Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menE and pmrD are separated by 109 nucleotides pmrD and arnF overlap by 7 nucleotides arnF and arnE overlap by 1 nucleotides arnE and arnT overlap by 4 nucleotides
OKFHMN_22955: menE - o-succinylbenzoate--CoA ligase, at 4,469,153 to 4,470,508
menE
OKFHMN_22960: pmrD - signal transduction protein PmrD, at 4,470,618 to 4,470,884
pmrD
OKFHMN_22965: arnF - 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF, at 4,470,878 to 4,471,264
arnF
OKFHMN_22970: arnE - 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE, at 4,471,264 to 4,471,599
arnE
OKFHMN_22975: arnT - lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase, at 4,471,596 to 4,473,248
arnT
Position (kb) 4470
4471
4472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4469.883 kb on + strand, within menE at 4469.884 kb on - strand, within menE at 4469.884 kb on - strand, within menE at 4469.884 kb on - strand, within menE at 4470.059 kb on - strand, within menE at 4470.146 kb on + strand, within menE at 4470.213 kb on + strand, within menE at 4470.214 kb on - strand, within menE at 4470.261 kb on + strand, within menE at 4470.507 kb on - strand at 4470.572 kb on + strand at 4470.614 kb on + strand at 4470.614 kb on + strand at 4470.659 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.670 kb on + strand, within pmrD at 4470.671 kb on - strand, within pmrD at 4470.674 kb on + strand, within pmrD at 4470.674 kb on + strand, within pmrD at 4470.674 kb on + strand, within pmrD at 4470.675 kb on - strand, within pmrD at 4470.751 kb on + strand, within pmrD at 4470.782 kb on + strand, within pmrD at 4470.783 kb on - strand, within pmrD at 4470.831 kb on + strand, within pmrD at 4470.833 kb on + strand, within pmrD at 4470.860 kb on + strand at 4470.860 kb on + strand at 4470.929 kb on - strand, within arnF at 4470.987 kb on - strand, within arnF at 4471.014 kb on + strand, within arnF at 4471.022 kb on - strand, within arnF at 4471.061 kb on + strand, within arnF at 4471.062 kb on - strand, within arnF at 4471.113 kb on - strand, within arnF at 4471.211 kb on + strand, within arnF at 4471.212 kb on - strand, within arnF at 4471.231 kb on + strand at 4471.266 kb on - strand at 4471.300 kb on - strand, within arnE at 4471.391 kb on - strand, within arnE at 4471.583 kb on - strand at 4471.609 kb on + strand at 4471.676 kb on + strand at 4471.709 kb on + strand at 4471.737 kb on - strand at 4471.793 kb on + strand, within arnT at 4471.883 kb on + strand, within arnT at 4472.049 kb on + strand, within arnT at 4472.111 kb on - strand, within arnT at 4472.111 kb on - strand, within arnT at 4472.178 kb on - strand, within arnT at 4472.205 kb on - strand, within arnT at 4472.260 kb on + strand, within arnT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,469,883 + menE OKFHMN_22955 0.54 +0.4 4,469,884 - menE OKFHMN_22955 0.54 -0.9 4,469,884 - menE OKFHMN_22955 0.54 +0.2 4,469,884 - menE OKFHMN_22955 0.54 -1.5 4,470,059 - menE OKFHMN_22955 0.67 +0.1 4,470,146 + menE OKFHMN_22955 0.73 +0.0 4,470,213 + menE OKFHMN_22955 0.78 -0.6 4,470,214 - menE OKFHMN_22955 0.78 -0.6 4,470,261 + menE OKFHMN_22955 0.82 +0.5 4,470,507 - +0.4 4,470,572 + -1.2 4,470,614 + -0.0 4,470,614 + +0.7 4,470,659 + pmrD OKFHMN_22960 0.15 -1.2 4,470,670 + pmrD OKFHMN_22960 0.19 -0.6 4,470,670 + pmrD OKFHMN_22960 0.19 -1.0 4,470,670 + pmrD OKFHMN_22960 0.19 +0.7 4,470,670 + pmrD OKFHMN_22960 0.19 +0.2 4,470,670 + pmrD OKFHMN_22960 0.19 +0.3 4,470,670 + pmrD OKFHMN_22960 0.19 +0.5 4,470,670 + pmrD OKFHMN_22960 0.19 +0.5 4,470,670 + pmrD OKFHMN_22960 0.19 +0.6 4,470,671 - pmrD OKFHMN_22960 0.20 -2.4 4,470,674 + pmrD OKFHMN_22960 0.21 +2.3 4,470,674 + pmrD OKFHMN_22960 0.21 -3.5 4,470,674 + pmrD OKFHMN_22960 0.21 -0.7 4,470,675 - pmrD OKFHMN_22960 0.21 -0.4 4,470,751 + pmrD OKFHMN_22960 0.50 -0.8 4,470,782 + pmrD OKFHMN_22960 0.61 +0.1 4,470,783 - pmrD OKFHMN_22960 0.62 +0.3 4,470,831 + pmrD OKFHMN_22960 0.80 -0.3 4,470,833 + pmrD OKFHMN_22960 0.81 +0.5 4,470,860 + -0.3 4,470,860 + +0.0 4,470,929 - arnF OKFHMN_22965 0.13 +1.3 4,470,987 - arnF OKFHMN_22965 0.28 -2.0 4,471,014 + arnF OKFHMN_22965 0.35 -1.4 4,471,022 - arnF OKFHMN_22965 0.37 -1.5 4,471,061 + arnF OKFHMN_22965 0.47 +1.3 4,471,062 - arnF OKFHMN_22965 0.48 -0.7 4,471,113 - arnF OKFHMN_22965 0.61 -0.0 4,471,211 + arnF OKFHMN_22965 0.86 +0.3 4,471,212 - arnF OKFHMN_22965 0.86 +0.5 4,471,231 + +1.2 4,471,266 - +0.6 4,471,300 - arnE OKFHMN_22970 0.11 +1.7 4,471,391 - arnE OKFHMN_22970 0.38 +0.2 4,471,583 - +2.2 4,471,609 + +1.2 4,471,676 + -1.1 4,471,709 + +0.8 4,471,737 - -0.3 4,471,793 + arnT OKFHMN_22975 0.12 +0.2 4,471,883 + arnT OKFHMN_22975 0.17 +1.0 4,472,049 + arnT OKFHMN_22975 0.27 +0.4 4,472,111 - arnT OKFHMN_22975 0.31 +0.7 4,472,111 - arnT OKFHMN_22975 0.31 +0.6 4,472,178 - arnT OKFHMN_22975 0.35 +0.0 4,472,205 - arnT OKFHMN_22975 0.37 +1.0 4,472,260 + arnT OKFHMN_22975 0.40 +0.6
Or see this region's nucleotide sequence