Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27500

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmliC and pdxH are separated by 58 nucleotidespdxH and tyrS are separated by 128 nucleotidestyrS and pdxY are separated by 61 nucleotidespdxY and gstA are separated by 43 nucleotides OKFHMN_27490: mliC - C-type lysozyme inhibitor, at 5,292,304 to 5,292,627 mliC OKFHMN_27495: pdxH - pyridoxamine 5'-phosphate oxidase, at 5,292,686 to 5,293,342 pdxH OKFHMN_27500: tyrS - tyrosine--tRNA ligase, at 5,293,471 to 5,294,745 tyrS OKFHMN_27505: pdxY - pyridoxal kinase PdxY, at 5,294,807 to 5,295,667 pdxY OKFHMN_27510: gstA - glutathione transferase GstA, at 5,295,711 to 5,296,316 gstA Position (kb) 5293 5294 5295Strain fitness (log2 ratio) -2 -1 0 1at 5293.399 kb on + strandat 5293.399 kb on + strandat 5293.454 kb on + strandat 5293.456 kb on + strandat 5294.743 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,293,399 + -2.5
5,293,399 + -1.7
5,293,454 + +0.4
5,293,456 + -1.2
5,294,743 + -0.6

Or see this region's nucleotide sequence