Strain Fitness in Escherichia coli ECRC62 around BNILDI_04220

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntivbL and tisB are separated by 564 nucleotidestisB and ysdE are separated by 262 nucleotidesysdE and emrD are separated by 81 nucleotides BNILDI_04205: ivbL - ilvB operon leader peptide IvbL, at 835,226 to 835,324 ivbL BNILDI_04215: tisB - Small toxic protein TisB, at 835,889 to 835,978 tisB BNILDI_04220: ysdE - Protein YsdE, at 836,241 to 836,315 ysdE BNILDI_04225: emrD - multidrug efflux MFS transporter EmrD, at 836,397 to 837,581 emrD Position (kb) 836 837Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 835.296 kb on - strand, within ivbLat 835.318 kb on + strandat 835.350 kb on - strandat 835.355 kb on + strandat 835.361 kb on + strandat 835.362 kb on - strandat 835.518 kb on - strandat 835.520 kb on + strandat 835.662 kb on - strandat 835.664 kb on - strandat 835.675 kb on - strandat 835.687 kb on - strandat 835.717 kb on + strandat 835.718 kb on - strandat 835.778 kb on + strandat 835.778 kb on + strandat 835.778 kb on + strandat 835.849 kb on + strandat 835.850 kb on - strandat 835.889 kb on - strandat 835.889 kb on - strandat 835.889 kb on - strandat 836.028 kb on + strandat 836.029 kb on - strandat 836.032 kb on + strandat 836.033 kb on - strandat 836.152 kb on + strandat 836.153 kb on - strandat 836.242 kb on + strandat 836.262 kb on + strand, within ysdEat 836.296 kb on + strand, within ysdEat 836.299 kb on - strand, within ysdEat 836.343 kb on + strandat 836.363 kb on + strandat 836.364 kb on - strandat 836.368 kb on + strandat 836.369 kb on - strandat 836.369 kb on - strandat 836.425 kb on + strandat 836.437 kb on + strandat 836.438 kb on - strandat 836.440 kb on + strandat 836.475 kb on + strandat 836.475 kb on + strandat 836.475 kb on + strandat 836.476 kb on - strandat 836.476 kb on - strandat 836.476 kb on - strandat 836.550 kb on + strand, within emrDat 836.562 kb on + strand, within emrDat 836.563 kb on - strand, within emrDat 836.563 kb on - strand, within emrDat 836.563 kb on - strand, within emrDat 836.646 kb on - strand, within emrDat 836.767 kb on + strand, within emrDat 836.768 kb on - strand, within emrDat 836.890 kb on - strand, within emrDat 836.996 kb on + strand, within emrDat 836.996 kb on + strand, within emrDat 836.997 kb on - strand, within emrDat 836.997 kb on - strand, within emrDat 836.997 kb on - strand, within emrDat 837.030 kb on + strand, within emrDat 837.109 kb on + strand, within emrDat 837.123 kb on - strand, within emrDat 837.134 kb on + strand, within emrDat 837.134 kb on + strand, within emrDat 837.143 kb on + strand, within emrDat 837.230 kb on + strand, within emrD

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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835,296 - ivbL BNILDI_04205 0.71 +1.4
835,318 + -0.6
835,350 - -0.7
835,355 + -3.6
835,361 + -2.0
835,362 - -0.3
835,518 - -0.3
835,520 + -0.0
835,662 - +0.2
835,664 - +0.2
835,675 - +0.8
835,687 - +0.0
835,717 + -1.9
835,718 - +0.1
835,778 + +0.6
835,778 + -0.3
835,778 + -0.0
835,849 + +0.9
835,850 - -1.5
835,889 - -0.1
835,889 - +1.9
835,889 - -2.0
836,028 + -0.5
836,029 - -1.7
836,032 + -2.9
836,033 - -0.4
836,152 + -0.1
836,153 - -0.1
836,242 + -0.6
836,262 + ysdE BNILDI_04220 0.28 -1.1
836,296 + ysdE BNILDI_04220 0.73 -1.0
836,299 - ysdE BNILDI_04220 0.77 -1.1
836,343 + -1.6
836,363 + +0.1
836,364 - -1.2
836,368 + +0.6
836,369 - +0.0
836,369 - +0.3
836,425 + -1.8
836,437 + -0.3
836,438 - -3.1
836,440 + +0.7
836,475 + +0.6
836,475 + +0.7
836,475 + +0.1
836,476 - +0.2
836,476 - -1.1
836,476 - -3.2
836,550 + emrD BNILDI_04225 0.13 +0.9
836,562 + emrD BNILDI_04225 0.14 +0.5
836,563 - emrD BNILDI_04225 0.14 -0.2
836,563 - emrD BNILDI_04225 0.14 -0.2
836,563 - emrD BNILDI_04225 0.14 -0.2
836,646 - emrD BNILDI_04225 0.21 -1.3
836,767 + emrD BNILDI_04225 0.31 +1.4
836,768 - emrD BNILDI_04225 0.31 -0.0
836,890 - emrD BNILDI_04225 0.42 -1.5
836,996 + emrD BNILDI_04225 0.51 +0.6
836,996 + emrD BNILDI_04225 0.51 -1.7
836,997 - emrD BNILDI_04225 0.51 +0.2
836,997 - emrD BNILDI_04225 0.51 +0.6
836,997 - emrD BNILDI_04225 0.51 +0.0
837,030 + emrD BNILDI_04225 0.53 -0.1
837,109 + emrD BNILDI_04225 0.60 -2.6
837,123 - emrD BNILDI_04225 0.61 -2.2
837,134 + emrD BNILDI_04225 0.62 -0.4
837,134 + emrD BNILDI_04225 0.62 -1.2
837,143 + emrD BNILDI_04225 0.63 -1.5
837,230 + emrD BNILDI_04225 0.70 +2.1

Or see this region's nucleotide sequence