Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtJ and mdtI overlap by 14 nucleotides mdtI and ydgD are separated by 38 nucleotides ydgD and ydgU are separated by 99 nucleotides ydgU and asr are separated by 92 nucleotides
OKFHMN_27705: mdtJ - multidrug/spermidine efflux SMR transporter subunit MdtJ, at 5,337,118 to 5,337,483
mdtJ
OKFHMN_27710: mdtI - multidrug/spermidine efflux SMR transporter subunit MdtI, at 5,337,470 to 5,337,799
mdtI
OKFHMN_27715: ydgD - Uncharacterized serine protease YdgD, at 5,337,838 to 5,338,659
ydgD
OKFHMN_27720: ydgU - Uncharacterized protein YdgU, at 5,338,759 to 5,338,842
ydgU
OKFHMN_27725: asr - acid resistance repetitive basic protein Asr, at 5,338,935 to 5,339,243
asr
Position (kb) 5337
5338
5339 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5336.903 kb on + strand at 5336.932 kb on + strand at 5337.165 kb on + strand, within mdtJ at 5338.074 kb on - strand, within ydgD at 5338.203 kb on + strand, within ydgD at 5338.313 kb on - strand, within ydgD at 5338.653 kb on + strand at 5338.654 kb on - strand at 5338.677 kb on - strand at 5338.705 kb on + strand at 5338.712 kb on + strand at 5338.714 kb on + strand at 5338.754 kb on + strand at 5338.842 kb on + strand at 5338.882 kb on + strand at 5339.117 kb on + strand, within asr at 5339.301 kb on + strand at 5339.302 kb on - strand at 5339.355 kb on + strand at 5339.496 kb on + strand at 5339.496 kb on + strand at 5339.537 kb on + strand at 5339.537 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,336,903 + -0.1 5,336,932 + -0.2 5,337,165 + mdtJ OKFHMN_27705 0.13 +0.9 5,338,074 - ydgD OKFHMN_27715 0.29 +0.0 5,338,203 + ydgD OKFHMN_27715 0.44 +0.0 5,338,313 - ydgD OKFHMN_27715 0.58 -0.3 5,338,653 + -3.2 5,338,654 - -0.9 5,338,677 - +1.2 5,338,705 + -0.5 5,338,712 + -0.0 5,338,714 + +0.6 5,338,754 + +0.7 5,338,842 + +0.9 5,338,882 + -1.0 5,339,117 + asr OKFHMN_27725 0.59 +0.2 5,339,301 + +0.4 5,339,302 - +0.5 5,339,355 + +0.1 5,339,496 + +0.5 5,339,496 + -0.0 5,339,537 + +0.3 5,339,537 + -0.1
Or see this region's nucleotide sequence