Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20635

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntascG and hydN are separated by 148 nucleotideshydN and hypF are separated by 152 nucleotideshypF and norW are separated by 127 nucleotides OKFHMN_20625: ascG - DNA-binding transcriptional regulator AscG, at 4,013,404 to 4,014,417 ascG OKFHMN_20630: hydN - electron transport protein HydN, at 4,014,566 to 4,015,093 hydN OKFHMN_20635: hypF - carbamoyltransferase HypF, at 4,015,246 to 4,017,498 hypF OKFHMN_20640: norW - NADH:flavorubredoxin reductase NorW, at 4,017,626 to 4,018,759 norW Position (kb) 4015 4016 4017 4018Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4014.254 kb on - strand, within ascGat 4014.888 kb on + strand, within hydNat 4014.953 kb on - strand, within hydNat 4014.994 kb on + strand, within hydNat 4015.148 kb on + strandat 4015.148 kb on + strandat 4015.366 kb on - strandat 4015.366 kb on - strandat 4015.382 kb on + strandat 4015.382 kb on + strandat 4015.383 kb on - strandat 4015.482 kb on + strand, within hypFat 4015.630 kb on - strand, within hypFat 4015.639 kb on - strand, within hypFat 4015.639 kb on - strand, within hypFat 4015.853 kb on + strand, within hypFat 4015.875 kb on - strand, within hypFat 4015.938 kb on + strand, within hypFat 4016.049 kb on + strand, within hypFat 4016.082 kb on + strand, within hypFat 4016.089 kb on + strand, within hypFat 4016.186 kb on + strand, within hypFat 4016.186 kb on + strand, within hypFat 4016.186 kb on + strand, within hypFat 4016.187 kb on - strand, within hypFat 4016.187 kb on - strand, within hypFat 4016.187 kb on - strand, within hypFat 4016.292 kb on - strand, within hypFat 4016.292 kb on - strand, within hypFat 4016.294 kb on + strand, within hypFat 4016.374 kb on + strand, within hypFat 4016.426 kb on + strand, within hypFat 4016.570 kb on - strand, within hypFat 4016.587 kb on + strand, within hypFat 4016.588 kb on - strand, within hypFat 4016.588 kb on - strand, within hypFat 4016.614 kb on + strand, within hypFat 4016.614 kb on + strand, within hypFat 4016.976 kb on - strand, within hypFat 4017.003 kb on + strand, within hypFat 4017.081 kb on - strand, within hypFat 4017.086 kb on - strand, within hypFat 4017.086 kb on - strand, within hypFat 4017.609 kb on + strandat 4017.627 kb on + strandat 4017.628 kb on - strandat 4017.762 kb on - strand, within norWat 4017.762 kb on - strand, within norWat 4017.800 kb on + strand, within norWat 4017.917 kb on + strand, within norWat 4017.954 kb on + strand, within norWat 4017.981 kb on + strand, within norWat 4017.986 kb on - strand, within norWat 4018.368 kb on + strand, within norWat 4018.385 kb on + strand, within norWat 4018.386 kb on - strand, within norWat 4018.386 kb on - strand, within norWat 4018.474 kb on + strand, within norWat 4018.475 kb on - strand, within norW

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,014,254 - ascG OKFHMN_20625 0.84 -1.6
4,014,888 + hydN OKFHMN_20630 0.61 +0.7
4,014,953 - hydN OKFHMN_20630 0.73 +0.5
4,014,994 + hydN OKFHMN_20630 0.81 +1.4
4,015,148 + -1.1
4,015,148 + -1.3
4,015,366 - +1.5
4,015,366 - -3.8
4,015,382 + -1.1
4,015,382 + -1.2
4,015,383 - -2.4
4,015,482 + hypF OKFHMN_20635 0.10 +0.4
4,015,630 - hypF OKFHMN_20635 0.17 -1.1
4,015,639 - hypF OKFHMN_20635 0.17 +1.0
4,015,639 - hypF OKFHMN_20635 0.17 -2.4
4,015,853 + hypF OKFHMN_20635 0.27 -0.3
4,015,875 - hypF OKFHMN_20635 0.28 +0.9
4,015,938 + hypF OKFHMN_20635 0.31 +0.6
4,016,049 + hypF OKFHMN_20635 0.36 -2.3
4,016,082 + hypF OKFHMN_20635 0.37 -3.0
4,016,089 + hypF OKFHMN_20635 0.37 -1.4
4,016,186 + hypF OKFHMN_20635 0.42 -0.1
4,016,186 + hypF OKFHMN_20635 0.42 -1.4
4,016,186 + hypF OKFHMN_20635 0.42 +0.2
4,016,187 - hypF OKFHMN_20635 0.42 +0.8
4,016,187 - hypF OKFHMN_20635 0.42 -3.7
4,016,187 - hypF OKFHMN_20635 0.42 +0.6
4,016,292 - hypF OKFHMN_20635 0.46 +0.7
4,016,292 - hypF OKFHMN_20635 0.46 +0.1
4,016,294 + hypF OKFHMN_20635 0.47 -1.2
4,016,374 + hypF OKFHMN_20635 0.50 +0.5
4,016,426 + hypF OKFHMN_20635 0.52 +0.5
4,016,570 - hypF OKFHMN_20635 0.59 +0.5
4,016,587 + hypF OKFHMN_20635 0.60 +0.4
4,016,588 - hypF OKFHMN_20635 0.60 -1.5
4,016,588 - hypF OKFHMN_20635 0.60 +0.6
4,016,614 + hypF OKFHMN_20635 0.61 -1.1
4,016,614 + hypF OKFHMN_20635 0.61 +0.0
4,016,976 - hypF OKFHMN_20635 0.77 +0.4
4,017,003 + hypF OKFHMN_20635 0.78 +0.3
4,017,081 - hypF OKFHMN_20635 0.81 -0.7
4,017,086 - hypF OKFHMN_20635 0.82 -2.3
4,017,086 - hypF OKFHMN_20635 0.82 -0.9
4,017,609 + -2.6
4,017,627 + -0.2
4,017,628 - +0.3
4,017,762 - norW OKFHMN_20640 0.12 -0.2
4,017,762 - norW OKFHMN_20640 0.12 +0.9
4,017,800 + norW OKFHMN_20640 0.15 +0.3
4,017,917 + norW OKFHMN_20640 0.26 +2.1
4,017,954 + norW OKFHMN_20640 0.29 +0.8
4,017,981 + norW OKFHMN_20640 0.31 -0.3
4,017,986 - norW OKFHMN_20640 0.32 -0.4
4,018,368 + norW OKFHMN_20640 0.65 -0.6
4,018,385 + norW OKFHMN_20640 0.67 -0.9
4,018,386 - norW OKFHMN_20640 0.67 +0.7
4,018,386 - norW OKFHMN_20640 0.67 -0.0
4,018,474 + norW OKFHMN_20640 0.75 -0.7
4,018,475 - norW OKFHMN_20640 0.75 -0.0

Or see this region's nucleotide sequence