Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and tsaE are separated by 18 nucleotides tsaE and nnr overlap by 29 nucleotides nnr and queG are separated by 13 nucleotides
NOLOHH_04535: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 821,493 to 822,830
amiB
NOLOHH_04540: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 822,849 to 823,310
tsaE
NOLOHH_04545: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 823,282 to 824,814
nnr
NOLOHH_04550: queG - tRNA epoxyqueuosine(34) reductase QueG, at 824,828 to 825,967
queG
Position (kb) 823
824
825 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 822.306 kb on - strand, within amiB at 822.306 kb on - strand, within amiB at 822.306 kb on - strand, within amiB at 822.308 kb on + strand, within amiB at 822.308 kb on + strand, within amiB at 822.309 kb on - strand, within amiB at 822.327 kb on - strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.475 kb on + strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.476 kb on - strand, within amiB at 822.686 kb on - strand, within amiB at 822.702 kb on - strand at 822.737 kb on + strand at 822.749 kb on + strand at 822.823 kb on + strand at 822.823 kb on + strand at 822.823 kb on + strand at 822.824 kb on - strand at 822.824 kb on - strand at 823.336 kb on - strand at 823.349 kb on - strand at 823.489 kb on - strand, within nnr at 823.489 kb on - strand, within nnr at 823.491 kb on + strand, within nnr at 823.624 kb on + strand, within nnr at 823.624 kb on + strand, within nnr at 823.625 kb on - strand, within nnr at 823.645 kb on + strand, within nnr at 823.646 kb on - strand, within nnr at 823.646 kb on - strand, within nnr at 823.646 kb on - strand, within nnr at 823.646 kb on - strand, within nnr at 823.646 kb on - strand, within nnr at 823.682 kb on - strand, within nnr at 823.978 kb on + strand, within nnr at 823.997 kb on - strand, within nnr at 824.016 kb on + strand, within nnr at 824.023 kb on - strand, within nnr at 824.056 kb on + strand, within nnr at 824.057 kb on - strand, within nnr at 824.250 kb on + strand, within nnr at 824.250 kb on + strand, within nnr at 824.250 kb on + strand, within nnr at 824.251 kb on - strand, within nnr at 824.325 kb on + strand, within nnr at 824.359 kb on - strand, within nnr at 824.359 kb on - strand, within nnr at 824.359 kb on - strand, within nnr at 824.359 kb on - strand, within nnr at 824.372 kb on - strand, within nnr at 824.380 kb on - strand, within nnr at 824.491 kb on + strand, within nnr at 824.491 kb on + strand, within nnr at 824.600 kb on + strand, within nnr at 824.620 kb on - strand, within nnr at 824.714 kb on + strand at 824.714 kb on + strand at 824.715 kb on - strand at 824.715 kb on - strand at 824.721 kb on + strand at 824.722 kb on - strand at 824.722 kb on - strand at 824.722 kb on - strand at 824.793 kb on + strand at 824.794 kb on - strand at 824.794 kb on - strand at 824.798 kb on - strand at 824.798 kb on - strand at 824.817 kb on + strand at 824.817 kb on + strand at 824.817 kb on + strand at 824.817 kb on + strand at 824.818 kb on - strand