Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpC and glpB overlap by 4 nucleotides glpB and glpA overlap by 11 nucleotides
OKFHMN_23050: glpC - anaerobic glycerol-3-phosphate dehydrogenase subunit GlpC, at 4,487,274 to 4,488,464
glpC
OKFHMN_23055: glpB - glycerol-3-phosphate dehydrogenase subunit GlpB, at 4,488,461 to 4,489,720
glpB
OKFHMN_23060: glpA - anaerobic glycerol-3-phosphate dehydrogenase subunit A, at 4,489,710 to 4,491,338
glpA
Position (kb)
4488
4489
4490 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4487.733 kb on - strand, within glpC at 4487.769 kb on + strand, within glpC at 4487.797 kb on - strand, within glpC at 4487.887 kb on - strand, within glpC at 4487.919 kb on + strand, within glpC at 4487.928 kb on - strand, within glpC at 4487.948 kb on - strand, within glpC at 4487.988 kb on - strand, within glpC at 4488.046 kb on + strand, within glpC at 4488.217 kb on + strand, within glpC at 4488.301 kb on + strand, within glpC at 4488.301 kb on + strand, within glpC at 4488.302 kb on - strand, within glpC at 4488.319 kb on + strand, within glpC at 4488.434 kb on + strand at 4488.435 kb on - strand at 4488.507 kb on - strand at 4488.584 kb on - strand at 4488.584 kb on - strand at 4488.686 kb on - strand, within glpB at 4488.687 kb on + strand, within glpB at 4488.688 kb on - strand, within glpB at 4488.688 kb on - strand, within glpB at 4488.857 kb on + strand, within glpB at 4488.858 kb on - strand, within glpB at 4489.110 kb on - strand, within glpB at 4489.110 kb on - strand, within glpB at 4489.132 kb on + strand, within glpB at 4489.209 kb on - strand, within glpB at 4489.304 kb on + strand, within glpB at 4489.304 kb on + strand, within glpB at 4489.404 kb on - strand, within glpB at 4489.439 kb on + strand, within glpB at 4489.494 kb on + strand, within glpB at 4489.495 kb on - strand, within glpB at 4489.495 kb on - strand, within glpB at 4489.676 kb on + strand at 4489.770 kb on - strand at 4489.857 kb on - strand at 4489.880 kb on + strand, within glpA at 4489.962 kb on + strand, within glpA at 4489.977 kb on - strand, within glpA at 4490.040 kb on + strand, within glpA at 4490.201 kb on + strand, within glpA at 4490.267 kb on - strand, within glpA at 4490.297 kb on + strand, within glpA at 4490.298 kb on - strand, within glpA at 4490.408 kb on - strand, within glpA at 4490.471 kb on + strand, within glpA at 4490.515 kb on + strand, within glpA at 4490.516 kb on - strand, within glpA at 4490.526 kb on + strand, within glpA at 4490.526 kb on + strand, within glpA at 4490.526 kb on + strand, within glpA at 4490.527 kb on - strand, within glpA at 4490.527 kb on - strand, within glpA at 4490.527 kb on - strand, within glpA at 4490.618 kb on + strand, within glpA at 4490.619 kb on - strand, within glpA at 4490.619 kb on - strand, within glpA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,487,733 - glpC OKFHMN_23050 0.39 -0.8 4,487,769 + glpC OKFHMN_23050 0.42 -4.1 4,487,797 - glpC OKFHMN_23050 0.44 +0.0 4,487,887 - glpC OKFHMN_23050 0.51 +0.3 4,487,919 + glpC OKFHMN_23050 0.54 +1.0 4,487,928 - glpC OKFHMN_23050 0.55 +0.5 4,487,948 - glpC OKFHMN_23050 0.57 -1.4 4,487,988 - glpC OKFHMN_23050 0.60 +0.3 4,488,046 + glpC OKFHMN_23050 0.65 -0.2 4,488,217 + glpC OKFHMN_23050 0.79 -0.7 4,488,301 + glpC OKFHMN_23050 0.86 -1.9 4,488,301 + glpC OKFHMN_23050 0.86 -3.7 4,488,302 - glpC OKFHMN_23050 0.86 -0.8 4,488,319 + glpC OKFHMN_23050 0.88 +1.2 4,488,434 + -2.3 4,488,435 - +0.4 4,488,507 - +0.4 4,488,584 - -1.9 4,488,584 - +0.1 4,488,686 - glpB OKFHMN_23055 0.18 +0.6 4,488,687 + glpB OKFHMN_23055 0.18 -0.2 4,488,688 - glpB OKFHMN_23055 0.18 +0.1 4,488,688 - glpB OKFHMN_23055 0.18 +0.2 4,488,857 + glpB OKFHMN_23055 0.31 +1.3 4,488,858 - glpB OKFHMN_23055 0.32 +0.2 4,489,110 - glpB OKFHMN_23055 0.52 +0.3 4,489,110 - glpB OKFHMN_23055 0.52 +0.4 4,489,132 + glpB OKFHMN_23055 0.53 +0.3 4,489,209 - glpB OKFHMN_23055 0.59 -1.4 4,489,304 + glpB OKFHMN_23055 0.67 -0.7 4,489,304 + glpB OKFHMN_23055 0.67 +0.5 4,489,404 - glpB OKFHMN_23055 0.75 +0.0 4,489,439 + glpB OKFHMN_23055 0.78 -1.8 4,489,494 + glpB OKFHMN_23055 0.82 -3.6 4,489,495 - glpB OKFHMN_23055 0.82 +0.5 4,489,495 - glpB OKFHMN_23055 0.82 +0.4 4,489,676 + +2.0 4,489,770 - +2.5 4,489,857 - -0.8 4,489,880 + glpA OKFHMN_23060 0.10 +0.2 4,489,962 + glpA OKFHMN_23060 0.15 +0.7 4,489,977 - glpA OKFHMN_23060 0.16 +0.2 4,490,040 + glpA OKFHMN_23060 0.20 +1.9 4,490,201 + glpA OKFHMN_23060 0.30 +0.4 4,490,267 - glpA OKFHMN_23060 0.34 +0.8 4,490,297 + glpA OKFHMN_23060 0.36 +0.4 4,490,298 - glpA OKFHMN_23060 0.36 +0.6 4,490,408 - glpA OKFHMN_23060 0.43 -0.2 4,490,471 + glpA OKFHMN_23060 0.47 -0.7 4,490,515 + glpA OKFHMN_23060 0.49 +0.0 4,490,516 - glpA OKFHMN_23060 0.49 +0.7 4,490,526 + glpA OKFHMN_23060 0.50 +1.6 4,490,526 + glpA OKFHMN_23060 0.50 -0.3 4,490,526 + glpA OKFHMN_23060 0.50 +0.1 4,490,527 - glpA OKFHMN_23060 0.50 +0.8 4,490,527 - glpA OKFHMN_23060 0.50 +0.9 4,490,527 - glpA OKFHMN_23060 0.50 -1.1 4,490,618 + glpA OKFHMN_23060 0.56 -0.6 4,490,619 - glpA OKFHMN_23060 0.56 -0.4 4,490,619 - glpA OKFHMN_23060 0.56 +0.1
Or see this region's nucleotide sequence