Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudG and ynjF are separated by 86 nucleotides ynjF and ynjE are separated by 8 nucleotides ynjE and ynjD are separated by 66 nucleotides ynjD and ynjC overlap by 1 nucleotides
OKFHMN_26850: nudG - CTP pyrophosphohydrolase, at 5,169,010 to 5,169,417
nudG
OKFHMN_26855: ynjF - Inner membrane protein YnjF, at 5,169,504 to 5,170,124
ynjF
OKFHMN_26860: ynjE - thiosulfate sulfurtransferase YnjE, at 5,170,133 to 5,171,440
ynjE
OKFHMN_26865: ynjD - Uncharacterized ABC transporter ATP-binding protein YnjD, at 5,171,507 to 5,172,160
ynjD
OKFHMN_26870: ynjC - Inner membrane ABC transporter permease protein YnjC, at 5,172,160 to 5,173,695
ynjC
Position (kb) 5170
5171
5172 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5169.222 kb on - strand, within nudG at 5169.222 kb on - strand, within nudG at 5169.486 kb on + strand at 5169.488 kb on + strand at 5170.009 kb on + strand, within ynjF at 5170.251 kb on + strand at 5170.336 kb on + strand, within ynjE at 5170.458 kb on + strand, within ynjE at 5170.869 kb on + strand, within ynjE at 5170.906 kb on + strand, within ynjE at 5171.074 kb on + strand, within ynjE at 5171.828 kb on - strand, within ynjD at 5171.828 kb on - strand, within ynjD at 5171.846 kb on - strand, within ynjD at 5172.086 kb on - strand, within ynjD at 5172.352 kb on - strand, within ynjC at 5172.354 kb on - strand, within ynjC at 5172.354 kb on - strand, within ynjC at 5172.364 kb on - strand, within ynjC at 5172.412 kb on - strand, within ynjC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,169,222 - nudG OKFHMN_26850 0.52 +0.4 5,169,222 - nudG OKFHMN_26850 0.52 +0.7 5,169,486 + +1.2 5,169,488 + +0.7 5,170,009 + ynjF OKFHMN_26855 0.81 -0.9 5,170,251 + -0.3 5,170,336 + ynjE OKFHMN_26860 0.16 -0.6 5,170,458 + ynjE OKFHMN_26860 0.25 -1.1 5,170,869 + ynjE OKFHMN_26860 0.56 -2.8 5,170,906 + ynjE OKFHMN_26860 0.59 -2.4 5,171,074 + ynjE OKFHMN_26860 0.72 -1.5 5,171,828 - ynjD OKFHMN_26865 0.49 +0.7 5,171,828 - ynjD OKFHMN_26865 0.49 -0.2 5,171,846 - ynjD OKFHMN_26865 0.52 -0.1 5,172,086 - ynjD OKFHMN_26865 0.89 -0.4 5,172,352 - ynjC OKFHMN_26870 0.12 +0.2 5,172,354 - ynjC OKFHMN_26870 0.13 +1.0 5,172,354 - ynjC OKFHMN_26870 0.13 +0.4 5,172,364 - ynjC OKFHMN_26870 0.13 +0.4 5,172,412 - ynjC OKFHMN_26870 0.16 -2.1
Or see this region's nucleotide sequence