Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27495

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntanmK and mliC are separated by 103 nucleotidesmliC and pdxH are separated by 58 nucleotidespdxH and tyrS are separated by 128 nucleotides OKFHMN_27485: anmK - anhydro-N-acetylmuramic acid kinase, at 5,291,091 to 5,292,200 anmK OKFHMN_27490: mliC - C-type lysozyme inhibitor, at 5,292,304 to 5,292,627 mliC OKFHMN_27495: pdxH - pyridoxamine 5'-phosphate oxidase, at 5,292,686 to 5,293,342 pdxH OKFHMN_27500: tyrS - tyrosine--tRNA ligase, at 5,293,471 to 5,294,745 tyrS Position (kb) 5292 5293 5294Strain fitness (log2 ratio) -2 -1 0 1at 5292.062 kb on + strand, within anmKat 5292.438 kb on + strand, within mliCat 5293.399 kb on + strandat 5293.399 kb on + strandat 5293.454 kb on + strandat 5293.456 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,292,062 + anmK OKFHMN_27485 0.87 -1.3
5,292,438 + mliC OKFHMN_27490 0.41 -1.5
5,293,399 + -2.5
5,293,399 + -1.7
5,293,454 + +0.4
5,293,456 + -1.2

Or see this region's nucleotide sequence