at 824.905 kb on - strand at 824.905 kb on - strand at 824.908 kb on + strand at 824.908 kb on + strand at 824.909 kb on - strand at 824.909 kb on - strand at 824.909 kb on - strand at 824.909 kb on - strand at 824.909 kb on - strand at 825.005 kb on - strand, within queG at 825.031 kb on - strand, within queG at 825.071 kb on + strand, within queG at 825.071 kb on + strand, within queG at 825.302 kb on - strand, within queG at 825.314 kb on - strand, within queG at 825.314 kb on - strand, within queG at 825.314 kb on - strand, within queG at 825.461 kb on + strand, within queG at 825.461 kb on + strand, within queG at 825.461 kb on + strand, within queG at 825.463 kb on + strand, within queG at 825.463 kb on + strand, within queG at 825.464 kb on - strand, within queG at 825.464 kb on - strand, within queG at 825.464 kb on - strand, within queG at 825.464 kb on - strand, within queG at 825.485 kb on - strand, within queG at 825.491 kb on + strand, within queG at 825.554 kb on - strand, within queG at 825.554 kb on - strand, within queG at 825.558 kb on - strand, within queG at 825.558 kb on - strand, within queG at 825.580 kb on + strand, within queG at 825.580 kb on + strand, within queG at 825.581 kb on - strand, within queG at 825.581 kb on - strand, within queG at 825.581 kb on - strand, within queG at 825.600 kb on - strand, within queG at 825.637 kb on + strand, within queG at 825.670 kb on - strand, within queG at 825.727 kb on + strand, within queG at 825.728 kb on - strand, within queG at 825.751 kb on + strand, within queG at 825.751 kb on + strand, within queG at 825.751 kb on + strand, within queG at 825.754 kb on + strand, within queG at 825.768 kb on + strand, within queG
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 822,306 - amiB NOLOHH_04535 0.61 -0.1 822,306 - amiB NOLOHH_04535 0.61 +0.1 822,306 - amiB NOLOHH_04535 0.61 -1.4 822,308 + amiB NOLOHH_04535 0.61 -0.3 822,308 + amiB NOLOHH_04535 0.61 +0.3 822,309 - amiB NOLOHH_04535 0.61 -0.4 822,327 - amiB NOLOHH_04535 0.62 -0.3 822,475 + amiB NOLOHH_04535 0.73 -0.2 822,475 + amiB NOLOHH_04535 0.73 -1.4 822,475 + amiB NOLOHH_04535 0.73 -0.2 822,475 + amiB NOLOHH_04535 0.73 -0.0 822,475 + amiB NOLOHH_04535 0.73 +0.4 822,475 + amiB NOLOHH_04535 0.73 +2.6 822,475 + amiB NOLOHH_04535 0.73 -2.9 822,475 + amiB NOLOHH_04535 0.73 +0.6 822,475 + amiB NOLOHH_04535 0.73 +0.2 822,476 - amiB NOLOHH_04535 0.73 -0.6 822,476 - amiB NOLOHH_04535 0.73 -0.4 822,476 - amiB NOLOHH_04535 0.73 +0.4 822,476 - amiB NOLOHH_04535 0.73 +0.1 822,476 - amiB NOLOHH_04535 0.73 +1.0 822,476 - amiB NOLOHH_04535 0.73 +0.5 822,476 - amiB NOLOHH_04535 0.73 +0.3 822,476 - amiB NOLOHH_04535 0.73 +0.1 822,476 - amiB NOLOHH_04535 0.73 -0.0 822,476 - amiB NOLOHH_04535 0.73 -0.3 822,476 - amiB NOLOHH_04535 0.73 +0.5 822,476 - amiB NOLOHH_04535 0.73 +0.6 822,686 - amiB NOLOHH_04535 0.89 -0.4 822,702 - +0.5 822,737 + +0.2 822,749 + +1.4 822,823 + +0.5 822,823 + -0.3 822,823 + -1.4 822,824 - -0.3 822,824 - -0.5 823,336 - -4.6 823,349 - +0.4 823,489 - nnr NOLOHH_04545 0.14 -0.7 823,489 - nnr NOLOHH_04545 