Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiA and ypeA are separated by 213 nucleotides ypeA and yfeZ overlap by 14 nucleotides yfeZ and yfeY are separated by 60 nucleotides
OKFHMN_22100: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 4,298,255 to 4,299,124
amiA
OKFHMN_22105: ypeA - GNAT family acetyltransferase, at 4,299,338 to 4,299,763
ypeA
OKFHMN_22110: yfeZ - Inner membrane protein YfeZ, at 4,299,750 to 4,300,199
yfeZ
OKFHMN_22115: yfeY - RpoE-regulated lipoprotein, at 4,300,260 to 4,300,835
yfeY
Position (kb)
4299
4300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4298.530 kb on + strand, within amiA at 4298.714 kb on - strand, within amiA at 4298.837 kb on - strand, within amiA at 4299.009 kb on - strand, within amiA at 4299.138 kb on + strand at 4299.159 kb on + strand at 4299.160 kb on - strand at 4299.160 kb on - strand at 4299.162 kb on - strand at 4299.173 kb on + strand at 4299.210 kb on - strand at 4299.225 kb on - strand at 4299.334 kb on + strand at 4299.351 kb on + strand at 4299.445 kb on + strand, within ypeA at 4299.446 kb on - strand, within ypeA at 4299.548 kb on + strand, within ypeA at 4299.549 kb on - strand, within ypeA at 4299.549 kb on - strand, within ypeA at 4299.549 kb on - strand, within ypeA at 4299.805 kb on + strand, within yfeZ at 4299.890 kb on + strand, within yfeZ at 4299.912 kb on + strand, within yfeZ at 4299.995 kb on + strand, within yfeZ at 4300.023 kb on + strand, within yfeZ at 4300.054 kb on + strand, within yfeZ at 4300.055 kb on - strand, within yfeZ at 4300.120 kb on + strand, within yfeZ at 4300.276 kb on + strand at 4300.276 kb on + strand at 4300.289 kb on + strand at 4300.332 kb on - strand, within yfeY at 4300.406 kb on - strand, within yfeY at 4300.691 kb on + strand, within yfeY at 4300.730 kb on + strand, within yfeY at 4300.731 kb on - strand, within yfeY at 4300.733 kb on + strand, within yfeY at 4300.733 kb on + strand, within yfeY at 4300.733 kb on + strand, within yfeY at 4300.734 kb on - strand, within yfeY at 4300.734 kb on - strand, within yfeY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,298,530 + amiA OKFHMN_22100 0.32 +0.4 4,298,714 - amiA OKFHMN_22100 0.53 -0.3 4,298,837 - amiA OKFHMN_22100 0.67 +0.3 4,299,009 - amiA OKFHMN_22100 0.87 -0.3 4,299,138 + -0.1 4,299,159 + -2.9 4,299,160 - +0.4 4,299,160 - +0.1 4,299,162 - +0.0 4,299,173 + -1.9 4,299,210 - +0.0 4,299,225 - +0.8 4,299,334 + -0.1 4,299,351 + -0.6 4,299,445 + ypeA OKFHMN_22105 0.25 -0.2 4,299,446 - ypeA OKFHMN_22105 0.25 +0.4 4,299,548 + ypeA OKFHMN_22105 0.49 +2.0 4,299,549 - ypeA OKFHMN_22105 0.50 +1.4 4,299,549 - ypeA OKFHMN_22105 0.50 -2.6 4,299,549 - ypeA OKFHMN_22105 0.50 +0.2 4,299,805 + yfeZ OKFHMN_22110 0.12 -0.2 4,299,890 + yfeZ OKFHMN_22110 0.31 -1.3 4,299,912 + yfeZ OKFHMN_22110 0.36 +0.6 4,299,995 + yfeZ OKFHMN_22110 0.54 -2.6 4,300,023 + yfeZ OKFHMN_22110 0.61 -0.7 4,300,054 + yfeZ OKFHMN_22110 0.68 -1.3 4,300,055 - yfeZ OKFHMN_22110 0.68 -1.5 4,300,120 + yfeZ OKFHMN_22110 0.82 +0.5 4,300,276 + -0.5 4,300,276 + -0.7 4,300,289 + -0.1 4,300,332 - yfeY OKFHMN_22115 0.12 -1.5 4,300,406 - yfeY OKFHMN_22115 0.25 +0.5 4,300,691 + yfeY OKFHMN_22115 0.75 +1.0 4,300,730 + yfeY OKFHMN_22115 0.82 -1.2 4,300,731 - yfeY OKFHMN_22115 0.82 +0.8 4,300,733 + yfeY OKFHMN_22115 0.82 +0.1 4,300,733 + yfeY OKFHMN_22115 0.82 +0.3 4,300,733 + yfeY OKFHMN_22115 0.82 -0.3 4,300,734 - yfeY OKFHMN_22115 0.82 +0.0 4,300,734 - yfeY OKFHMN_22115 0.82 +1.8
Or see this region's nucleotide sequence