Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt udk and dcd are separated by 91 nucleotides dcd and asmA are separated by 21 nucleotides asmA and OKFHMN_24285 are separated by 52 nucleotides OKFHMN_24285 and OKFHMN_24290 overlap by 11 nucleotides OKFHMN_24290 and tlyC are separated by 74 nucleotides
OKFHMN_24270: udk - uridine kinase, at 4,736,999 to 4,737,640
udk
OKFHMN_24275: dcd - dCTP deaminase, at 4,737,732 to 4,738,313
dcd
OKFHMN_24280: asmA - outer membrane assembly protein AsmA, at 4,738,335 to 4,740,188
asmA
OKFHMN_24285: OKFHMN_24285 - transcriptional regulator, at 4,740,241 to 4,740,531
_24285
OKFHMN_24290: OKFHMN_24290 - Addiction module toxin RelE, at 4,740,521 to 4,740,859
_24290
OKFHMN_24295: tlyC - Integral membrane protein TerC family, at 4,740,934 to 4,742,517
tlyC
Position (kb)
4738
4739
4740
4741 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4737.606 kb on + strand at 4737.638 kb on + strand at 4737.812 kb on + strand, within dcd at 4737.975 kb on + strand, within dcd at 4737.976 kb on - strand, within dcd at 4738.434 kb on + strand at 4738.436 kb on + strand at 4738.615 kb on + strand, within asmA at 4739.042 kb on + strand, within asmA at 4739.042 kb on + strand, within asmA at 4739.111 kb on + strand, within asmA at 4739.130 kb on - strand, within asmA at 4739.351 kb on + strand, within asmA at 4739.650 kb on + strand, within asmA at 4740.091 kb on + strand at 4740.091 kb on + strand at 4740.196 kb on + strand at 4740.196 kb on + strand at 4740.198 kb on + strand at 4740.200 kb on + strand at 4740.237 kb on - strand at 4740.271 kb on - strand, within OKFHMN_24285 at 4740.280 kb on - strand, within OKFHMN_24285 at 4740.280 kb on - strand, within OKFHMN_24285 at 4740.280 kb on - strand, within OKFHMN_24285 at 4740.280 kb on - strand, within OKFHMN_24285 at 4740.284 kb on - strand, within OKFHMN_24285 at 4740.657 kb on + strand, within OKFHMN_24290 at 4740.658 kb on - strand, within OKFHMN_24290 at 4740.658 kb on - strand, within OKFHMN_24290 at 4740.658 kb on - strand, within OKFHMN_24290 at 4740.670 kb on - strand, within OKFHMN_24290 at 4740.976 kb on - strand at 4741.028 kb on + strand at 4741.140 kb on - strand, within tlyC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,737,606 + -0.1 4,737,638 + -0.9 4,737,812 + dcd OKFHMN_24275 0.14 -1.6 4,737,975 + dcd OKFHMN_24275 0.42 -2.1 4,737,976 - dcd OKFHMN_24275 0.42 -4.3 4,738,434 + -0.2 4,738,436 + -0.9 4,738,615 + asmA OKFHMN_24280 0.15 +0.4 4,739,042 + asmA OKFHMN_24280 0.38 +0.3 4,739,042 + asmA OKFHMN_24280 0.38 +0.6 4,739,111 + asmA OKFHMN_24280 0.42 -2.7 4,739,130 - asmA OKFHMN_24280 0.43 -1.8 4,739,351 + asmA OKFHMN_24280 0.55 -0.5 4,739,650 + asmA OKFHMN_24280 0.71 +0.5 4,740,091 + +0.4 4,740,091 + -0.3 4,740,196 + +0.9 4,740,196 + -1.7 4,740,198 + +0.5 4,740,200 + +0.5 4,740,237 - +0.4 4,740,271 - OKFHMN_24285 0.10 +0.2 4,740,280 - OKFHMN_24285 0.13 +1.2 4,740,280 - OKFHMN_24285 0.13 +0.9 4,740,280 - OKFHMN_24285 0.13 +0.0 4,740,280 - OKFHMN_24285 0.13 +1.0 4,740,284 - OKFHMN_24285 0.15 +0.2 4,740,657 + OKFHMN_24290 0.40 +1.1 4,740,658 - OKFHMN_24290 0.40 +0.3 4,740,658 - OKFHMN_24290 0.40 -0.1 4,740,658 - OKFHMN_24290 0.40 -0.6 4,740,670 - OKFHMN_24290 0.44 +0.9 4,740,976 - -0.4 4,741,028 + -0.6 4,741,140 - tlyC OKFHMN_24295 0.13 +0.1
Or see this region's nucleotide sequence