Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22120

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeZ and yfeY are separated by 60 nucleotidesyfeY and yfeX are separated by 95 nucleotidesyfeX and pbp4b are separated by 57 nucleotides OKFHMN_22110: yfeZ - Inner membrane protein YfeZ, at 4,299,750 to 4,300,199 yfeZ OKFHMN_22115: yfeY - RpoE-regulated lipoprotein, at 4,300,260 to 4,300,835 yfeY OKFHMN_22120: yfeX - porphyrinogen peroxidase, at 4,300,931 to 4,301,830 yfeX OKFHMN_22125: pbp4b - penicillin binding protein PBP4B, at 4,301,888 to 4,303,192 pbp4b Position (kb) 4300 4301 4302Strain fitness (log2 ratio) -2 -1 0 1 2at 4299.995 kb on + strand, within yfeZat 4300.023 kb on + strand, within yfeZat 4300.054 kb on + strand, within yfeZat 4300.055 kb on - strand, within yfeZat 4300.120 kb on + strand, within yfeZat 4300.276 kb on + strandat 4300.276 kb on + strandat 4300.289 kb on + strandat 4300.332 kb on - strand, within yfeYat 4300.406 kb on - strand, within yfeYat 4300.691 kb on + strand, within yfeYat 4300.730 kb on + strand, within yfeYat 4300.731 kb on - strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.734 kb on - strand, within yfeYat 4300.734 kb on - strand, within yfeYat 4300.821 kb on - strandat 4300.912 kb on - strandat 4301.111 kb on - strand, within yfeXat 4301.698 kb on + strand, within yfeXat 4301.792 kb on + strandat 4301.793 kb on - strandat 4301.887 kb on - strandat 4301.890 kb on - strandat 4301.911 kb on - strandat 4301.925 kb on - strandat 4301.935 kb on - strandat 4301.935 kb on - strandat 4301.935 kb on - strandat 4301.968 kb on + strandat 4301.969 kb on - strandat 4302.035 kb on - strand, within pbp4bat 4302.475 kb on + strand, within pbp4bat 4302.476 kb on - strand, within pbp4bat 4302.499 kb on - strand, within pbp4bat 4302.568 kb on + strand, within pbp4bat 4302.568 kb on + strand, within pbp4bat 4302.570 kb on + strand, within pbp4bat 4302.571 kb on - strand, within pbp4bat 4302.585 kb on + strand, within pbp4bat 4302.586 kb on - strand, within pbp4bat 4302.586 kb on - strand, within pbp4bat 4302.589 kb on + strand, within pbp4bat 4302.589 kb on + strand, within pbp4bat 4302.590 kb on - strand, within pbp4bat 4302.610 kb on - strand, within pbp4bat 4302.610 kb on - strand, within pbp4bat 4302.663 kb on + strand, within pbp4bat 4302.664 kb on - strand, within pbp4bat 4302.664 kb on - strand, within pbp4bat 4302.665 kb on + strand, within pbp4bat 4302.665 kb on + strand, within pbp4bat 4302.666 kb on - strand, within pbp4b

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,299,995 + yfeZ OKFHMN_22110 0.54 -2.6
4,300,023 + yfeZ OKFHMN_22110 0.61 -0.7
4,300,054 + yfeZ OKFHMN_22110 0.68 -1.3
4,300,055 - yfeZ OKFHMN_22110 0.68 -1.5
4,300,120 + yfeZ OKFHMN_22110 0.82 +0.5
4,300,276 + -0.5
4,300,276 + -0.7
4,300,289 + -0.1
4,300,332 - yfeY OKFHMN_22115 0.12 -1.5
4,300,406 - yfeY OKFHMN_22115 0.25 +0.5
4,300,691 + yfeY OKFHMN_22115 0.75 +1.0
4,300,730 + yfeY OKFHMN_22115 0.82 -1.2
4,300,731 - yfeY OKFHMN_22115 0.82 +0.8
4,300,733 + yfeY OKFHMN_22115 0.82 +0.1
4,300,733 + yfeY OKFHMN_22115 0.82 +0.3
4,300,733 + yfeY OKFHMN_22115 0.82 -0.3
4,300,734 - yfeY OKFHMN_22115 0.82 +0.0
4,300,734 - yfeY OKFHMN_22115 0.82 +1.8
4,300,821 - -0.4
4,300,912 - -2.1
4,301,111 - yfeX OKFHMN_22120 0.20 +1.0
4,301,698 + yfeX OKFHMN_22120 0.85 +0.0
4,301,792 + +0.1
4,301,793 - -0.8
4,301,887 - +2.1
4,301,890 - +0.4
4,301,911 - -1.4
4,301,925 - -1.8
4,301,935 - +0.6
4,301,935 - +1.0
4,301,935 - +0.2
4,301,968 + +0.0
4,301,969 - +0.2
4,302,035 - pbp4b OKFHMN_22125 0.11 -0.4
4,302,475 + pbp4b OKFHMN_22125 0.45 +0.8
4,302,476 - pbp4b OKFHMN_22125 0.45 +1.3
4,302,499 - pbp4b OKFHMN_22125 0.47 +0.9
4,302,568 + pbp4b OKFHMN_22125 0.52 -1.6
4,302,568 + pbp4b OKFHMN_22125 0.52 +0.3
4,302,570 + pbp4b OKFHMN_22125 0.52 -0.6
4,302,571 - pbp4b OKFHMN_22125 0.52 -0.8
4,302,585 + pbp4b OKFHMN_22125 0.53 +0.3
4,302,586 - pbp4b OKFHMN_22125 0.53 -0.3
4,302,586 - pbp4b OKFHMN_22125 0.53 +0.1
4,302,589 + pbp4b OKFHMN_22125 0.54 -1.5
4,302,589 + pbp4b OKFHMN_22125 0.54 +0.3
4,302,590 - pbp4b OKFHMN_22125 0.54 -0.1
4,302,610 - pbp4b OKFHMN_22125 0.55 +0.8
4,302,610 - pbp4b OKFHMN_22125 0.55 -0.5
4,302,663 + pbp4b OKFHMN_22125 0.59 +0.2
4,302,664 - pbp4b OKFHMN_22125 0.59 -0.1
4,302,664 - pbp4b OKFHMN_22125 0.59 -0.3
4,302,665 + pbp4b OKFHMN_22125 0.60 +1.0
4,302,665 + pbp4b OKFHMN_22125 0.60 +0.3
4,302,666 - pbp4b OKFHMN_22125 0.60 +2.0

Or see this region's nucleotide sequence