Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amyA and fliT are separated by 77 nucleotides fliT and fliS overlap by 1 nucleotides fliS and fliD are separated by 24 nucleotides
OKFHMN_25755: amyA - alpha-amylase, at 4,978,940 to 4,980,427
amyA
OKFHMN_25760: fliT - flagella biosynthesis regulatory protein FliT, at 4,980,505 to 4,980,870
fliT
OKFHMN_25765: fliS - flagellar export chaperone FliS, at 4,980,870 to 4,981,280
fliS
OKFHMN_25770: fliD - flagellar filament capping protein FliD, at 4,981,305 to 4,982,702
fliD
Position (kb)
4980
4981 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4979.597 kb on - strand, within amyA at 4979.599 kb on - strand, within amyA at 4979.645 kb on - strand, within amyA at 4979.711 kb on - strand, within amyA at 4980.007 kb on - strand, within amyA at 4980.152 kb on - strand, within amyA at 4980.203 kb on - strand, within amyA at 4980.245 kb on + strand, within amyA at 4980.295 kb on - strand at 4980.421 kb on + strand at 4980.626 kb on - strand, within fliT at 4980.629 kb on - strand, within fliT at 4980.629 kb on - strand, within fliT at 4980.637 kb on + strand, within fliT at 4980.747 kb on + strand, within fliT at 4980.930 kb on - strand, within fliS at 4981.007 kb on + strand, within fliS at 4981.008 kb on - strand, within fliS at 4981.009 kb on + strand, within fliS at 4981.014 kb on - strand, within fliS at 4981.016 kb on - strand, within fliS at 4981.039 kb on - strand, within fliS at 4981.276 kb on + strand at 4981.341 kb on + strand at 4981.342 kb on - strand at 4981.447 kb on + strand, within fliD at 4981.594 kb on - strand, within fliD at 4981.790 kb on + strand, within fliD at 4981.854 kb on + strand, within fliD at 4981.854 kb on + strand, within fliD at 4981.868 kb on - strand, within fliD at 4981.868 kb on - strand, within fliD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,979,597 - amyA OKFHMN_25755 0.44 +0.5 4,979,599 - amyA OKFHMN_25755 0.44 +1.1 4,979,645 - amyA OKFHMN_25755 0.47 +0.8 4,979,711 - amyA OKFHMN_25755 0.52 -0.5 4,980,007 - amyA OKFHMN_25755 0.72 -0.1 4,980,152 - amyA OKFHMN_25755 0.81 +0.7 4,980,203 - amyA OKFHMN_25755 0.85 +0.2 4,980,245 + amyA OKFHMN_25755 0.88 +0.7 4,980,295 - +0.4 4,980,421 + -3.8 4,980,626 - fliT OKFHMN_25760 0.33 +0.9 4,980,629 - fliT OKFHMN_25760 0.34 -0.8 4,980,629 - fliT OKFHMN_25760 0.34 -0.4 4,980,637 + fliT OKFHMN_25760 0.36 +3.4 4,980,747 + fliT OKFHMN_25760 0.66 -0.2 4,980,930 - fliS OKFHMN_25765 0.15 +0.1 4,981,007 + fliS OKFHMN_25765 0.33 -0.3 4,981,008 - fliS OKFHMN_25765 0.34 +0.2 4,981,009 + fliS OKFHMN_25765 0.34 +1.4 4,981,014 - fliS OKFHMN_25765 0.35 +0.3 4,981,016 - fliS OKFHMN_25765 0.36 +0.3 4,981,039 - fliS OKFHMN_25765 0.41 +0.9 4,981,276 + -1.5 4,981,341 + -0.9 4,981,342 - +2.1 4,981,447 + fliD OKFHMN_25770 0.10 -0.6 4,981,594 - fliD OKFHMN_25770 0.21 -0.2 4,981,790 + fliD OKFHMN_25770 0.35 +0.4 4,981,854 + fliD OKFHMN_25770 0.39 -2.1 4,981,854 + fliD OKFHMN_25770 0.39 +0.2 4,981,868 - fliD OKFHMN_25770 0.40 -0.2 4,981,868 - fliD OKFHMN_25770 0.40 -1.5
Or see this region's nucleotide sequence