Strain Fitness in Escherichia coli ECRC62 around BNILDI_09250

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_09245 and proS are separated by 52 nucleotidesproS and tsaA are separated by 111 nucleotidestsaA and rcsF overlap by 4 nucleotides BNILDI_09245: BNILDI_09245 - YaeF family permuted papain-like enzyme, at 1,855,289 to 1,855,870 _09245 BNILDI_09250: proS - proline--tRNA ligase, at 1,855,923 to 1,857,641 proS BNILDI_09255: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,857,753 to 1,858,460 tsaA BNILDI_09260: rcsF - Rcs stress response system protein RcsF, at 1,858,457 to 1,858,861 rcsF Position (kb) 1855 1856 1857 1858Strain fitness (log2 ratio) -2 -1 0 1 2at 1855.111 kb on + strandat 1855.111 kb on + strandat 1855.112 kb on - strandat 1855.117 kb on - strandat 1855.205 kb on + strandat 1855.205 kb on + strandat 1855.206 kb on - strandat 1855.206 kb on - strandat 1855.376 kb on - strand, within BNILDI_09245at 1855.385 kb on - strand, within BNILDI_09245at 1855.408 kb on + strand, within BNILDI_09245at 1855.408 kb on + strand, within BNILDI_09245at 1855.408 kb on + strand, within BNILDI_09245at 1855.409 kb on - strand, within BNILDI_09245at 1855.409 kb on - strand, within BNILDI_09245at 1855.567 kb on + strand, within BNILDI_09245at 1855.567 kb on + strand, within BNILDI_09245at 1855.571 kb on - strand, within BNILDI_09245at 1855.691 kb on + strand, within BNILDI_09245at 1855.700 kb on + strand, within BNILDI_09245at 1855.766 kb on + strand, within BNILDI_09245at 1855.766 kb on + strand, within BNILDI_09245at 1855.766 kb on + strand, within BNILDI_09245at 1855.767 kb on - strand, within BNILDI_09245at 1855.799 kb on - strand, within BNILDI_09245at 1855.808 kb on - strand, within BNILDI_09245at 1855.874 kb on - strandat 1857.836 kb on - strand, within tsaAat 1857.860 kb on + strand, within tsaAat 1857.861 kb on - strand, within tsaAat 1857.892 kb on - strand, within tsaAat 1858.048 kb on + strand, within tsaAat 1858.157 kb on + strand, within tsaAat 1858.290 kb on + strand, within tsaAat 1858.291 kb on - strand, within tsaAat 1858.336 kb on + strand, within tsaAat 1858.337 kb on - strand, within tsaAat 1858.414 kb on + strandat 1858.415 kb on - strandat 1858.434 kb on + strandat 1858.435 kb on - strandat 1858.435 kb on - strandat 1858.475 kb on - strandat 1858.493 kb on + strandat 1858.493 kb on + strandat 1858.494 kb on - strandat 1858.506 kb on + strand, within rcsFat 1858.540 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.548 kb on - strand, within rcsFat 1858.631 kb on - strand, within rcsF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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1,855,111 + -2.3
1,855,111 + -0.0
1,855,112 - +1.3
1,855,117 - +0.6
1,855,205 + -1.7
1,855,205 + +1.4
1,855,206 - +0.8
1,855,206 - -1.2
1,855,376 - BNILDI_09245 0.15 -0.2
1,855,385 - BNILDI_09245 0.16 +0.1
1,855,408 + BNILDI_09245 0.20 +0.8
1,855,408 + BNILDI_09245 0.20 +0.2
1,855,408 + BNILDI_09245 0.20 +0.3
1,855,409 - BNILDI_09245 0.21 -0.8
1,855,409 - BNILDI_09245 0.21 -1.2
1,855,567 + BNILDI_09245 0.48 +0.7
1,855,567 + BNILDI_09245 0.48 +0.2
1,855,571 - BNILDI_09245 0.48 -0.1
1,855,691 + BNILDI_09245 0.69 -0.4
1,855,700 + BNILDI_09245 0.71 +0.2
1,855,766 + BNILDI_09245 0.82 +0.7
1,855,766 + BNILDI_09245 0.82 -1.6
1,855,766 + BNILDI_09245 0.82 +1.0
1,855,767 - BNILDI_09245 0.82 +0.1
1,855,799 - BNILDI_09245 0.88 -0.9
1,855,808 - BNILDI_09245 0.89 -1.2
1,855,874 - +0.5
1,857,836 - tsaA BNILDI_09255 0.12 +0.7
1,857,860 + tsaA BNILDI_09255 0.15 +0.6
1,857,861 - tsaA BNILDI_09255 0.15 +0.3
1,857,892 - tsaA BNILDI_09255 0.20 +0.6
1,858,048 + tsaA BNILDI_09255 0.42 +0.9
1,858,157 + tsaA BNILDI_09255 0.57 -0.2
1,858,290 + tsaA BNILDI_09255 0.76 -0.1
1,858,291 - tsaA BNILDI_09255 0.76 +0.6
1,858,336 + tsaA BNILDI_09255 0.82 -0.1
1,858,337 - tsaA BNILDI_09255 0.82 +0.9
1,858,414 + -0.2
1,858,415 - +0.1
1,858,434 + -1.2
1,858,435 - +2.3
1,858,435 - +0.5
1,858,475 - -1.9
1,858,493 + +0.2
1,858,493 + -0.1
1,858,494 - +0.2
1,858,506 + rcsF BNILDI_09260 0.12 +0.2
1,858,540 + rcsF BNILDI_09260 0.20 +0.0
1,858,547 + rcsF BNILDI_09260 0.22 -1.2
1,858,547 + rcsF BNILDI_09260 0.22 -1.3
1,858,547 + rcsF BNILDI_09260 0.22 +1.2
1,858,548 - rcsF BNILDI_09260 0.22 -1.2
1,858,631 - rcsF BNILDI_09260 0.43 +0.0

Or see this region's nucleotide sequence