Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and uvrC overlap by 4 nucleotides uvrC and pgsA are separated by 56 nucleotides pgsA and Gly_trna are separated by 151 nucleotides Gly_trna and Cys_trna are separated by 53 nucleotides Cys_trna and Leu_trna are separated by 12 nucleotides
OKFHMN_25820: uvrC - excinuclease ABC subunit UvrC, at 4,991,475 to 4,992,131
uvrC
OKFHMN_25825: uvrC - excinuclease ABC subunit UvrC, at 4,992,128 to 4,993,960
uvrC
OKFHMN_25830: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 4,994,017 to 4,994,565
pgsA
OKFHMN_25835: Gly_trna - tRNA-Gly(gcc), at 4,994,717 to 4,994,792
_trna
OKFHMN_25840: Cys_trna - tRNA-Cys(gca), at 4,994,846 to 4,994,919
_trna
OKFHMN_25845: Leu_trna - tRNA-Leu(taa), at 4,994,932 to 4,995,018
_trna
Position (kb) 4992
4993
4994 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4991.137 kb on + strand at 4991.149 kb on + strand at 4991.153 kb on - strand at 4991.290 kb on - strand at 4991.333 kb on + strand at 4991.543 kb on + strand, within uvrC at 4991.545 kb on + strand, within uvrC at 4991.594 kb on + strand, within uvrC at 4991.717 kb on + strand, within uvrC at 4991.740 kb on + strand, within uvrC at 4992.024 kb on + strand, within uvrC at 4992.035 kb on + strand, within uvrC at 4992.221 kb on + strand at 4992.222 kb on - strand at 4992.264 kb on - strand at 4992.330 kb on + strand, within uvrC at 4992.439 kb on + strand, within uvrC at 4992.509 kb on + strand, within uvrC at 4992.533 kb on + strand, within uvrC at 4992.633 kb on - strand, within uvrC at 4992.972 kb on - strand, within uvrC at 4993.037 kb on + strand, within uvrC at 4993.041 kb on + strand, within uvrC at 4993.333 kb on + strand, within uvrC at 4993.412 kb on - strand, within uvrC at 4993.412 kb on - strand, within uvrC at 4993.498 kb on + strand, within uvrC at 4993.498 kb on + strand, within uvrC at 4993.851 kb on - strand at 4994.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,991,137 + -3.1 4,991,149 + -1.1 4,991,153 - -1.5 4,991,290 - -0.7 4,991,333 + -1.2 4,991,543 + uvrC OKFHMN_25820 0.10 -0.0 4,991,545 + uvrC OKFHMN_25820 0.11 +0.7 4,991,594 + uvrC OKFHMN_25820 0.18 -1.7 4,991,717 + uvrC OKFHMN_25820 0.37 -0.1 4,991,740 + uvrC OKFHMN_25820 0.40 -0.6 4,992,024 + uvrC OKFHMN_25820 0.84 +1.2 4,992,035 + uvrC OKFHMN_25820 0.85 -2.5 4,992,221 + -2.1 4,992,222 - +2.3 4,992,264 - +1.2 4,992,330 + uvrC OKFHMN_25825 0.11 -1.5 4,992,439 + uvrC OKFHMN_25825 0.17 -0.4 4,992,509 + uvrC OKFHMN_25825 0.21 +1.7 4,992,533 + uvrC OKFHMN_25825 0.22 +1.2 4,992,633 - uvrC OKFHMN_25825 0.28 -0.9 4,992,972 - uvrC OKFHMN_25825 0.46 +0.9 4,993,037 + uvrC OKFHMN_25825 0.50 -0.6 4,993,041 + uvrC OKFHMN_25825 0.50 -3.4 4,993,333 + uvrC OKFHMN_25825 0.66 +0.9 4,993,412 - uvrC OKFHMN_25825 0.70 -2.4 4,993,412 - uvrC OKFHMN_25825 0.70 -0.2 4,993,498 + uvrC OKFHMN_25825 0.75 +1.7 4,993,498 + uvrC OKFHMN_25825 0.75 -1.7 4,993,851 - -0.1 4,994,683 + -0.5
Or see this region's nucleotide sequence