Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25205

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_25195 and OKFHMN_25200 are separated by 13 nucleotidesOKFHMN_25200 and Asn_trna are separated by 123 nucleotidesAsn_trna and mtfA are separated by 100 nucleotidesmtfA and Ser_trna are separated by 93 nucleotides OKFHMN_25195: OKFHMN_25195 - Ig-like domain-containing protein, at 4,889,638 to 4,897,647 _25195 OKFHMN_25200: OKFHMN_25200 - hypothetical protein, at 4,897,661 to 4,897,810 _25200 OKFHMN_25205: Asn_trna - tRNA-Asn(gtt), at 4,897,934 to 4,898,009 _trna OKFHMN_25210: mtfA - DgsA anti-repressor MtfA, at 4,898,110 to 4,898,907 mtfA OKFHMN_25215: Ser_trna - tRNA-Ser(cga), at 4,899,001 to 4,899,090 _trna Position (kb) 4897 4898 4899Strain fitness (log2 ratio) -1 0 1at 4896.955 kb on - strandat 4897.409 kb on + strandat 4897.520 kb on - strandat 4897.624 kb on + strandat 4897.848 kb on - strandat 4897.856 kb on - strandat 4898.297 kb on + strand, within mtfAat 4898.297 kb on + strand, within mtfAat 4898.695 kb on + strand, within mtfAat 4898.884 kb on + strandat 4898.901 kb on + strandat 4898.901 kb on + strandat 4898.901 kb on + strandat 4898.902 kb on - strandat 4898.961 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,896,955 - +0.0
4,897,409 + -0.6
4,897,520 - +1.6
4,897,624 + -1.7
4,897,848 - -0.0
4,897,856 - -0.4
4,898,297 + mtfA OKFHMN_25210 0.23 -0.3
4,898,297 + mtfA OKFHMN_25210 0.23 -0.3
4,898,695 + mtfA OKFHMN_25210 0.73 +0.5
4,898,884 + +0.4
4,898,901 + -0.2
4,898,901 + -0.6
4,898,901 + +0.2
4,898,902 - +0.1
4,898,961 - -0.3

Or see this region's nucleotide sequence