Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutS and eutP are separated by 12 nucleotides eutP and eutQ overlap by 26 nucleotides eutQ and eutT overlap by 4 nucleotides eutT and pta overlap by 4 nucleotides
OKFHMN_22010: eutS - Bacterial microcompartment shell protein EutS, at 4,282,650 to 4,282,985
eutS
OKFHMN_22015: eutP - ethanolamine utilization acetate kinase EutP, at 4,282,998 to 4,283,477
eutP
OKFHMN_22020: eutQ - ethanolamine utilization acetate kinase EutQ, at 4,283,452 to 4,284,153
eutQ
OKFHMN_22025: eutT - ethanolamine utilization cob(I)yrinic acid a,c-diamide adenosyltransferase EutT, at 4,284,150 to 4,284,881
eutT
OKFHMN_22030: pta - phosphate acetyltransferase, at 4,284,878 to 4,285,894
pta
Position (kb) 4283
4284
4285 Strain fitness (log2 ratio) -1
0
1
2 at 4282.593 kb on - strand at 4282.656 kb on - strand at 4282.778 kb on + strand, within eutS at 4282.778 kb on + strand, within eutS at 4282.778 kb on + strand, within eutS at 4282.786 kb on + strand, within eutS at 4283.171 kb on - strand, within eutP at 4283.171 kb on - strand, within eutP at 4283.377 kb on + strand, within eutP at 4283.418 kb on + strand, within eutP at 4283.538 kb on + strand, within eutQ at 4283.538 kb on + strand, within eutQ at 4283.539 kb on - strand, within eutQ at 4283.580 kb on + strand, within eutQ at 4283.833 kb on - strand, within eutQ at 4284.290 kb on + strand, within eutT at 4284.425 kb on + strand, within eutT at 4284.801 kb on + strand, within eutT at 4284.802 kb on - strand, within eutT at 4284.824 kb on - strand at 4284.945 kb on + strand at 4285.072 kb on - strand, within pta
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,282,593 - +0.3 4,282,656 - +0.3 4,282,778 + eutS OKFHMN_22010 0.38 +0.1 4,282,778 + eutS OKFHMN_22010 0.38 -0.2 4,282,778 + eutS OKFHMN_22010 0.38 +0.9 4,282,786 + eutS OKFHMN_22010 0.40 +1.2 4,283,171 - eutP OKFHMN_22015 0.36 +1.5 4,283,171 - eutP OKFHMN_22015 0.36 +2.1 4,283,377 + eutP OKFHMN_22015 0.79 +0.6 4,283,418 + eutP OKFHMN_22015 0.88 -0.3 4,283,538 + eutQ OKFHMN_22020 0.12 +0.0 4,283,538 + eutQ OKFHMN_22020 0.12 -0.2 4,283,539 - eutQ OKFHMN_22020 0.12 -0.2 4,283,580 + eutQ OKFHMN_22020 0.18 -1.5 4,283,833 - eutQ OKFHMN_22020 0.54 +2.2 4,284,290 + eutT OKFHMN_22025 0.19 -0.1 4,284,425 + eutT OKFHMN_22025 0.38 +0.6 4,284,801 + eutT OKFHMN_22025 0.89 -0.1 4,284,802 - eutT OKFHMN_22025 0.89 +0.9 4,284,824 - +1.6 4,284,945 + +0.3 4,285,072 - pta OKFHMN_22030 0.19 -0.8
Or see this region's nucleotide sequence