Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20600

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycE and hycF are separated by 9 nucleotideshycF and hycG overlap by 1 nucleotideshycG and hycH overlap by 4 nucleotideshycH and hycI overlap by 8 nucleotideshycI and ascB are separated by 123 nucleotides OKFHMN_20590: hycE - formate hydrogenlyase subunit HycE, at 4,006,235 to 4,007,944 hycE OKFHMN_20595: hycF - formate hydrogenlyase subunit HycF, at 4,007,954 to 4,008,496 hycF OKFHMN_20600: hycG - formate hydrogenlyase subunit HycG, at 4,008,496 to 4,009,263 hycG OKFHMN_20605: hycH - formate hydrogenlyase assembly protein HycH, at 4,009,260 to 4,009,670 hycH OKFHMN_20610: hycI - hydrogenase maturation peptidase HycI, at 4,009,663 to 4,010,133 hycI OKFHMN_20615: ascB - 6-phospho-beta-glucosidase AscB, at 4,010,257 to 4,011,681 ascB Position (kb) 4008 4009 4010Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4007.515 kb on + strand, within hycEat 4007.536 kb on + strand, within hycEat 4007.537 kb on - strand, within hycEat 4007.549 kb on + strand, within hycEat 4007.550 kb on - strand, within hycEat 4007.620 kb on + strand, within hycEat 4007.625 kb on - strand, within hycEat 4007.715 kb on - strand, within hycEat 4007.715 kb on - strand, within hycEat 4007.766 kb on - strand, within hycEat 4007.866 kb on + strandat 4007.942 kb on + strandat 4007.960 kb on - strandat 4008.364 kb on + strand, within hycFat 4008.478 kb on + strandat 4008.507 kb on - strandat 4008.741 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.917 kb on - strand, within hycGat 4008.917 kb on - strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.144 kb on - strand, within hycGat 4009.152 kb on - strand, within hycGat 4009.254 kb on + strandat 4009.347 kb on + strand, within hycHat 4009.348 kb on - strand, within hycHat 4009.350 kb on + strand, within hycHat 4009.494 kb on + strand, within hycHat 4009.494 kb on + strand, within hycHat 4009.592 kb on + strand, within hycHat 4009.603 kb on + strand, within hycHat 4009.604 kb on - strand, within hycHat 4009.607 kb on + strand, within hycHat 4009.620 kb on - strand, within hycHat 4009.641 kb on + strandat 4009.641 kb on + strandat 4009.641 kb on + strandat 4009.676 kb on + strandat 4009.759 kb on - strand, within hycIat 4009.933 kb on - strand, within hycIat 4009.933 kb on - strand, within hycIat 4009.974 kb on + strand, within hycIat 4009.975 kb on - strand, within hycIat 4010.146 kb on + strandat 4010.146 kb on + strandat 4010.147 kb on - strandat 4010.255 kb on - strandat 4010.259 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,007,515 + hycE OKFHMN_20590 0.75 +1.2
4,007,536 + hycE OKFHMN_20590 0.76 +0.1
4,007,537 - hycE OKFHMN_20590 0.76 -1.0
4,007,549 + hycE OKFHMN_20590 0.77 -0.2
4,007,550 - hycE OKFHMN_20590 0.77 +0.2
4,007,620 + hycE OKFHMN_20590 0.81 -0.1
4,007,625 - hycE OKFHMN_20590 0.81 +0.1
4,007,715 - hycE OKFHMN_20590 0.87 +1.0
4,007,715 - hycE OKFHMN_20590 0.87 +0.5
4,007,766 - hycE OKFHMN_20590 0.90 -3.2
4,007,866 + -0.1
4,007,942 + +0.3
4,007,960 - +0.5
4,008,364 + hycF OKFHMN_20595 0.76 -0.2
4,008,478 + -0.0
4,008,507 - -0.4
4,008,741 - hycG OKFHMN_20600 0.32 -1.5
4,008,830 - hycG OKFHMN_20600 0.43 +0.7
4,008,830 - hycG OKFHMN_20600 0.43 +0.3
4,008,916 + hycG OKFHMN_20600 0.55 -0.6
4,008,916 + hycG OKFHMN_20600 0.55 +1.2
4,008,917 - hycG OKFHMN_20600 0.55 -0.8
4,008,917 - hycG OKFHMN_20600 0.55 +0.5
4,009,138 + hycG OKFHMN_20600 0.84 -0.5
4,009,138 + hycG OKFHMN_20600 0.84 +0.2
4,009,138 + hycG OKFHMN_20600 0.84 +0.8
4,009,144 - hycG OKFHMN_20600 0.84 -0.2
4,009,152 - hycG OKFHMN_20600 0.85 +0.7
4,009,254 + +0.7
4,009,347 + hycH OKFHMN_20605 0.21 +0.2
4,009,348 - hycH OKFHMN_20605 0.21 +0.2
4,009,350 + hycH OKFHMN_20605 0.22 +0.5
4,009,494 + hycH OKFHMN_20605 0.57 -0.4
4,009,494 + hycH OKFHMN_20605 0.57 +0.2
4,009,592 + hycH OKFHMN_20605 0.81 +0.4
4,009,603 + hycH OKFHMN_20605 0.83 -0.3
4,009,604 - hycH OKFHMN_20605 0.84 -3.7
4,009,607 + hycH OKFHMN_20605 0.84 -0.2
4,009,620 - hycH OKFHMN_20605 0.88 -3.1
4,009,641 + -1.3
4,009,641 + -4.1
4,009,641 + -0.1
4,009,676 + -0.1
4,009,759 - hycI OKFHMN_20610 0.20 +0.1
4,009,933 - hycI OKFHMN_20610 0.57 -1.0
4,009,933 - hycI OKFHMN_20610 0.57 +0.2
4,009,974 + hycI OKFHMN_20610 0.66 -0.3
4,009,975 - hycI OKFHMN_20610 0.66 +0.1
4,010,146 + +0.5
4,010,146 + -1.1
4,010,147 - +1.2
4,010,255 - +0.1
4,010,259 - +0.2

Or see this region's nucleotide sequence