Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28185

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntompT and hipB are separated by 498 nucleotideshipB and tnp-IS3 are separated by 173 nucleotidestnp-IS3 and tnp-IS3 overlap by 1 nucleotides OKFHMN_28180: ompT - omptin family outer membrane protease OmpT, at 5,408,478 to 5,409,431 ompT OKFHMN_28185: hipB - transcriptional regulator, at 5,409,930 to 5,410,514 hipB OKFHMN_28190: tnp-IS3 - IS3 family transposase, at 5,410,688 to 5,411,014 tnp-IS3 OKFHMN_28195: tnp-IS3 - IS3 family transposase, at 5,411,014 to 5,411,901 tnp-IS3 Position (kb) 5409 5410 5411Strain fitness (log2 ratio) -2 -1 0 1 2at 5408.954 kb on + strand, within ompTat 5408.955 kb on - strand, within ompTat 5409.132 kb on - strand, within ompTat 5409.219 kb on - strand, within ompTat 5409.337 kb on - strandat 5409.351 kb on - strandat 5409.736 kb on + strandat 5409.771 kb on + strandat 5409.878 kb on + strandat 5410.103 kb on + strand, within hipBat 5410.180 kb on + strand, within hipBat 5410.482 kb on + strandat 5410.550 kb on + strandat 5410.563 kb on + strandat 5410.563 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,408,954 + ompT OKFHMN_28180 0.50 -2.5
5,408,955 - ompT OKFHMN_28180 0.50 +0.3
5,409,132 - ompT OKFHMN_28180 0.69 +1.2
5,409,219 - ompT OKFHMN_28180 0.78 +1.1
5,409,337 - +0.3
5,409,351 - -2.3
5,409,736 + -1.8
5,409,771 + -1.3
5,409,878 + +0.5
5,410,103 + hipB OKFHMN_28185 0.30 +2.0
5,410,180 + hipB OKFHMN_28185 0.43 -0.9
5,410,482 + +1.5
5,410,550 + +0.7
5,410,563 + -0.3
5,410,563 + +1.4

Or see this region's nucleotide sequence