.79 +0.1
2,926,653 + yegD BNILDI_14500 0.84 -2.5
2,926,673 - yegD BNILDI_14500 0.85 -0.2
2,926,801 + +0.2
2,926,866 + -0.7
2,926,867 - +1.0
2,926,878 - +0.8
2,926,975 + +0.7
2,926,976 - -1.3
2,927,009 + +0.6
2,927,009 + -0.2
2,927,034 - +0.2
2,927,105 + alkA BNILDI_14505 0.12 -0.6
2,927,105 + alkA BNILDI_14505 0.12 +0.4
2,927,106 - alkA BNILDI_14505 0.12 +0.1
2,927,155 + alkA BNILDI_14505 0.17 -0.3
2,927,166 + alkA BNILDI_14505 0.19 -0.6
2,927,166 + alkA BNILDI_14505 0.19 -0.2
2,927,166 + alkA BNILDI_14505 0.19 +0.5
2,927,187 + alkA BNILDI_14505 0.21 +0.8
2,927,194 - alkA BNILDI_14505 0.22 -1.1
2,927,206 - alkA BNILDI_14505 0.23 +0.1
2,927,263 + alkA BNILDI_14505 0.30 +0.4
2,927,335 + alkA BNILDI_14505 0.38 -0.4
2,927,335 + alkA BNILDI_14505 0.38 +0.2
2,927,336 - alkA BNILDI_14505 0.38 -2.0
2,927,370 + alkA BNILDI_14505 0.42 +0.2
2,927,461 + alkA BNILDI_14505 0.53 +2.3
2,927,461 + alkA BNILDI_14505 0.53 -0.7
2,927,461 + alkA BNILDI_14505 0.53 +0.7
2,927,461 + alkA BNILDI_14505 0.53 -0.1
2,927,512 - alkA BNILDI_14505 0.59 +0.6
2,927,512 - alkA BNILDI_14505 0.59 -1.6
2,927,729 - alkA BNILDI_14505 0.84 +0.2
2,927,816 - +0.6
2,927,816 - -0.7
2,927,822 + -0.2
2,927,825 - -0.4

Or see this region's nucleotide sequence