0.14 +0.1 823,491 + nnr NOLOHH_04545 0.14 +0.4 823,624 + nnr NOLOHH_04545 0.22 +0.0 823,624 + nnr NOLOHH_04545 0.22 +0.1 823,625 - nnr NOLOHH_04545 0.22 +0.1 823,645 + nnr NOLOHH_04545 0.24 -0.3 823,646 - nnr NOLOHH_04545 0.24 -0.3 823,646 - nnr NOLOHH_04545 0.24 +0.3 823,646 - nnr NOLOHH_04545 0.24 +0.5 823,646 - nnr NOLOHH_04545 0.24 -0.6 823,646 - nnr NOLOHH_04545 0.24 -1.1 823,682 - nnr NOLOHH_04545 0.26 +0.1 823,978 + nnr NOLOHH_04545 0.45 +0.2 823,997 - nnr NOLOHH_04545 0.47 +0.2 824,016 + nnr NOLOHH_04545 0.48 -0.2 824,023 - nnr NOLOHH_04545 0.48 -0.3 824,056 + nnr NOLOHH_04545 0.50 -0.1 824,057 - nnr NOLOHH_04545 0.51 -0.1 824,250 + nnr NOLOHH_04545 0.63 -0.0 824,250 + nnr NOLOHH_04545 0.63 -0.9 824,250 + nnr NOLOHH_04545 0.63 +0.6 824,251 - nnr NOLOHH_04545 0.63 +0.3 824,325 + nnr NOLOHH_04545 0.68 +0.6 824,359 - nnr NOLOHH_04545 0.70 -1.7 824,359 - nnr NOLOHH_04545 0.70 -1.6 824,359 - nnr NOLOHH_04545 0.70 +0.4 824,359 - nnr NOLOHH_04545 0.70 -1.0 824,372 - nnr NOLOHH_04545 0.71 -0.3 824,380 - nnr NOLOHH_04545 0.72 +0.2 824,491 + nnr NOLOHH_04545 0.79 -0.2 824,491 + nnr NOLOHH_04545 0.79 -0.4 824,600 + nnr NOLOHH_04545 0.86 +2.5 824,620 - nnr NOLOHH_04545 0.87 -0.7 824,714 + +0.0 824,714 + -1.2 824,715 - +0.3 824,715 - -0.7 824,721 + -0.5 824,722 - -1.9 824,722 - +0.1 824,722 - -0.4 824,793 + +0.3 824,794 - +0.3 824,794 - +0.4 824,798 - +0.3 824,798 - +0.2 824,817 + -2.1 824,817 + -0.2 824,817 + -0.1 824,817 + +0.6 824,818 - +0.4 824,905 - +0.7 824,905 - -0.7 824,908 + +0.5 824,908 + -0.2 824,909 - -0.3 824,909 - -1.2 824,909 - +0.0 824,909 - +1.0 824,909 - -0.5 825,005 - queG NOLOHH_04550 0.16 -0.3 825,031 - queG NOLOHH_04550 0.18 -0.7 825,071 + queG NOLOHH_04550 0.21 +0.0 825,071 + queG NOLOHH_04550 0.21 +1.0 825,302 - queG NOLOHH_04550 0.42 -1.5 825,314 - queG NOLOHH_04550 0.43 +0.3 825,314 - queG NOLOHH_04550 0.43 +0.2 825,314 - queG NOLOHH_04550 0.43 +0.2 825,461 + queG NOLOHH_04550 0.56 +0.2 825,461 + queG NOLOHH_04550 0.56 +0.6 825,461 + queG NOLOHH_04550 0.56 -0.8 825,463 + queG NOLOHH_04550 0.56 -0.5 825,463 + queG NOLOHH_04550 0.56 -0.5 825,464 - queG NOLOHH_04550 0.56 +0.6 825,464 - queG NOLOHH_04550 0.56 -0.0 825,464 - queG NOLOHH_04550 0.56 +1.8 825,464 - queG NOLOHH_04550 0.56 +0.1 825,485 - queG NOLOHH_04550 0.58 -1.6 825,491 + queG NOLOHH_04550 0.58 +0.4 825,554 - queG NOLOHH_04550 0.64 -0.2 825,554 - queG NOLOHH_04550 0.64 -0.3 825,558 - queG NOLOHH_04550 0.64 +0.8 825,558 - queG NOLOHH_04550 0.64 -0.2 825,580 + queG NOLOHH_04550 0.66 +0.1 825,580 + queG NOLOHH_04550 0.66 -0.3 825,581 - queG NOLOHH_04550 0.66 -0.1 825,581 - queG NOLOHH_04550 0.66 +0.4 825,581 - queG NOLOHH_04550 0.66 -0.3 825,600 - queG NOLOHH_04550 0.68 -0.0 825,637 + queG NOLOHH_04550 0.71 +0.6 825,670 - queG NOLOHH_04550 0.74 -0.4 825,727 + queG NOLOHH_04550 0.79 +0.4 825,728 - queG NOLOHH_04550 0.79 +0.1 825,751 + queG NOLOHH_04550 0.81 +0.4 825,751 + queG NOLOHH_04550 0.81 +0.3 825,751 + queG NOLOHH_04550 0.81 +0.4 825,754 + queG NOLOHH_04550 0.81 -0.4 825,768 + queG NOLOHH_04550 0.82 -0.5
Or see this region's nucleotide sequence