Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27740

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfL and mlc are separated by 134 nucleotidesmlc and bioD are separated by 124 nucleotidesbioD and clcB overlap by 48 nucleotides OKFHMN_27735: ynfL - Uncharacterized HTH-type transcriptional regulator YnfL, at 5,341,027 to 5,341,920 ynfL OKFHMN_27740: mlc - DNA-binding transcriptional repressor Mlc, at 5,342,055 to 5,343,275 mlc OKFHMN_27745: bioD - dethiobiotin synthase, at 5,343,400 to 5,344,095 bioD OKFHMN_27750: clcB - voltage-gated ClC-type chloride channel ClcB, at 5,344,048 to 5,345,304 clcB Position (kb) 5342 5343 5344Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5341.196 kb on + strand, within ynfLat 5341.251 kb on + strand, within ynfLat 5341.578 kb on - strand, within ynfLat 5342.522 kb on + strand, within mlcat 5342.628 kb on - strand, within mlcat 5343.179 kb on + strandat 5344.039 kb on + strandat 5344.120 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,341,196 + ynfL OKFHMN_27735 0.19 -1.3
5,341,251 + ynfL OKFHMN_27735 0.25 -0.0
5,341,578 - ynfL OKFHMN_27735 0.62 -4.1
5,342,522 + mlc OKFHMN_27740 0.38 -0.7
5,342,628 - mlc OKFHMN_27740 0.47 +0.3
5,343,179 + +0.2
5,344,039 + +0.3
5,344,120 + -0.6

Or see this region's nucleotide sequence