Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24425

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotideswzx and rfbE are separated by 2 nucleotides OKFHMN_24415: wzy - O157 family O-antigen polymerase, at 4,769,299 to 4,770,483 wzy OKFHMN_24420: wcaA - glycosyl transferase, at 4,770,452 to 4,771,198 wcaA OKFHMN_24425: wzx - O157 family O-antigen flippase, at 4,771,185 to 4,772,576 wzx OKFHMN_24430: rfbE - GDP-perosamine synthase RfbE/PerA, at 4,772,579 to 4,773,673 rfbE Position (kb) 4771 4772 4773Strain fitness (log2 ratio) -1 0 1 2at 4770.247 kb on + strand, within wzyat 4770.429 kb on + strandat 4771.184 kb on + strandat 4772.827 kb on + strand, within rfbEat 4772.829 kb on + strand, within rfbEat 4773.247 kb on - strand, within rfbEat 4773.285 kb on + strand, within rfbE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,770,247 + wzy OKFHMN_24415 0.80 +1.9
4,770,429 + +1.2
4,771,184 + -0.2
4,772,827 + rfbE OKFHMN_24430 0.23 +1.1
4,772,829 + rfbE OKFHMN_24430 0.23 +0.5
4,773,247 - rfbE OKFHMN_24430 0.61 +0.8
4,773,285 + rfbE OKFHMN_24430 0.64 +0.5

Or see this region's nucleotide sequence