Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaC and ypdK are separated by 64 nucleotides ypdK and lpxP are separated by 370 nucleotides lpxP and yfdY are separated by 352 nucleotides yfdY and OKFHMN_22390 are separated by 76 nucleotides
OKFHMN_22370: alaC - alanine transaminase, at 4,348,152 to 4,349,390
alaC
OKFHMN_22375: ypdK - membrane protein YpdK, at 4,349,455 to 4,349,511
ypdK
OKFHMN_22380: lpxP - kdo(2)-lipid IV(A) palmitoleoyltransferase, at 4,349,882 to 4,350,802
lpxP
OKFHMN_22385: yfdY - Uncharacterized protein YfdY, at 4,351,155 to 4,351,397
yfdY
OKFHMN_22390: OKFHMN_22390 - Uncharacterized lipoprotein YpdI, at 4,351,474 to 4,351,749
_22390
Position (kb) 4349
4350
4351 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4349.058 kb on + strand, within alaC at 4349.078 kb on - strand, within alaC at 4349.163 kb on + strand, within alaC at 4349.298 kb on - strand at 4349.389 kb on - strand at 4349.497 kb on - strand, within ypdK at 4349.548 kb on - strand at 4349.582 kb on - strand at 4349.582 kb on - strand at 4349.582 kb on - strand at 4349.624 kb on - strand at 4349.992 kb on - strand, within lpxP at 4350.087 kb on - strand, within lpxP at 4350.126 kb on + strand, within lpxP at 4350.127 kb on - strand, within lpxP at 4350.286 kb on + strand, within lpxP at 4350.488 kb on - strand, within lpxP at 4350.492 kb on - strand, within lpxP at 4350.728 kb on + strand at 4350.767 kb on + strand at 4350.803 kb on - strand at 4350.806 kb on + strand at 4350.807 kb on - strand at 4350.815 kb on + strand at 4350.815 kb on + strand at 4350.816 kb on - strand at 4350.960 kb on + strand at 4350.965 kb on + strand at 4350.996 kb on + strand at 4351.154 kb on + strand at 4351.154 kb on + strand at 4351.162 kb on - strand at 4351.187 kb on + strand, within yfdY at 4351.187 kb on + strand, within yfdY at 4351.219 kb on + strand, within yfdY at 4351.281 kb on + strand, within yfdY at 4351.284 kb on + strand, within yfdY at 4351.300 kb on + strand, within yfdY at 4351.373 kb on + strand at 4351.395 kb on + strand at 4351.396 kb on - strand at 4351.543 kb on + strand, within OKFHMN_22390 at 4351.570 kb on - strand, within OKFHMN_22390 at 4351.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,349,058 + alaC OKFHMN_22370 0.73 +0.6 4,349,078 - alaC OKFHMN_22370 0.75 +0.5 4,349,163 + alaC OKFHMN_22370 0.82 +0.3 4,349,298 - -3.7 4,349,389 - +0.0 4,349,497 - ypdK OKFHMN_22375 0.74 -0.0 4,349,548 - +0.2 4,349,582 - +0.3 4,349,582 - -0.2 4,349,582 - +1.5 4,349,624 - -0.5 4,349,992 - lpxP OKFHMN_22380 0.12 +0.1 4,350,087 - lpxP OKFHMN_22380 0.22 +0.1 4,350,126 + lpxP OKFHMN_22380 0.26 +1.1 4,350,127 - lpxP OKFHMN_22380 0.27 +0.1 4,350,286 + lpxP OKFHMN_22380 0.44 +0.0 4,350,488 - lpxP OKFHMN_22380 0.66 +0.3 4,350,492 - lpxP OKFHMN_22380 0.66 +0.9 4,350,728 + -1.4 4,350,767 + -0.1 4,350,803 - -3.4 4,350,806 + +0.9 4,350,807 - -0.2 4,350,815 + -1.2 4,350,815 + +0.7 4,350,816 - +1.3 4,350,960 + +0.0 4,350,965 + -0.2 4,350,996 + -1.9 4,351,154 + +0.1 4,351,154 + +0.4 4,351,162 - +0.7 4,351,187 + yfdY OKFHMN_22385 0.13 -0.3 4,351,187 + yfdY OKFHMN_22385 0.13 -0.1 4,351,219 + yfdY OKFHMN_22385 0.26 +0.2 4,351,281 + yfdY OKFHMN_22385 0.52 -1.0 4,351,284 + yfdY OKFHMN_22385 0.53 +1.4 4,351,300 + yfdY OKFHMN_22385 0.60 -1.7 4,351,373 + -1.3 4,351,395 + +0.5 4,351,396 - +0.6 4,351,543 + OKFHMN_22390 0.25 +1.0 4,351,570 - OKFHMN_22390 0.35 -0.1 4,351,801 - +0.8
Or see this region's nucleotide sequence