Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt edcC and ecdD are separated by 10 nucleotides ecdD and pphB are separated by 40 nucleotides pphB and mutS are separated by 105 nucleotides
OKFHMN_20510: edcC - Phenolic acid decarboxylase, at 3,990,575 to 3,992,002
edcC
OKFHMN_20515: ecdD - Protein EcdD, at 3,992,013 to 3,992,249
ecdD
OKFHMN_20520: pphB - protein-serine/threonine phosphatase, at 3,992,290 to 3,992,946
pphB
OKFHMN_20525: mutS - DNA mismatch repair protein MutS, at 3,993,052 to 3,995,613
mutS
Position (kb)
3992
3993 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3991.094 kb on - strand, within edcC at 3991.213 kb on + strand, within edcC at 3991.235 kb on + strand, within edcC at 3991.236 kb on - strand, within edcC at 3991.410 kb on - strand, within edcC at 3991.488 kb on - strand, within edcC at 3991.551 kb on - strand, within edcC at 3991.551 kb on - strand, within edcC at 3991.602 kb on - strand, within edcC at 3991.602 kb on - strand, within edcC at 3991.625 kb on - strand, within edcC at 3991.625 kb on - strand, within edcC at 3991.704 kb on - strand, within edcC at 3991.704 kb on - strand, within edcC at 3991.708 kb on - strand, within edcC at 3991.996 kb on + strand at 3991.997 kb on - strand at 3992.001 kb on - strand at 3992.087 kb on - strand, within ecdD at 3992.088 kb on + strand, within ecdD at 3992.109 kb on + strand, within ecdD at 3992.109 kb on + strand, within ecdD at 3992.109 kb on + strand, within ecdD at 3992.109 kb on + strand, within ecdD at 3992.112 kb on - strand, within ecdD at 3992.809 kb on - strand, within pphB at 3992.846 kb on - strand, within pphB at 3993.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,991,094 - edcC OKFHMN_20510 0.36 +0.6 3,991,213 + edcC OKFHMN_20510 0.45 -3.9 3,991,235 + edcC OKFHMN_20510 0.46 +0.2 3,991,236 - edcC OKFHMN_20510 0.46 -0.1 3,991,410 - edcC OKFHMN_20510 0.58 -0.2 3,991,488 - edcC OKFHMN_20510 0.64 -2.6 3,991,551 - edcC OKFHMN_20510 0.68 -0.4 3,991,551 - edcC OKFHMN_20510 0.68 +0.4 3,991,602 - edcC OKFHMN_20510 0.72 -0.4 3,991,602 - edcC OKFHMN_20510 0.72 -0.1 3,991,625 - edcC OKFHMN_20510 0.74 -0.8 3,991,625 - edcC OKFHMN_20510 0.74 +0.1 3,991,704 - edcC OKFHMN_20510 0.79 +1.3 3,991,704 - edcC OKFHMN_20510 0.79 +0.6 3,991,708 - edcC OKFHMN_20510 0.79 +0.9 3,991,996 + -1.4 3,991,997 - -0.6 3,992,001 - -1.0 3,992,087 - ecdD OKFHMN_20515 0.31 -0.0 3,992,088 + ecdD OKFHMN_20515 0.32 +0.6 3,992,109 + ecdD OKFHMN_20515 0.41 -0.2 3,992,109 + ecdD OKFHMN_20515 0.41 +0.8 3,992,109 + ecdD OKFHMN_20515 0.41 +1.4 3,992,109 + ecdD OKFHMN_20515 0.41 -0.0 3,992,112 - ecdD OKFHMN_20515 0.42 -1.1 3,992,809 - pphB OKFHMN_20520 0.79 +0.3 3,992,846 - pphB OKFHMN_20520 0.85 +0.4 3,993,008 + -0.0
Or see this region's nucleotide sequence