Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20775

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgshA and luxS are separated by 149 nucleotidesluxS and emrB are separated by 63 nucleotidesemrB and emrA are separated by 16 nucleotides OKFHMN_20760: gshA - glutamate--cysteine ligase, at 4,036,002 to 4,037,558 gshA OKFHMN_20770: luxS - S-ribosylhomocysteine lyase, at 4,037,708 to 4,038,223 luxS OKFHMN_20775: emrB - multidrug efflux MFS transporter permease subunit EmrB, at 4,038,287 to 4,039,825 emrB OKFHMN_20780: emrA - multidrug efflux MFS transporter periplasmic adaptor subunit EmrA, at 4,039,842 to 4,041,014 emrA Position (kb) 4038 4039 4040Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4037.357 kb on - strand, within gshAat 4037.363 kb on + strand, within gshAat 4037.417 kb on + strandat 4037.467 kb on + strandat 4037.718 kb on + strandat 4037.719 kb on - strandat 4037.719 kb on - strandat 4037.719 kb on - strandat 4037.896 kb on + strand, within luxSat 4037.896 kb on + strand, within luxSat 4037.912 kb on - strand, within luxSat 4037.969 kb on + strand, within luxSat 4037.971 kb on + strand, within luxSat 4038.099 kb on - strand, within luxSat 4038.233 kb on + strandat 4038.246 kb on - strandat 4038.278 kb on + strandat 4038.279 kb on - strandat 4038.279 kb on - strandat 4038.285 kb on - strandat 4038.285 kb on - strandat 4038.486 kb on + strand, within emrBat 4038.487 kb on - strand, within emrBat 4038.767 kb on - strand, within emrBat 4038.768 kb on + strand, within emrBat 4038.769 kb on - strand, within emrBat 4038.795 kb on + strand, within emrBat 4038.796 kb on - strand, within emrBat 4038.796 kb on - strand, within emrBat 4038.799 kb on + strand, within emrBat 4038.800 kb on - strand, within emrBat 4038.800 kb on - strand, within emrBat 4038.800 kb on - strand, within emrBat 4038.881 kb on - strand, within emrBat 4038.976 kb on - strand, within emrBat 4038.983 kb on - strand, within emrBat 4038.984 kb on + strand, within emrBat 4038.985 kb on - strand, within emrBat 4038.985 kb on - strand, within emrBat 4038.998 kb on - strand, within emrBat 4039.094 kb on - strand, within emrBat 4039.317 kb on - strand, within emrBat 4039.336 kb on + strand, within emrBat 4039.439 kb on - strand, within emrBat 4039.516 kb on + strand, within emrBat 4039.516 kb on + strand, within emrBat 4039.517 kb on - strand, within emrBat 4039.555 kb on + strand, within emrBat 4039.861 kb on - strandat 4039.861 kb on - strandat 4039.943 kb on + strandat 4039.943 kb on + strandat 4039.977 kb on - strand, within emrAat 4039.999 kb on - strand, within emrAat 4040.085 kb on - strand, within emrAat 4040.161 kb on + strand, within emrAat 4040.162 kb on - strand, within emrAat 4040.220 kb on - strand, within emrAat 4040.220 kb on - strand, within emrAat 4040.261 kb on + strand, within emrAat 4040.261 kb on + strand, within emrAat 4040.341 kb on + strand, within emrAat 4040.369 kb on + strand, within emrAat 4040.441 kb on + strand, within emrAat 4040.473 kb on + strand, within emrAat 4040.823 kb on - strand, within emrA

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,037,357 - gshA OKFHMN_20760 0.87 -1.6
4,037,363 + gshA OKFHMN_20760 0.87 -0.6
4,037,417 + -0.9
4,037,467 + -2.0
4,037,718 + -0.2
4,037,719 - +0.4
4,037,719 - -0.7
4,037,719 - +0.8
4,037,896 + luxS OKFHMN_20770 0.36 -0.0
4,037,896 + luxS OKFHMN_20770 0.36 +0.9
4,037,912 - luxS OKFHMN_20770 0.40 +0.8
4,037,969 + luxS OKFHMN_20770 0.51 +0.6
4,037,971 + luxS OKFHMN_20770 0.51 -2.0
4,038,099 - luxS OKFHMN_20770 0.76 -0.2
4,038,233 + -0.1
4,038,246 - -3.2
4,038,278 + -0.2
4,038,279 - -0.6
4,038,279 - +2.0
4,038,285 - +0.2
4,038,285 - +1.8
4,038,486 + emrB OKFHMN_20775 0.13 +0.0
4,038,487 - emrB OKFHMN_20775 0.13 +0.1
4,038,767 - emrB OKFHMN_20775 0.31 -0.1
4,038,768 + emrB OKFHMN_20775 0.31 -1.1
4,038,769 - emrB OKFHMN_20775 0.31 +0.8
4,038,795 + emrB OKFHMN_20775 0.33 -1.3
4,038,796 - emrB OKFHMN_20775 0.33 -0.5
4,038,796 - emrB OKFHMN_20775 0.33 +1.8
4,038,799 + emrB OKFHMN_20775 0.33 -0.6
4,038,800 - emrB OKFHMN_20775 0.33 -0.3
4,038,800 - emrB OKFHMN_20775 0.33 +0.0
4,038,800 - emrB OKFHMN_20775 0.33 +0.1
4,038,881 - emrB OKFHMN_20775 0.39 -2.3
4,038,976 - emrB OKFHMN_20775 0.45 +0.0
4,038,983 - emrB OKFHMN_20775 0.45 +0.7
4,038,984 + emrB OKFHMN_20775 0.45 -1.6
4,038,985 - emrB OKFHMN_20775 0.45 +0.8
4,038,985 - emrB OKFHMN_20775 0.45 +0.1
4,038,998 - emrB OKFHMN_20775 0.46 -0.7
4,039,094 - emrB OKFHMN_20775 0.52 -1.3
4,039,317 - emrB OKFHMN_20775 0.67 -0.4
4,039,336 + emrB OKFHMN_20775 0.68 +0.5
4,039,439 - emrB OKFHMN_20775 0.75 -0.7
4,039,516 + emrB OKFHMN_20775 0.80 -2.4
4,039,516 + emrB OKFHMN_20775 0.80 +0.0
4,039,517 - emrB OKFHMN_20775 0.80 -4.5
4,039,555 + emrB OKFHMN_20775 0.82 +0.3
4,039,861 - -1.0
4,039,861 - +0.6
4,039,943 + +0.3
4,039,943 + -1.1
4,039,977 - emrA OKFHMN_20780 0.12 +0.2
4,039,999 - emrA OKFHMN_20780 0.13 -3.3
4,040,085 - emrA OKFHMN_20780 0.21 -0.2
4,040,161 + emrA OKFHMN_20780 0.27 -0.0
4,040,162 - emrA OKFHMN_20780 0.27 +0.0
4,040,220 - emrA OKFHMN_20780 0.32 -3.5
4,040,220 - emrA OKFHMN_20780 0.32 +1.3
4,040,261 + emrA OKFHMN_20780 0.36 +0.5
4,040,261 + emrA OKFHMN_20780 0.36 +0.0
4,040,341 + emrA OKFHMN_20780 0.43 +0.1
4,040,369 + emrA OKFHMN_20780 0.45 -2.2
4,040,441 + emrA OKFHMN_20780 0.51 +0.3
4,040,473 + emrA OKFHMN_20780 0.54 +0.9
4,040,823 - emrA OKFHMN_20780 0.84 -1.8

Or see this region's nucleotide sequence